GROWTH OF VARIOUS STRAINS OF SULFATE-REDUCING BACTERIA FROM HUMAN LARGE INTESTINE
DOI: http://dx.doi.org/10.30970/sbi.0603.243
Abstract
New strains of sulfate-reducing bacteria from human intestine are obtained. These bacteria are vibrio-like (strains SRB Vib-1, SRB Vib-2, SRB Vib-3) and short rod-like (strains SRB Rod-4 and SRB Rod-5) forms. The growth of these strains of bacteria, usage of sulfates by bacteria and their production of hydrogen sulfide are investigated. The strains of sulfate-reducing bacteria SRB Vib-1, SRB Vib-2, SRB Vib-3 more intensively accumulate biomass, compared with strains SRB Rod-4 і SRB Rod-5. The strains SRB Vib-1 and SRB Vib-2 grow the most intensively. Accumulation of bacterial biomass (3.8 g/l) is the highest on the third day of cultivation, after that the stationary growth phase began. The strain of bacteria SRB Vib-3 accumulates biomass 2.89 g/l on the third day of bacteria cultivation. Maximal biomass of SRB Rod-4 and SRB Rod-5 bacteria strains ranged from 2.59 to 3.25 g/l on the eighth day of cultivation. The obtained sulfate-reducing bacteria intensively use sulfate ions and produce hydrogen sulfide. The strains SRB Vib-1, SRB Vib-2, SRB Vib-3 produce from 2.99 mM to 3.12 mM of hydrogen sulfide. The rod-shaped strains of sulfate-reducing bacteria use sulfate ions and produce hydrogen sulfide less intensely in the presence of sulfates in the medium.
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1. Babko A.K., P'yatnitsky І.V. Quantitative analysis. K.: High School, 1974; 243 p. | |
| |
2. Barton L.L., Hamilton W.A. Sulphate-reducing Bacteria. Environmental and Engineered. Cambridge University Press, 2007; 553 p. | |
| |
3. Cummings J.H., Macfarlane G.T., Macfarlane S. Intestinal Bacteria and Ulcerative Colitis. Curr. Issues Intest. Microbiol, 2003; 4: 9-20. | |
| |
4. Egorov N.S. A guide to practical training in microbiology. Pract. allowance. Moscow: Mosk. University, 1983; 215 p. | |
| |
5. Gibson G.R., Cummings J.H., Macfarlane G.T. Growth and activities of sulphate-reducing bacteria in gut contents of health subjects and patients with ulcerative colitis. FEMS Microbiol. Ecol, 1991; 86: 103-112. | |
| |
6. Gibson G.R., Macfarlane G.T., Cummings J.H. Sulphate-reducing bacteria and hydrogen metabolism in the human large intestine. Gut, 1993; 34: 437-439. | |
| |
7. Gibson G.R., Macfarlane S., Macfarlane G.T. Metabolic interactions involving sulphate-reducing and methanogenic bacteria in the human large intestine. FEMS Microbiol. Ecol, 1993; 12: 117-125. | |
| |
8. Holt J., Krieg R., Snit P. et al. Bergey's Determinant of bacteria. Moscow: Publishing House of Mir, 1997; 1: 426 p. | |
| |
9. Isakova O.P., Tarasevych Yu.Yu., Yuzjuk Yu.I. Processing and visualization of data with the physical experiments by usage Origin package. Moscow: Book House «LIBKOM», 2009; 136 p. | |
| |
10. Karavayko G.I., Kuznetsov S.I., Golomzik A.I. A role of microorganisms in a leaching of metals from ores. Moscow: Science, 1972; 248 p. | |
| |
11. Kreshkov A.I. Fundamentals of analytical chemistry. Theoretical bases. Book First, ed. Fourth, revised, Moscow: Chemistry, 1976; 472 p. | |
| |
12. Kushkevych I.V. Sulfate-reducing bacteria of the human intestine. I. Dissimilatory sulfate reduction. Біологічні студії / Studia Biologica, 2012; 6(1): 149-180. | |
| |
13. Kushkevych I.V. Sulfate-reducing bacteria of the human intestine. II. The role in the diseases development. Біологічні студії / Studia Biologica, 2012; 6(2): 221-250. | |
| |
14. Lakin G.F. Biometry. Moscow: High School, 1990; 352 p. | |
| |
15. Langendijk P.S., Kulik E.M., Sandmeier H. et al. Isolation of Desulfomicrobium orale sp. nov. and Desulfovibrio strain NY682, oral sulfate-reducing bacteria involved in human periodontal disease. J. Syst. Evol. Microbiol, 2001; 51(3): 1035-1044. | |
| |
16. Loubinoux J., Bronowicji J.-P., Pereira I.A. et al. Sulphate-reducing bacteria in human feces and their association with inflammatory diseases. FEMS Microbiol. Ecol, 2002; 40: 107-112. | |
| |
17. Loubinoux J., Mory F., Pereira I.A. et al. Bacteremia caused by a strain of Desulfovibrio related to the provisionally named Desulfovibrio fairfieldensis. J. Clin. Microbiol, 2000; 38: 931-934. | |
| |
18. Macfarlane S., Dillon J.F. Microbial biofilms in the human gastrointestinal tract. J. Appl. Microbiol, 2007; 102: 1187-1196. | |
| |
19. Macfarlane S., Hopkins M.J., Macfarlane G.T. Bacterial growth and metabolism on surfaces in the large intestine. Microb. Ecol. Health Dis, 2000; 2: 64-72. | |
| |
20. Moore W.E.C., Johnson J.L., Holdeman L.V. Emendation of Bacteroidaceae and Butyrivibrio and descriptions of Desulfomonas gen. nov. and ten new species of the genera Desulfomonas, Butyrivibrio, Eubacterium, Clostridium and Ruminicoccus. Int. J. Syst. Bacteriol, 1976; 26: 238-252. | |
| |
21. Pitcher M.C., Cummings J.H. Hydrogen sulphide: a bacterial toxin in ulcerative colitis? Gut, 1996; 39: 1-4. | |
| |
22. Postgate J.R. The sulfate-reducing bacteria. 2nd ed. Cambridge: Cambridge Univ. Press, 1984; 199 p. | |
| |
23. Reynolds E.S. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol, 1963; 17: 208-212. | |
| |
24. Rowan F.E., Docherty N.G., Coffey J.C. et al. Sulphate-reducing bacteria and hydrogen sulphide in the aetiology of ulcerative colitis. British Journal of Surgery, 2009; 96: 151-158. | |
| |
25. Sekirov I., Russell S.L., Antunes L.C.M., Finlay B.B. Gut Microbiota in Health and Disease. Physiol Rev, 2010; 90: 859-904. | |
| |
26. Uikly B. Electron microscopy for beginners. Moscow: Publishing House of Mir, 1975; 314 p. | |
| |
27. Zinkevich V.V., Beech I.B. Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa. FEMS Microbiol. Ecol, 2000; 34: 147-155. | |
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