BIOCHEMICAL COMPONENTS OF NITROGEN BALANCE IN SOIL UNDER CRUDE OIL CONTAMINATION AND PHYTOREMEDIATION
DOI: http://dx.doi.org/10.30970/sbi.1802.763
Abstract
Introduction. Nitrogen availability in oil contaminated soil is adversely affected due to an increase in the inorganic carbon (C) and nitrogen (N) ratio, unfavorable changes in physical, chemical and biological properties of the soil. Phytoremediation of oil contaminated soil may have a positive effect on N cycling. Changes in soil enzyme activity, content of organic and mineral N in the soil can be used as indicators of nitrogen balance. This paper aims to identify some biochemical elements of N cycling in oil contaminated soil and the effect of plants in recovering N balance in the soil.
Materials and Methods. In this study artificially oil contaminated soil (50 mL of crude oil per 1 kg of soil) was remediated by plants Zea mays L. and Vicia faba var. minor. Soil samples were collected on the 10th day after oil pollution, on the 22nd day (seeds were sown), on the 65th day (30 days of plants’ growth), and on the 95th day (60 days of plants’ growth). Soil without oil and plant vegetation was used as control. We determined the nodulation ability of soil, activity of soil protease and urease, content of labile organic nitrogen and free amino acids accumulation in the soil.
Results. Our study revealed the absence of nodules on V. faba roots in oil contamination conditions. Protease activity was inhibited in oil contaminated soil compared to control. Under plant vegetation and oil contamination conditions, soil protease activity increased on the 65th day, and decreased on the 95th day compared to oil contaminated soil without plants. Amino acid concentration was significantly smaller for oil contaminated soil during the experiment than for soil without oil, but amino acid content was significantly greater for soil planted with Z. mays than for soil without plants. V. faba had a stimulating effect on amino acid accumulation only for uncontaminated soil compared to soil without plants. The results have shown that the urease activity decreased in oil contaminated soil during all experimental period. Results indicated that 22 days after oil pollution, the content of labile organic N decreased in oil contaminated soil, whereas on further stages of the experiment it was not significantly different compared to control. Significant reduction of labile N was revealed for oil contaminated soil with Z. mays plants on the 95th day and with V. faba plants on the 65th day compared to uncontaminated soil with plants.
Conclusions. Oil-contamination had a negative effect on all studied biochemical characteristics of soil: protease activity, urease activity, labile organic nitrogen, free amino acids accumulation. In oil contamination conditions the positive effect of Z. mays and V. faba plants was determined for protease activity on the 65th day, a positive effect of Z. mays plants on accumulation of free amino acids accumulation was observed on the 95th day. The shortage of labile N in oil contaminated soil increased during phytoremediation. Therefore application of Z. mays and V. faba plants for optimization of biochemical components of N balance in oil contaminated soil may be controversial and requires further exploration.
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Adedeji, J. A., Tetteh, E. K., Opoku Amankwa, M., Asante-Sackey, D., Ofori-Frimpong, S., Armah, E. K., Rathilal, S., Mohammadi, A. H., & Chetty, M. (2022). Microbial bioremediation and biodegradation of petroleum products - a mini review. Applied Sciences, 12(23), 12212. doi:10.3390/app122312212 Crossref ● Google Scholar | ||||
| ||||
Borowik, A., & Wyszkowska, J. (2018). Bioaugmentation of soil contaminated with diesel oil. Journal of Elementology, 23(4), 1161-1178. doi:10.5601/jelem.2018.23.1.1627 Crossref ● Google Scholar | ||||
| ||||
Bunio, L. V., Tsvilynjuk, O. M., Mykiyevych, І. M., Velychko, О. І., & Terek, О. І. (2010). Microflora activity of clued oil contaminated soil in rhizosphere of Carex hirta L. plants. Studia Biologica, 4(3), 55-62. doi:10.30970/sbi.0403.109 (In Ukrainian) Crossref ● Google Scholar | ||||
| ||||
Bunio, L. V., Tsvilynjuk, O. M., Karpyn O. L., & Terek, O. I. (2013). Enzymatic activity of oil-contaminated soil in the root zone of Carex hirta L. plants. Gruntoznavstvo, 14(3-4), 43-51. (In Ukrainian) Google Scholar | ||||
| ||||
Chaîneau, C. H., Yepremian, C., Vidalie, J. F., Ducreux, J., & Ballerini, D. (2003). Bioremediation of a crude oil-polluted soil: biodegradation, leaching and toxicity assessments. Water, Air, and Soil Pollution, 144(1/4), 419-440. doi:10.1023/a:1022935600698 Crossref ● Google Scholar | ||||
| ||||
Diab, A. E. (2008). Phytoremediation of oil contaminated desert soil using the rhizosphere effects. Global Journal of Environmental Research, 2(2), 66-73. Google Scholar | ||||
| ||||
Dovgajuk-Semenyuk, M. V., Veluchko, O. I. & Terek, O. I. (2014). Vmist osnovnykh elementiv zhyvlennia ta rist roslyn koniushyny luchnoi u naftozabrudnenomu grunti, pidzhyvlenomu bakterialnym dobryvom mikro-vital [The content of main nutrition elements and red clover plants growth in the oil polluted soil fueled by the Micro-Vital bacterial fertilizer]. Scientific Bulletin of UNFU, 24(9), 128-131. Retrieved from https://nv.nltu.edu.ua/Archive/2014/24_9/25.pdf (In Ukrainian) Google Scholar | ||||
| ||||
Dovgajuk-Semenuk, M. V., Veluchko, O. I., & Terek, O. I. (2015). The content of ammonium and nitrate nitrogen in the red clover plants under the influence of oil polluted soil and fertalization with phosphorus-potassium fertilizers. Scientific Issue Ternopil Volodymyr Hnatiuk National Pedagogical University. Series Biology, 1(62), 94-99. Retrieved from http://dspace.tnpu.edu.ua/bitstream/123456789/5665/1/Dovgauk.pdf (In Ukrainian) Google Scholar | ||||
| ||||
Dzhura, N. M., Moroz, O. M., Rusyn, I. B., Kulachkovsky, O. R., Tsvilуnyuk, O. M., & Terek, O. I. (2010). Influence of the fodder beans (Vicia faba var. minor) on the Nitrogen metabolism of the microbe associations in the oil-polluted soil. Gruntoznavstvo, 11(3-4), 105-112. Retrieved from http://ussj.cv.ua/2010_t11_3-4/Dzyura.pdf (In Ukrainian) Google Scholar | ||||
| ||||
Dzhura, N. M., Romanyuk, O. I., Gonsyor, J., Tsvilynuk, O. М., & Terek, O. I. (2006). Using plants for restoration of the oil-cut soils. Ecology and Noosperology, 17(1-2), 55-60. Retrieved from http://www.uenj.cv.ua/17_tom-1_2/Djura_W.pdf (In Ukrainian) Google Scholar | ||||
| ||||
Elrys, A. S., Desoky, E.-S. M., Zhu, Q., Liu, L., Yun-xing, W., Wang, C., Shuirong, T., Yanzheng, W., Meng, L., Zhang, J., & Müller, C. (2024). Climate controls on nitrate dynamics and gross nitrogen cycling response to nitrogen deposition in global forest soils. Science of The Total Environment, 920, 171006. doi:10.1016/j.scitotenv.2024.171006 Crossref ● PubMed ● Google Scholar | ||||
| ||||
Fonseca-López, D., Vivas Quila, N. J., & Balaguera-López, H. E. (2020). Techniques applied in agricultural research to quantify nitrogen fixation: a systematic review. Ciencia y Tecnología Agropecuaria, 21(1), 32-50. doi:10.21930/rcta.vol21_num1_art:1342 Crossref ● Google Scholar | ||||
| ||||
Geisseler, D., & Horwath, W. R. (2008). Regulation of extracellular protease activity in soil in response to different sources and concentrations of nitrogen and carbon. Soil Biology and Biochemistry, 40(12), 3040-3048. doi:10.1016/j.soilbio.2008.09.001 Crossref ● Google Scholar | ||||
| ||||
Gianfreda, L., & Rao, M. A. (Eds.). (2014). Enzymes in agricultural sciences. Foster City: OMICS Group eBooks. Retrieved from http://uploads.worldlibrary.net/uploads/pdf/20150423221243enzymes_june14.pdf Google Scholar | ||||
| ||||
Greenfield, L. M., Puissant, J., & Jones, D. L. (2021). Synthesis of methods used to assess soil protease activity. Soil Biology and Biochemistry, 158, 108277. doi:10.1016/j.soilbio.2021.108277 Crossref ● Google Scholar | ||||
| ||||
Hamkalo, Z. G. (2008). Ekolohichna yakist gruntu [Ecological quality of soil]. Publishing Center Ivan Franko National University of Lviv. (In Ukrainian) Google Scholar | ||||
| ||||
Henry, H. A. L., & Jefferies, R. L. (2002). Free amino acid, ammonium and nitrate concentrations in soil solutions of a grazed coastal marsh in relation to plant growth. Plant, Cell & Environment, 25(5), 665-675. doi:10.1046/j.1365-3040.2002.00849.x Crossref ● Google Scholar | ||||
| ||||
Hrytsaenko, Z. M. , Hrytsaenko, A. O., & Karpenko, V. P. (2003). Metody biolohichnykh ta ahrokhimichnykh doslidzhen roslyn i gruntiv [Methods of biological and agrochemical research of plants and soils]. Kyiv: Nichlava. (In Ukrainian) Google Scholar | ||||
| ||||
Igiehon, B. C., Babalola, O. O., & Hassen, A. I. (2024). Rhizosphere competence and applications of plant growth-promoting rhizobacteria in food production - a review. Scientific African, 23, e02081. doi:10.1016/j.sciaf.2024.e02081 Crossref ● Google Scholar | ||||
| ||||
Jämtgård, S., Näsholm, T., & Huss-Danell, K. (2008). Characteristics of amino acid uptake in barley. Plant and Soil, 302(1-2), 221-231. doi:10.1007/s11104-007-9473-4 Crossref ● Google Scholar | ||||
| ||||
John, R. C., Itah, A. Y., Essien, J. P., & Ikpe, D. I. (2011). Fate of nitrogen-fixing bacteria in crude oil contaminated wetland ultisol. Bulletin of Environmental Contamination and Toxicology, 87(3), 343-353. doi:10.1007/s00128-011-0320-1 Crossref ● PubMed ● PMC ● Google Scholar | ||||
| ||||
John, R. C., Ntino, E. S., & Itah, A. Y. (2016). Impact of crude oil on soil nitrogen dynamics and uptake by legumes grown in wetland ultisol of the Niger Delta, Nigeria. Journal of Environmental Protection, 07(04), 507-515. doi:10.4236/jep.2016.74046 Crossref ● Google Scholar | ||||
| ||||
Jones, D. L., Healey, J. R., Willett, V. B., Farrar, J. F., & Hodge, A. (2005). Dissolved organic nitrogen uptake by plants - an important N uptake pathway? Soil Biology and Biochemistry, 37(3), 413-423. doi:10.1016/j.soilbio.2004.08.008 Crossref ● Google Scholar | ||||
| ||||
Kumar, G., Bhatt, P., & Lal, S. (2021). Phytoremediation: a synergistic interaction between plants and microbes for removal of petroleum hydrocarbons. In M. L. Larramendy & S. Soloneski (Eds.), Soil contamination - threats and sustainable solutions (pp. 1-14). London: IntechOpen. doi:10.5772/intechopen.93764 Crossref ● Google Scholar | ||||
| ||||
Li, H., Zhang, Y., Zhang, C. G., & Chen, G. X. (2005). Effect of petroleum-containing wastewater irrigation on bacterial diversities and enzymatic activities in a paddy soil irrigation area. Journal of Environmental Quality, 34(3), 1073-1080. doi:10.2134/jeq2004.0438 Crossref ● Google Scholar | ||||
| ||||
Li, Y., Wang, C., Wu, J., Zhang, Y., Li, Q., Liu, S., & Gao, Y. (2023). The effects of localized plant-soil-microbe interactions on soil nitrogen cycle in maize rhizosphere soil under long-term fertilizers. Agronomy, 13(8), 2114. doi.org/10.3390/agronomy13082114 Crossref ● Google Scholar | ||||
| ||||
Liu, H., Wu, M., Gao, H., Gao, J., & Wang, S. (2023). Application of 15N tracing and bioinformatics for estimating microbial-mediated nitrogen cycle processes in oil-contaminated soils. Environmental Research, 217, 114799. doi:10.1016/j.envres.2022.114799 Crossref ● PubMed ● Google Scholar | ||||
| ||||
Marinescu, M., Dumitru, M., Lăcătuşu, A., & Marinescu, M. (2011). Evolution of Maize biomass in a crude oil polluted soil according to the treatment applied. In Scientific papers. Series A. Agronomy (Vol. 54. pp. 287-292). Bucharest: University of Agronomic Sciences and Veterinary Medicine Google Scholar | ||||
| ||||
McDonald, J. H. (2014). Handbook of biological statistics. Baltimore, Maryland: Sparky House Publishing. Retrieved from http://www.biostathandbook.com/index.html Google Scholar | ||||
| ||||
Pan, W., Tang, S., Zhou, J., Liu, M., Xu, M., Kuzyakov, Y., Ma, Q., & Wu, L. (2022). Plant-microbial competition for amino acids depends on soil acidity and the microbial community. Plant and Soil, 475(1-2), 457-471. doi:10.1007/s11104-022-05381-w Crossref ● Google Scholar | ||||
| ||||
Rennenberg, H., Dannenmann, M., Gessler, A., Kreuzwieser, J., Simon, J., & Papen, H. (2009). Nitrogen balance in forest soils: nutritional limitation of plants under climate change stresses. Plant Biology, 11(s1), 4-23. doi:10.1111/j.1438-8677.2009.00241.x Crossref ● PubMed ● Google Scholar | ||||
| ||||
Sui, X., Wang, X., Li, Y., & Ji, H. (2021). Remediation of petroleum-contaminated soils with microbial and microbial combined methods: advances, mechanisms, and challenges. Sustainability, 13(16), 9267. doi:10.3390/su13169267 Crossref ● Google Scholar | ||||
| ||||
Vavrek, M. C., Colgan III, W., & Campbell, W. J. (2005). The role of plant-bacterial-fungal interaction in remediation of terrestrial oil spills. Retrieved from https://www.researchgate.net/publication/251503342_the_role_of_plant_bacterial_fungal_interaction_in_remediation_of_terrestrial_oil_spills Google Scholar | ||||
| ||||
Velychko, O. I. (2014). Rol bilkiv u adaptatsii roslyn koniushyny luchnoi, inokulovanoi Rhizobium leguminosarum bv. trifolii, do umov naftozabrudnenoho hruntu [Role of proteins in the adaptation of red clover plants inoculated with Rhizobium leguminosarum bv. trifolii to the condition of oil polluted soil]. Scientific Issue Ternopil Volodymyr Hnatiuk National Pedagogical University. Series Biology, 3(60), 58-61. Retrieved from http://dspace.tnpu.edu.ua/bitstream/123456789/4600/4/Velychko.pdf (In Ukrainian) Google Scholar | ||||
| ||||
Vinolas, L. C., Healey, J. R., & Jones, D. L. (2001). Kinetics of soil microbial uptake of free amino acids. Biology and Fertility of Soils, 33(1), 67-74. doi:10.1007/s003740000291 Crossref ● Google Scholar | ||||
| ||||
Wang, G., Ren, Y., Bai, X., Su, Y., & Han, J. (2022). Contributions of beneficial microorganisms in soil remediation and quality improvement of medicinal plants. Plants, 11(23), 3200. doi:10.3390/plants11233200 Crossref ● PubMed ● PMC ● Google Scholar | ||||
| ||||
Wyszkowska, J., & Kucharski, J. (2000). Biochemical properties of soil contaminated by petrol. Polish Journal of Environmental Studies, 9(6), 479-486. Retrieved from https://www.pjoes.com/pdf-87337-21196?filename=21196.pdf Google Scholar | ||||
| ||||
Yu, Y., Zhang, Y., Zhao, N., Guo, J., Xu, W., Ma, M., & Li, X. (2020). Remediation of crude oil-polluted soil by the bacterial rhizosphere community of Suaeda salsa revealed by 16S rRNA genes. International Journal of Environmental Research and Public Health, 17(5), 1471. doi:10.3390/ijerph17051471 Crossref ● PubMed ● PMC ● Google Scholar |
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