THE INFLUENCE OF THE REGOPLANT AND STIMPO ON THE ACTIVITY OF ANTIOXIDANT PROTECTION ENZYMES IN THE HELIANTHUS ANNUUS L. AND BRASSICA NAPUS L. GROWTH ON THE SUBSTRATIONS OF THE WET RECOVERY OF COAL MINE

S. Yu. Makogonenko, V. I. Baranov, O. I. Terek


DOI: http://dx.doi.org/10.30970/sbi.1201.539

Abstract


The influence of optimal growth rates of G.A. and new growth regulators of Stimpo and Régoplant on the activity of antioxidant protection enzymes – peroxidase, catalase and polyphenol oxidase, as well as the content of hydrogen peroxide and phenols in the seedlings of Helianthus annuus L. and Brassica napus L. due to their growth on extracts from the substrates of the waste heap of coal mines. At influence the studied regulators on plants revealed increased activity of peroxidase, decreased activity of catalase and polyphenoxidase and increased content of hydrogen peroxide. The content of phenolic compounds decreased due to increased activity of peroxidase. The obtained results indicate the changes in the activity of antioxidant enzymes at optimal growth growth concentrations and their promising application for stimulating plant growth during phytorecultivation treatment of the waste heaps.


Keywords


Stimpo, Régoplant, gibberellic acid, substrates of coal mines, Helianthus annuus L., Brassica napus L.

References


1. Babayants A.V., Gritsenko Z.M., Ponomarenko S.P. Biostimulators (growth regulators) of plants. Kyiv: ISTC Agrobiotech, 2013-2014. P. 3-15. (In Ukrainian)

2. Bakun V.R., Kovalska O.R., Patsula O.I. The protective role of treptolem in rape and sunflower plants for the action of lead ions. Digest of theses IV International sciences conf. students and graduate students "Youth and the progress of biology". Lviv, 2008. P. 373-374. (In Ukrainian)

3. Baranov V.I. Ecological description of the waste heap of coal mines of the CEP JSC "Lviv­systemenergo" as an object for planting greenery. Visnyk of Lviv University. Biological Series, 2008; 46: 172-178.

4. Baranov V.I., Gavrilyak M.Ya., Telegus Ya.V. Changes in the content of sulfur, free amino acids and protein in rapeseed plants, fertilized with encapsulated fertilizers on substrates of a coal dumps waste mine. Studia Biologica, 2010; 4(1): 53-62. (In Ukrainian)
https://doi.org/10.30970/sbi.0401.069

5. Baranov V., Beshley S., Telegus Y. Some biochemical indices of the adaptation of the calcareous rootstock (Calamagrostis epigeios (L.) Roth) to the conditions of the edaphops of coal mines dams. Visnyk of Lviv University. Biological Series, 2012; 58: 292-299. (In Ukrainian)

6. Baranov V.I., Knysh I.M., Blida I.A. et al. Orchid ordinary - phytoreemedient of heavy metals in drainage ditches of waste heaps of coal mines. Studia Biologica, 2012; 6(1): 93-100. (In Ukrainian)
https://doi.org/10.30970/sbi.0601.188

7. Bilchuk V.S., Vinnichenko O.M., Shupranova L.V. The activity and isoenzymatic composition of corn seedlings polyphenoloxidase under the influence of lead ions and cadmium. Visnyk of Dnipropetrovsk University, 2002; 10(2): 120-126. (In Ukrainian)

8. Boyarkin A.N. A quick method for determining the activity of peroxidase and polyphenol oxidase (modified) Proceedings of the Institute of Plant Physiology of the USSR Academy of Sciences, 1954; 8(2): 398-403. (In Russian).

9. Chechuy O.F. The content of phenolic compounds in the Glicine max L. seed in germination under the conditions of oxidative stress caused by the influence of ions of cobalt and cadmium. Visnyk of Uzhgorod University. Biological Series, 2011; 30: 197-200. (In Ukrainian)

10. Dovganich N.V. Activity of catalase and peroxidase of leaves of celandine in the zone of influence of the Burshtyn thermal power plant. Archive of Clinical Medicine, 2014; 1: 23-26. (In Ukrainian)

11. Garifzianov A.P., Zhukov N.N., Ivanishchev V.V. Physical Formation and Oxygen Reactions of Active Oxygen Formations in Plant Cells. Modern Problems of Science and Education, 2011; 2. 0421100037M3037. (In Russian)

12. Gashchyshyn V.R., Patsula O.I., Terek O.I. Influence of zinc and copper ions on the content of hydrogen peroxide and activity of catalase and peroxidase of plants Brassica napus L. Ukraine Botanical Journal, 2012; 69(5): 743-750. (In Ukrainian)

13. Gashchyshyn V.R., Patsula O.I., Terek O.I. Accumulation of heavy metals in Brassica napus L. and Helianthus annuus L. under the influence of zinc salts and growth regulator of treptolem. Physiology of Plants and Genetics. Lviv, 2014; 46 (4): 343-350. (In Ukrainian)

14. Genik Ya.V. Disturbed territories of Lviv region and ways of their phytomelioration and recultivation. Visnyk of Kyiv National Taras Shevchenko University, 2008; 18(10): 22-26. (In Ukrainian)

15. GOST 12038-84. Methods for determining the germination and germination energy. Seeds of agricultural crops. Publishing Standards. Moscow, 1984; 1: 57. (In Ukrainian)

16. Gritsenko Z.M., Ponomarenko S.P. et al. Biologically active substances in crop production. Uman, 2008; 179-191. (In Ukrainian)

17. Jaspers P., Kangasjarvi J. Reactive oxygen species in abiotic stress signalling. Physiol. Plant, 2010; 138: 405-413.
https://doi.org/10.1111/j.1399-3054.2009.01321.x
PMid:20028478

18. Juk I.V., Dmitriev O.P., Lisova G.M. The role of peroxidase in the formation of induced resistance of wheat plants using immunomodulators. Factors of Experimental Evolution of Organisms, 2016; 18: 81-84. (In Ukrainian)

19. Knish I.B. Kharkevich V.V. The distribution of the content of chemical elements in the rocks of the heaps of the Chervonohrad mining and industrial district. Visnyk of Lviv University. Geological Series, 2003; 17: 148-158. (In Ukrainian)

20. Kolupaev Yu.E., Karpets Yu.V. Formation of adaptive plant responses to the action of abiotic stressors. Basis. Kyiv, 2010; 351. (In Ukrainian)

21. Kolupaev Yu.E., Karpets Yu.V., Obozny A.I. Antioxidant system of plants: participation in cellular signaling and adaptation to the action of stressors. Visnyk of Kharkiv National Agrarian University. Biological Series, 2011; 1: 6-34. (In Russian)

22. Kolupaev Yu.E., Oboznii O.I. Active forms of oxygen and antioxidant system at cross-adaptation of plants to the action of abiotic stressors. Visnyk of Kharkiv National Agrarian University. Biological Series, 2013; 3: 18-31. (In Ukrainian)

23. Korolyuk M.A., Ivanova L.I., Mayorova I.G., Tokarev V.E. Method for determining the activity of catalase. Laboratory Business, 1988; 1: 6-19. (In Russian)

24. Kreslavsky V.D., Los' D.A., Allahverdiev S.I., Kuznetsov Vl.V. The Signal Role of Active Oxygen Forms in Stress in Plants. Physiology of Plants, 2012; 59(2): 63-178. (In Russian)
https://doi.org/10.1134/S1021443712020057

25. Kurganova L.N., Veselov A.P., Sinitsina Yu.V., Elikova E.A. Products of peroxidation of lipids as potential intermediaries between the effects of elevated temperature and the development of stress - reaction of plants. Plant Physiology, 1999; 46(2): 218-222. (In Russian)

26. Kuznetsova Vl.V., Kuznetsova V.V., Romanov G.A. Molecular-genetic and biochemical me­thods of modern plant biology. Methods in Biology. Laboratory of Knowledge. Moscow, 2012. 487 p. (In Russian)

27. Lukina N.V., Chibrik T.S., Glasyrina M.A., Filimonova E.I. Biological recultivation and monitoring of disturbed land industry. Biological Systems. Yekaterinbourg, 2008; 6(2): 256. (In Russian)

28. Merzlyak M.N. Activated oxygen and oxidative processes in plant cell membranes. Itogi Nauki i Tekhniki. Plant Physiology Series, 1989; 6: 167. (In Russian)

29. Oliynyk O., Furman V.M., Solodka T.M., Vakulenchuk S.I. Study of the effectiveness of pre-seed treatment of seeds by growth stimulators of plants. Journal of the National University of Water Management and Nature Management, 2013; 4(64): 112-118. (In Ukrainian)

30. Rossikhina-Galich G.S., Likholat Yu.V., Sergey O.I., Grigoryuk I.P. Activity of enzymes of catalase and peroxidase in leaves and roots of seedlings of maize genotypes under the influence of soil herbicides and drought. Scientific Reports of the National University of Bioresources and Nature Management of Ukraine, 2015; 6. (In Ukrainian)

31. Sichkar S.V., Korobkova K.S. Activity of oxidative enzymes of plant cells in conditions of experimental mycoplasmosis. Microbial Journal, 2011; 73(2): 38-43. (In Ukrainian)

32. Smetanin V.I. Land reclamation: a review of technologies. Ecology and Industry of Russia, Moscow, 2004; 42-45. (In Russian)

33. Tarchevsky I.A. Signal systems of plant cells. Science, Moscow, 2002; 294. (Russian)

34. Trachtenberg I. Experience in studying the biogeochemical province of one of the mining regions of Western Ukraine: A book on poisons and poisonings. Sketches of toxicology. Kiev: Naukova Dumka, 2000. P. 213-225. (In Russian)

35. Voytsekhivska O.V., Sitar O.V., Taran N.Yu. Phenolic compounds: diversity, biological activity, prospects of application, Visnyk of Kharkiv University. Biological Series, 2015; 1(34): 104-119. (In Ukrainian)

36. Vranova E., Inze D., Breusegem F. Signal transduction during oxidative stress, J. Exp. Bot, 2002; 53: 1227-1236.
https://doi.org/10.1093/jexbot/53.372.1227
PMid:11997371

37. Zaprometov M.N. Biochemical methods in plant physiology. Moscow: Science, 1971. 191 p. (In Russian)

38. www.sworld.com.ua/konferm1/306.pdf

39. cpo.stu.cn.ua/Oksana/harch_himia_lekcii/730.html

40. lib.chmnu.edu.ua/pdf/posibnuku/210/3.pdf


Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 S. Yu. Makogonenko, V. I. Baranov, O. I. Terek

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.