THE EFFECT OF PROINFLAMMATORY ENZYMES INHIBITORS ON FUNCTIONING OF L-ARGININE/NO SYSTEM AND IMMUNOLOGICAL PARAMETERS IN BLOOD OF RATS UNDER THE EXPERIMENTAL COLITIS
DOI: http://dx.doi.org/10.30970/sbi.1001.463
Abstract
It was shown that under the experimental colitis in rats’ blood significant changes in the functioning of the system of L-arginine / NO and immunological disorders took place. A correction of violations was carried out by pro-inflammatory enzyme inhibitors, such as selective inhibitor COX-2 and dual selective blocker COX-2/5-LOX. The activity of NO-synthase and arginase, the content of L-arginine and nitrite-anion in animals’ blood were determined using Stat Fax; concentration of circulating immune complexes, such as IL-1β and TNF-α, were measured by the immuno-enzymatic methods. A significant increase in CIC, IL-1β and TNF-α was demonstrated. This can contributes to an increase in vascular tone, deterioration of microcirculation, hypoxia and development of oxidative stress. It was shown that NO hyperproduction is desreased at using inhibitors of pro-inflammatory enzymes and the effect of investigated medication had a positive correcting impact on the organism, reducing the overproduction of NO and normalizing immunological parameters of blood. More pronounced effect was observed in the case of using a dual inhibitor of COX-2/5-LOX.
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