PIGMENTS AND ULTRASTRUCTURAL PECULIARIES OF CELL ORGANELLES OF FERN POLYSTICHUM ACULEATUM (L.) ROTH. AT DIFFERENT STAGES OF DEVELOPMENT

M. M. Shcherbatiuk, L. M. Babenko, O. V. Vasheka, I. V. Kosakivska


DOI: http://dx.doi.org/10.30970/sbi.1102.526

Abstract


Qualitative and quantitative content of photosynthetic pigments in fronds of P aculeatum sporophyte at different stages of ontogeny was determined. The maximum content of photosynthetic pigments was registered at the stages of spore fructification and winter vegetation. A specific feature of fronds of P. aculeatum sporophyte is a relatively high content of carotenoids. At the stage of sori formation (May–June) mesophyllous cells of P. aculeatum fronds contained chloroplasts of rounded and elongated lenticular shape. Rounded chloroplasts stroma and grana, formed with small number of thylakoids are relatively electron-loose. The lenticular chloroplasts with electron-dense stroma and grana fill their volume. These chloroplasts contain large starch grains and occasionally plastoglobules. The ultrastructural differentiation of chloroplasts was not associated with certain anatomical areas of fronds. In May, chloroplasts in mesophyllous cells are located close to the plasmalemma. The ultrastructural ordering of the inner membrane system of parenchymal chloroplasts of P. aculeatum fronds were affected by seasonal temperature variation and changes in the lighting conditions. At the stage of autumn vegetation in the chloroplasts of mesophyllous cells there are sometimes formed neoplasms. A partial destruction of their internal membrane system and emergence of numerous plastoglobules were observed. The number of starch granules in the plastids was significantly reduced. Parenchyma cells of P. aculeatum rootstock had a significant amount of amyloplasts both in spring, at the stage of sori formation, and at the stage of autumn vegetation. These organelles were densely filled with starch grains while a developed internal membrane system was practically missing. The absence of significant changes in their ultrastructure is primarily associated with the optimal conditions for P. aculeatum (area of the ferns in Acad. O. V. Fomin Botanical Garden of Taras Shevchenko Kyiv National University).


Keywords


Polystichum aculeatum (L.) Roth., ontogeny, pigments, chloroplasts, ultrastructure

References


1. Athanasiou K., Dyson B.C., Webster R.E, Johnson G.N. Dynamic acclimation of photosynthesis increases plant fitness in changing environments. Plant Physiol, 2010; 152(1): 366-373.
https://doi.org/10.1104/pp.109.149351
PMid:19939944 PMCid:PMC2799370

2. Austin J.R., Frost E., Vidi P.-A., Kessler F., Staehelin L.A. Plastoglobules are lipoprotein subcompartments of the chloroplast that are permanently coupled to thylakoid membranes and contain biosynthetic enzymes. Plant Cell, 2006; 18(7): 1693-1703.
https://doi.org/10.1105/tpc.105.039859
PMid:16731586 PMCid:PMC1488921

3. Babenko L.M., Shcherbatiuk M.M., Kosakivska I.V. Lipoxygenase activity and rhizomes ultrastructure of vegetative and generative shoots of Equisetum arvense L. Studia Biologica, 2015; 9(1): 153-162. (In Ukrainian)
https://doi.org/10.30970/sbi.0901.405

4. Bailey S., Horton P., Walters R.G. Acclimation of Arabidopsis thaliana to the light environment: the relationship between photosynthetic function and chloroplast composition. Planta, 2004; 218(5): 793-802.
https://doi.org/10.1007/s00425-003-1158-5
PMid:14648116

5. Bréhélin C., Kessler F., Van Wijk K. J. Plastoglobules: versatile lipoprotein particles in plastids. Trends Plant Sci, 2007; 12(6): 260-266.
https://doi.org/10.1016/j.tplants.2007.04.003
PMid:17499005

6. Buchov N.G. Dynamic light regulation of photosynthesis. Russian Journal of Plant Physio­logy, 2004; 51(6): 742-753.
https://doi.org/10.1023/B:RUPP.0000047822.66925.bf

7. De Groot G.A., Zuidema P.A., De Groot H. & During H.J. Variation in ploidy level and phenology can result in large and unexpected differences in demography and climatic sensitivity between closely related ferns. American Journal of Botany, 2012; 99(8): 1375-1387.
https://doi.org/10.3732/ajb.1100482
PMid:22859655

8. Evert R.F. Esau's plant anatomy. Hoboken, New Jersey: Wiley Interscience, 2007. 607 p.

9. Evert R.F., Eichhorn S.E., Raven P.H. Raven Biology of Plants. New York: W.H. Freeman and Co. Publishers, 2013. 880 p.
https://doi.org/10.1007/978-1-319-15626-8

10. Gago J., Coopman R.E., Cabrera H.M., Hermida C. et al. Photosynthesis limitations in three fern species. Physiologia Plantarum, 2013; 149(4): 599-611.
https://doi.org/10.1111/ppl.12073
PMid:23692357

11. Havaux M., Ponfils J.P., Lutz C., Nijgoi K.K. Photo-damage of the photosynthetic apparatus and its dependence on the leaf developmental stage in the NPQ1 Arabidopsis mutants deficient in the xanthophylls cycle enzyme violaxanthin de-epoxidase. Plant Physiology, 2000; 124(1): 272-284.
https://doi.org/10.1104/pp.124.1.273
PMid:10982442

12. Hietz P., Briones O. Photosynthesis, chlorophyll fluorescence and within-canopy distribution of epiphytic ferns in a Mexican cloud forest. Plant Biol, 2001; 3(3) 279-287.
https://doi.org/10.1055/s-2001-15198

13. Johnson G.N., Rumsey F.J., Headley A.D., Sheffield E. Adaptation to extreme low light in the fern Trichomanes speciosum. New Phytologist, 2000; 148(3): 423-431.
https://doi.org/10.1046/j.1469-8137.2000.00772.x

14. Kochubey S.M., Bondarenko O.Yu., Shevchenko V.V. Photosynthesis. V. 1. The structure and functional peculiarities of light phase of photosynthesis. Kiev: Logos, 2014. 384 p. (In Russian)

15. Kotukhov Yu.A. The Technique for seasonal observation of the ferns of the family Polypodiaceae R. Br. Bulletin of the Main Bot. Garden, 1974; 94: 10-18. (In Russian)

16. Leong T.Y., Anderson J.M. Adaptation of the thylakoid membranes of pea chloroplast to light intensities. I. Study on the distribution of chlorophyll-protein complexes. Photosynt. Res, 1984; 5(2): 105-115.
https://doi.org/10.1007/BF00028524
PMid:24458599

17. Lichtenthaler H. K., Ab A., Marek M. V., Kalina, J., Urban O. Differences in pigment composition, photosynthetic rates and chlorophyll fluorescence images of sun and shade leaves of four tree species. Plant Physiol. Biochem, 2007; 45: 577-588.
https://doi.org/10.1016/j.plaphy.2007.04.006
PMid:17587589

18. Lotova L.I. The morphology and anatomy of higher plant. Moscow: Editorial URSS, 2001. 528 p. (In Russian)

19. Nasrulhaq-Boyce A., Haji Mohamed M.A. Photosynthetic and respiratory characteristics of malayan sun and shade ferns. New Phytologist, 1987; 105(1): 81-88.
https://doi.org/10.1111/j.1469-8137.1987.tb00112.x

20. Nishida K., Kodama N., Yonemura S., Hanba Y.T. Rapid response of leaf photosynthesis in two fern species Pteridium aquilinum and Thelypteris dentata to changes in CO2 measured by tunable diode laser absorption spectroscopy. J. Plant Res, 2015; 128(5): 777-789.
https://doi.org/10.1007/s10265-015-0736-5
PMid:26038271 PMCid:PMC4550647

20. Öquist G., Huner N.P.A. Photosynthesis of overwintering evergreen plants. Annu. Rev. Plant Biol, 2003; 54: 329-355.

21. Roux J.P., Van Wyk A.E. Morphology and anatomy of the rhizome and frond in the African species of Polystichum (Pteropsida: Dryopteridaceae). Bothalia, 2000; 30(1): 57-68.
https://doi.org/10.4102/abc.v30i1.542

22. Shcherbatiuk M.M., Brykov V.O., Martyn G.G. The preparation of plant tissues for electron microscopy (theoretical and practical aspects). Kyiv: Talkom, 2015. 66 p. (In Ukrainian).

23. Spicher L., Kessler F. Unexpected roles of plastoglobules (plastid lipid droplets) in vitamin K1 and E metabolism. Curr. Opin. Plant Biol, 2015; 25: 123-129.
https://doi.org/10.1016/j.pbi.2015.05.005
PMid:26037391

24. Sytnikov D.M., Babenko L.M., Shcherbatiuk M.M. The photosynthetic pigments and ontogeny of Equisetum arvense L. Bulletin of the Odessa National University. Ser. Biology, 2013; 18(2): 50-60.
https://doi.org/10.18524/2077-1746.2013.2(31).44732

25. Syvash O.O., Mykhaylenko N.F., Zolotaryova O.K. Sugars as a key element in the regulation of metabolism of photosynthetic cells. Ukr. Bot. J, 2001; 58(1): 121-127. (In Ukrainian).

26. Taiz L., Zeiger E. Plant Physiology Sunderland, Massachusetts: Sinauer Associates, Inc., Publisher, 2003. 623 p.

27. Topchiy N.M., Sytnik S.K., Syvash O.O., Zolotareva O.K. The effect of additional red irradiation on the photosynthetic apparatus of Pisum sativum. Photosynthetica, 2005; 43(3): 451-456.
https://doi.org/10.1007/s11099-005-0072-4

28. Tosens T., Nishida K., Gago J. et al. The photosynthetic capacity in 35 ferns and fern allies: mesophyll CO2 diffusion as a key trait. New Phytologist, 2016; 209(4): 1576-90.
https://doi.org/10.1111/nph.13719
PMid:26508678

29. Valladares F. Light heterogeneity and plants: from ecophysiology to species coexistence and biodiversity. In: Progress in Botany. Berlin Heidelberg: Springer, 2003: 439-471.
https://doi.org/10.1007/978-3-642-55819-1_17

30. Vasco A., Moran R.C., Ambrose B.A. The evolution, morphology, and development of fern leaves. Front. Plant Sci, 2013, 4: 1-16.
https://doi.org/10.3389/fpls.2013.00345
PMid:24027574 PMCid:PMC3761161

31. Vasheka O.V. The some biological characteristics of ferns of genus Dryopteris Adans. introduced into the open ground in the Acad. O.V. Fomin Botanical Garden. Bulletin of the State Nikitin Botanical Garden, 2004; 89: 12-15. (In Ukrainian)

32. Vasheka O.V., Brayon O.V. Petiole anatomical structure of evergreen ferns of the family Dryopteridaceae Ching. Plant Introduction, 2000; (1): 68-70. (In Ukrainian)

33. Vasheka O.V., Bezsmertna О.O. Fern atlas of Ukrainian flora: monograph. Kyiv: Palyvoda A.V., 2012. 160 p. (In Ukrainian)

35. Wellburn A. The spectral determination of chlorophyll a and chlorophyll b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol, 1994; 144(3): 307-313.
https://doi.org/10.1016/S0176-1617(11)81192-2


Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Studia biologica

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.