Na + , K + -ATPase AND Ca 2+ , Mg 2+ -ATPase ACTIVITY IN SPERMATOZOA OF INFERTILE MEN WITH DIFFERENT FORMS OF PATHOSPERMIA

O. I. Meskalo; R. V. Fafula; E. I. Lychkovskyj; Z. D. Vorobets

doi:10.30970/sbi.1102.524

Na⁺, K⁺-ATPase AND Ca²⁺, Mg²⁺-ATPase ACTIVITY IN SPERMATOZOA OF INFERTILE MEN WITH DIFFERENT FORMS OF PATHOSPERMIA

O. I. Meskalo, R. V. Fafula, E. I. Lychkovskyj, Z. D. Vorobets

DOI: http://dx.doi.org/10.30970/sbi.1102.524

Abstract

Ion-exchanging ATPases play an essential role in biology of spermatozoa, including their motility, hyperactivation, chemotaxis, acrosome reaction etc. The aim of present study was to analyze Na⁺, K⁺-ATPase and Ca²⁺, Mg²⁺-ATPase activities in spermatozoa of the infertile men with different forms of pathospermia and to explore a possible role that they may play in male infertility. A significant reduction in ouabain-sensitive Na⁺, K⁺-ATPase activity in sperm cells of infertile men with oligozoo-, asthenozoo-, oligoasthenozoo- and leucocytospermia was shown. The results show that asthenozoo-, oligoasthenozoo- and leucocytospermic patients have significantly impaired thapsigargin-sensitive and thapsigargin-insensitive Ca²⁺, Mg²⁺-ATPase activity compared to healthy men. However, Ca²⁺, Mg²⁺-ATPase activity has a tendency to increase in patients with oligozoospermia. The depressed ATPase activity in the infertile men could be due to a reduction in intracellular adenosine triphosphate level and damage of spermal membranes caused by lipid peroxidation products. The most significant decrease in Ca²⁺, Mg²⁺-ATPase activity was observed in patients with leucocytospermia which could be explained by an excessive formation of the reactive oxygen species by the leucocytes. It is suggested that a decrease in the ion-exchanging ATPase activity may damage sperm functions and be one of possible causes of male infertility.

Keywords

Na+, K+-ATPase, Ca2+, Mg2+-ATPase, male infertility, pathospermia

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