THE EFFECT OF BACTERIZATION AND PRE-SOWING SEED TREATMENT WITH BENORAD ON THE GROWTH OF SOYBEAN PLANTS AND THE REALIZATION OF THE SYMBIOTIC POTENTIAL OF PESTICIDE RESISTANT RHIZOBIA
DOI: http://dx.doi.org/10.30970/sbi.1701.705
Abstract
Background. Bacterial fertilizers based on active strains of nitrogen-fixing microorganisms and fungicides for pre-sowing seed dressing are increasingly used in soybean cultivation technologies. Given the usefulness of combining the specified processes, the selection of chemical and biological preparations for the combined processing of seed material is relevant.
Materials and Methods. In vegetation experiments, the effect of treatment of soybean seeds with benorad and inoculation with nodule bacteria resistant to fungicides in pure culture, obtained by the methods of analytical selection and transposon mutagenesis, on plant growth, the number and weight of root nodules and their nitrogenase activity was studied. Physiological, microbiological, statistical methods and gas chromatography were used.
Results. Under the complex application of inoculants and benorad, we recorded a decrease in the weight of the aerial part of soybeans by 8.7–20.9 % and the weight of roots by 4.8–16.8 % during the growing season compared to control plants (regardless of the strain of rhizobia used for bacterization). In the case of seeds dressing, the dynamics of the formation of the number of root nodules by the B. japonicum B78 differed from other inoculant strains that were used in the research. This indicator decreased by 20.6 and 16.3% at the stage of three true leaves and budding–beginning of flowering and increased (by 28.0 %) at the stage of pods formation compared to control plants. The negative effect of seed dressing on the nitrogenase activity of symbiotic systems was observed after the application of benorad, at the stage of three true leaves and budding–beginning of flowering was noted. The degree of inhibitory effect of the chemical preparation on intensity of N2 assimilation depended on the properties of microsymbionts. In the stage of pods formation, the level of N2 fixation by soybean root nodules formed by the B. japonicum PC07 and B144 during seed dressing exceeded the corresponding level in the control plants by 32.2 and 45.7 %, respectively.
Conclusions. The use of microbial preparations for inoculation of soybean seeds, made on the basis of nodule bacteria strains with high resistance to chemical plant protection agents, allows for a gradual reduction of the toxic effect of artificially synthesized compounds on the formation and functioning of symbiotic systems.
Keywords
Full Text:
PDFReferences
Borzykh, О. (2015). Khvoroby roslyn osnovnykh polovykh kultur v ahrotsenozakh Ukrainy [Plant diseases of the main fieldcrops in agrocenosis of Ukraine]. Biological Resources and Nature Management, 7(1-2), 183-189. (In Ukrainian) Google Scholar | ||||
| ||||
Bushulian, O., & Bushulian, M. (2013). Sortova reaktsiia nutu na obrobku suchasnymy protruinykamy [Varietal reaction of chickpeas to treatment with modern fungicides]. Bulletin of the Lviv National Agrarian University. Series: Agronomy, 17(2), 355-359. (In Ukrainian) Google Scholar | ||||
| ||||
Fostolovych, S. I. (2013). Kormova produktyvnist posiviv soi zalezhno vid kontroliu fitosanitarnoi sytuatsii v umovakh Lisostepu Pravoberezhnoho [Fodder productivity of soybean crops depending on the control of the phytosanitary situation in the conditions of the Right-Bank Forest-Steppe]. Feeds and Feed Production, 77, 148-152. (In Ukrainian) Crossref | ||||
| ||||
Gaur, A., Kumar, A., Kiran, R., & Kumari, P. (2020). Importance of seed-borne diseases of agricultural crops: economic losses and impact on society. In R. Kumar & A. Gupta (Eds.), Seed-borne diseases of agricultural crops: detection, diagnosis & management (pp. 3-23). Singapore: Springer. doi:10.1007/978-981-32-9046-4 Crossref ● Google Scholar | ||||
| ||||
Hardy, R. W. F., Holsten, R. D., Jackson, E. K., & Burns, R. C. (1968). The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiology, 42(8), 1185-1207. doi:10.1104/pp.43.8.1185 Crossref ● PubMed ● PMC ● Google Scholar | ||||
| ||||
Igiehon, N. O., & Babalola, O. O. (2018). Rhizosphere microbiome modulators: contributions of nitrogen fixing bacteria towards sustainable agriculture. International Journal of Environmental Research and Public Health, 15(4), 574. doi:10.3390/ijerph15040574 Crossref ● PubMed ● PMC ● Google Scholar | ||||
| ||||
Kots, S. Ya., Morgun, V. V., Tikhonovich, I. A., Provorov, N. A., Patyka, V. F., Petrychenko, V. F., Melnykova, N. N., & Mamenko, P. N. (2011). Biologicheskaya fiksatsiya azota: genetika azotfiksatsii, geneticheskaya inzheneriya shtammov [Biological nitrogen fixation: genetics of nitrogen fixation, genetic engineering of strains]. Vol. 3. Kyiv: Logos. (In Russian) Google Scholar | ||||
| ||||
Kukol, K. P., Vorobey, N. A., & Kots, S. Y. (2019). Chutlyvist chystykh kultur Bradyrhizobium japonicum do funhitsydiv [Sensitivity of pure cultures of Bradyrhizobium japonicum to fungicides]. Agriciltural Microbiology, 30, 20-31. (In Ukrainian) doi:10.35868/1997-3004.30.20-31 Crossref ● Google Scholar | ||||
| ||||
Lamichhane, J. R., You, M. P., Laudinot, V., Barbetti, M. J., & Aubertot, J.-N. (2020). Revisiting sustainability of fungicide seed treatments for field crops. Plant Disease, 104(3), 610-623. doi:10.1094/pdis-06-19-1157-fe Crossref ● PubMed ● Google Scholar | ||||
| ||||
Nasreen, N. (2003). Effect of fungicides in limiting the growth of seed borne fungi of soybean. Pakistan Journal of Plant Pathology, 2(2), 119-122. doi:10.3923/ppj.2003.119.122 Crossref ● Google Scholar | ||||
| ||||
Ohyama, T. (2017). The role of legume-Rhizobium symbiosis in sustainable agriculture. In S. Sulieman & L. S. Tran (Eds.), Legume nitrogen fixation in soils with low phosphorus availability (pp. 1-20), Berlin: Springer, Cham. doi:10.1007/978-3-319-55729-8_1 Crossref ● Google Scholar | ||||
| ||||
Pavlyshche, A. V., Kiriziy, D. A., & Kots, S. Ya. (2017). Reaktsiia symbiotychnykh system soi na diiu funhitsydiv za riznykh sposobiv obrobky [The reaction of symbiotic soybean systems to the action of fungicides under various treatment]. Plant Physiology and Genetics, 49(3), 237-247. doi:10.15407/frg2017.03.237 (In Ukrainian) Crossref ● Google Scholar | ||||
| ||||
Petrychenko, V. F., Lykhochvor, V. V., Ivaniuk, S. V., Korniichuk, O. V., Kolisnyk, S. I., Kobak, S. Ya., ... & Zakharova, O. M. (2016). Soia [Soybean]. Vinnytsia: Dilo. (In Ukrainian) Google Scholar | ||||
| ||||
Sagitov, A. O., Uspanov, A. M., Sarsenbaeva, G. B., Bekezhanova, M., Ussembayeva, Z. S., & Tussupbayev, K. B. (2020). Pre-sowing treatment of soybean seeds against seed infection. Ecology, Environment and Conservation, 26(2), 507-513. Google Scholar | ||||
| ||||
Shahid, M., & Khan, Mohd. S. (2019). Fungicide tolerant Bradyrhizobium japonicum mitigate toxicity and enhance greengram production under hexaconazole stress. Journal of Environmental Sciences, 78, 92-108. doi:10.1016/j.jes.2018.07.007 Crossref ● PubMed ● Google Scholar | ||||
| ||||
Shahid, M., Khan, M. S., & Kumar, M. (2019). Kitazin-pea interaction: understanding the fungicide induced nodule alteration, cytotoxicity, oxidative damage and toxicity alleviation by Rhizobium leguminosarum. RSC Advances, 9(30), 16929-16947. doi:10.1039/c9ra01253b Crossref ● PubMed ● PMC ● Google Scholar | ||||
| ||||
Shea, Z., Singer, W. M., & Zhang, B. (2020). Soybean production, versatility, and improvement. In M. Hasanuzzaman (Ed.), Legume crops-prospects, production and uses (pp. 29-47). IntechOpen. doi:10.5772/intechopen.90304 Crossref ● Google Scholar | ||||
| ||||
State register of plant varieties suitable for dissemination in Ukraine. (2023, February). Retrieved from https://minagro.gov.ua/file-storage/reyestr-sortiv-roslin (In Ukrainian) | ||||
| ||||
Vorobey, N. А., Kukol, K. P., & Kots, S. Ya. (2020). Otsinka toksychnosti vplyvu funhitsydiv na bulbochkovi bakterii Bradyrhizobium japonicum u chystii kulturi [Fungicides toxicity assessment on Bradyrhizobium japonicum nodule bacteria in pure culture]. Mikrobiolohichnyi Zhurnal, 82(3), 45-54. doi:10.15407/microbiolj82.03.045 (In Ukrainian) Crossref ● Google Scholar | ||||
| ||||
Whitham, S. A., Qi, M., Innes, R. W., Ma, W., Lopes-Caitar, V., & Hewezi, T. (2016). Molecular soybean-pathogen interactions. Annual Review of Phytopathology, 54(1), 443-468. doi:10.1146/annurev-phyto-080615-100156 Crossref ● PubMed ● Google Scholar | ||||
| ||||
Yashchuk, V. U., Ivanov, D. V., Kryvosheia, R. M., Tsybulniak, Yu. O., & Koretskyi, A. P. (2022). Perelik pestytsydiv i ahrokhimikativ, dozvolenykh do vykorystannia v Ukraini [List of pesticides and agrochemicals, allowed for use in Ukraine]. Kyiv: Yunivest Media (In Ukrainian) |
Refbacks
- There are currently no refbacks.
Copyright (c) 2023 P. P. Pukhtaievych, K. P. Kukol, N. A. Vorobey, S. Ya. Kots
This work is licensed under a Creative Commons Attribution 4.0 International License.