GROWTH OF VARIOUS STRAINS OF SULFATE-REDUCING BACTERIA FROM HUMAN LARGE INTESTINE

I. V. Kushkevych, O. M. Moroz


DOI: http://dx.doi.org/10.30970/sbi.0603.243

Abstract


New strains of sulfate-reducing bacteria from human intestine are obtained. These bacteria are vibrio-like (strains SRB Vib-1, SRB Vib-2, SRB Vib-3) and short rod-like (strains SRB Rod-4 and SRB Rod-5) forms. The growth of these strains of bacteria, usage of sulfates by bacteria and their production of hydrogen sulfide are investigated. The strains of sulfate-reducing bacteria SRB Vib-1, SRB Vib-2, SRB Vib-3 more intensively accumulate biomass, compared with strains SRB Rod-4 і SRB Rod-5. The strains SRB Vib-1 and SRB Vib-2 grow the most intensively. Accumulation of bacterial biomass (3.8 g/l) is the highest on the third day of cultivation, after that the stationary growth phase began. The strain of bacteria SRB Vib-3 accumulates biomass 2.89 g/l on the third day of bacteria cultivation. Maximal biomass of SRB Rod-4 and SRB Rod-5 bacteria strains ranged from 2.59 to 3.25 g/l on the eighth day of cultivation. The obtained sulfate-reducing bacteria intensively use sulfate ions and produce hydrogen sulfide. The strains SRB Vib-1, SRB Vib-2, SRB Vib-3 produce from 2.99 mM to 3.12 mM of hydrogen sulfide. The rod-shaped strains of sulfate-reducing bacteria use sulfate ions and produce hydrogen sulfide less intensely in the presence of sulfates in the medium.


Keywords


sulfate-reducing bacteria, Desulfovibrio, Desulfomicrobium, sulfates, hydrogen sulfide, bowel diseases, ulcerative colitis, intestinal microbiocenosis

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