MULTI-MARKER STUDY OF THE RESPONSE OF BIVALVE MOLLUSK UNIO TUMIDUS INDUCED BY THE COMPOUNDS OF TYPICAL MUNICIPAL EFFLUENTS
DOI: http://dx.doi.org/10.30970/sbi.1102.540
Abstract
The pharmaceutical and personal care products (PPCPs) became the most typical pollutants of the surface waters. The aim of this study was to evaluate the effects of the combine exposure to the common compounds of the municipal effluents, constituent of plastics bisphenol A (BPA) and popular medicine nifedipine (Nfd), on the model organism, a mussel Unio tumidus. Male U. tumidus were exposed for 14 days to the combination of Nfd (10 μM) and BPA (0.88 nM). The indices of oxidative stress, metabolic, immune and endocrine activity, metal balance, as well as the manifestations of toxicity were detected. The exposure caused the features known for the effect of Nfd: the activation of the oxidative stress response, particularly Cu, Zn-superoxide dismutase (by 6.0 times), glutathione and oxyradical levels, metabolic shift to the anaerobiosis due to the elevated level of lactate in the digestive gland. The manifestation of endocrine disruption typical for the BPA effect - the increased level of alkali-labile phosphates (vitellogenin-like proteins) in gonads was detected. The caspase-3 related apoptotic activity was suppressed; whereas the cathepsin D mediated proteolysis and immune response of phenoloxidase were up-regulated significantly. The signs of geno-, neuro- and cytotoxicity were detected. These results detect that the approximation of the experimental conditions to the environmentally realistic situation could assist the comprehensive forecasting of the effects of utilized PPCPs for the aquatic animals.
Keywords
Full Text:
PDFReferences
1. Aarab N., Lemaire-Gony S., Unruh E. et al. Preliminary study of responses in mussel (Mytilus edilus) exposed to bisphenol A, diallyl phthalate and tetrabromodiphenyl ether. Aquatic Toxicology, 2006; 78: S86−S92. | |
| |
2. Amin M.T., Alazba A.A., Manzoor U. A Review of Removal of Pollutants from Water/Wastewater Using Different Types of Nanomaterials. Advances in Materials Science and Engineering, 2014. | |
| |
3. Baršienė J., Rybakovas A. Cytogenetic damage in gill and gonad cells of bivalve mollusks. Ekologija, 2008; 54(4): 245-250. | |
| |
4. Canesi L., Borghi C., Ciacci C. et al. Bisphenol-A alters gene expression and functional parameters in molluscan hepatopancreas. Molecular and Cellular Endocrinolology, 2007; 276: 36-44. | |
| |
5. Escher B.I., Hackermüller J., Polte T. et al. From the exposome to mechanistic understanding of chemical-induced adverse effects. Environmental International, 2017; 99: 97-106. | |
| |
6. Falfushynska H., Gnatyshyna L., Horyn O., Stoliar O. Effects of n-TiO2 and bisphenol A on cellular stress indices of the freshwater bivalve Unio tumidus. Visnyk of the Lviv University. Biology Series, 2016. 73: 208-212. | |
| |
7. Falfushynska H., Gnatyshyna L., Horyn O. et al. Endocrine and cellular stress effects of zinc oxide nanoparticles and nifedipine in marsh frogs Pelophylax ridibundus. Aquatic Toxicology, 2017; 185: 171-182. | |
| |
8. Falfushynska H., Gnatyshyna L., Yurchak I. et al. Habitat pollution and thermal regime modify molecular stress responses to elevated temperature in freshwater mussels (Anodonta anatina: Unionidae). Science of the Total Environment, 2014; 500-501: 339-350. | |
| |
9. Falfushynska H., Gnatyshyna L., Yurchak I. et al. The effects of zinc nanooxide on cellular stress responses of the freshwater mussels Unio tumidus are modulated by elevated temperature and organic pollutants. Aquatic Toxicology, 2015; 162: 82-93. | |
| |
10. Falfushynska H.I., Gnatyshyna L.L., Osadchuk O.Y. et al. Diversity of the molecular responses to separate wastewater effluents in freshwater mussels. Comparative Biochemistry and Physiology, 2014; 164: 51-58. | |
| |
11. Fent K., Weston A.A., Caminada D. Ecotoxicology of human pharmaceuticals. Aquatic Toxicology, 2006; 76: 122-159. | |
| |
12. Hrubik J., Glisic B., Tubic A. et al. Toxicological and chemical investigation of untreated municipal wastewater: Fraction-and species-specific toxicity. Ecotoxicology and Environmental Safety, 2016; 127: 153-162. | |
| |
13. Kolpin D.W., Furlong E.T., Meyer M.T. et al. Pharmaceuticals, hormones, and other organic wastewater contaminants in U. S. streams, 999-2000: a national reconnaissance. Environmental Science & Technology. 2002; 36(6): 1202-1211. | |
| |
14. Lowry O.H., Rosebroungh H.J., Farr A.L., Randall R.J. Protein measurement with folin phenol reagent. Journal of Biological Chemistry 1951; 193: 265-275. | |
| |
15. Luna-Acosta A., Breitwieser M., Renault T., Thomas-Guyon H. Recent findings on phenoloxidases in bivalves. Marine Pollution Bulletin, 2017; 122(1-2): 5-16. | |
| |
16. Noyes P.D., Lema S.C. Forecasting the impacts of chemical pollution and climate change interactions on the health of wildlife. Current Zoology, 2015; 61(4): 669-689 | |
| |
17. Pandey A.K., Deshpande S.B. Bisphenol A depresses compound action potential of frog sciatic nerve in vitro involving Ca(2+)-dependent mechanisms. Neuroscience Letters, 2012; 517(2):128−132. | |
| |
18. Rhee J.S., Raisuddin S., Hwang D.S. et al. Differential expression of metallothionein (MT) gene by trace metals and endocrine-disrupting chemicals in the hermaphroditic mangrove killifish, Kryptolebias marmoratus. Ecotoxicology and Environmental Safety, 2009, 72(1): 206−212. | |
| |
19. Viarengo A., Lowe D., Bolognesi C. et al. The use of biomarkers in biomonitoring: a 2-tier approach assessing the level of pollutant-induced stress syndrome in sentinel organisms. Comparative Biochemistry and Physiology, 2007, 146C: 281-300. | |
| |
20. Wagner M., Oehlmann J. Endocrine disruptors in bottled mineral water: total estrogenic burden and migration from plastic bottles. Environmental Science and Pollution Research, 2009, 16: 278-286. |
Refbacks
- There are currently no refbacks.
Copyright (c) 2017 Studia biologica
This work is licensed under a Creative Commons Attribution 4.0 International License.