N. P. Harasym, O. I. Bishko, S. M. Mandzynets, A. B. Heneha, M. B. Halan, V. P. Otchych, D. I. Sanahursky



The effects of histamine in 1 mg/kg and 8 mg/kg doses that correspond to the doses causing pathological effects at experimental conditions and of sodium hypochlorite in 5 mg/l dose – the lowest concentration of sodium hypochlorite that affects a body by oral administration, on the key enzymes of blood plasma antioxidant system were studied. It was found that histamine used in both concentrations intensified superoxide dismutase activity for 14 days. The simultaneous injections of histamine and sodium hypochlorite caused significant activation of superoxide dismutase in rats. Sodium hypochlorite received by rats with drinking solution caused the same effect. The catalase activity of blood plasma was not significantly affected by histamine, and its activity was significantly increased only under the influence of biogenic amine in 1 mg/kg dose on the 7th day of the experiment. Sodium hypochlorite caused a decline in catalase activity both in intact animals and in animals that received histamine injections subcutaneously. The injection of histamine in 1 mg/kg dose caused an increase in glutathione peroxidase activity on the 1st and 7th day of the experiment. Histamine in 8 mg/kg dose caused the intensification of glutathione peroxidase activity only on the 1st day, followed by the inhibition on the 14th day of the experiment. Sodium hypochlorite received by rats with drin­king solution led to general lowering of glutathione peroxydase activity in blood plasma.


rats, histamine, sodium hypochlorite, blood plasma, superoxide dismutase, catalase, glutation peroxydase

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1. Bishko O.I., Harasym N.P., Sanahurskyj D.I. Antioxidant defense system state in blood plasma and heart muscle of rats under the influence of histamine and sodium hypoclorite. The Ukrainian Biochemical Journal, 2014; 86(6): 56-65. (In Ukrainian)

2. Bishko O.I., Harasym N.P., Sanahurskyj D.I. The system of antioxidant defence in liver and kidneys of rat at influence of histamine and sodium hypochlorite. Experimental physiology and biochemistry, 2014; 3: 33-43. (In Ukrainian)

3. Chekman I.S. Clinical pharmacology of antihistamin drugs. Medicine of Railway Transport of Ukraine, 2002; 2: 58-61. (In Ukrainian)

4. Golovchak N.P., Tarnovska A.V., Sanagurski D.I. Lipid peroxidation in living organisms L: Ivan Franko National University of Lviv, 2012. 250 p. (In Ukrainian)

5. Hidalgo E., Bartolome R., Dominguez C. Cytotoxicity mechanisms of sodium hypochlorite in cultured human dermal fibroblasts and its bactericidal effectiveness. Chemico-Biological Interactions, 2002; 139: 265-282.

6. Iskusnykh A.Y., Basharina O.V., Artyukhov V.G. et al. Effect of histamine on the functional properties of neutrophils and intensity of the process of lipid peroxidation in blood donors. Vestnik VSU Series: Chemistry. Biology. Pharmacy, 2008: 1: 93-96. (In Russian)

7. Khavrona O., Byletska L. The effect of proinflammatory enzymes inhibitors on functioning of l-arginine/no system and immunological parameters in blood of rats under the experimental colitis. Studia Biologica, 2016; 10(1): 45-52. (In Ukrainian).

8. Komarenko V.I., Terekhov A.A., Vorobyova A.P. et al. Investigation of the role of H1-receptor responses in rat liver portal vessels to histamine. B. Khmelnitsky Cherkasy Nat. Univ. Biol. Sci. Series, 2008; 128: 54-58. (In Ukrainian)

9. Koroljuk M.A., Ivanovа L.I., Mayorov I.G. Method for determination of catalase activity. Labor. Work, 1988; 1: 16-19. (In Russian)

10. Kostyuk V.A., Potapovich A.I., Kovalevа J.M. Simple and sensitive method for the determi­nation of SOD, based on the oxidation of quercetin. Probl. Med. Chem, 1990; 36(2): 88-91. (In Russian)

11. Lowry O.H. Protein measurement with the Folin phenol reagent. J. Biol. Chem, 1951; 193(1): 404-415.

12. Moin V.M. A simple and specific method for determining the activity of glutathione peroxidase in erythrocytes. Lab. Delo, 1986; 2: 724-727. (In Russian)

13. Naumova O.A. Syndrome, low tolerance to histamine. Kindergarten Doctor, 2013; 3(24): 44-50. (In Ukrainian)

14. Petrov S.I. The use of sodium hypochlorite in clinical toxicology. The thesis for the degree of doctor of medical sciences. Moscow, 2005. 197 p. (In Russian)

15. Shahid M., Tripathi T., Sobia F. et al. Histamine, Histamine Receptors, and their Role in Immunomodulation: An Updated Systematic Review. The Open Immunology Journal, 2009; 2: 9-41.

16. Wagner C., Eckman J., Wick T. Histamine increases sickle erythrocyte adherence to endothelium. British Jornal of Haematology, 2005; 132: 512-522.


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