CEREBROLYSIN® INFLUENCES IN Sod- AND sws-DEPENDENT NEURODEGENERATIVE MODELS OF DROSOPHILA MELANOGASTER
DOI: http://dx.doi.org/10.30970/sbi.1702.708
Abstract
Background. The incidence of human neurodegenerative disorders increases continuously as the human population ages. To date, these diseases remain incurable and require complex experimental approaches using tractable models to study the degeneration mechanisms and potential drug intervention regimens. In the current work, we assessed the impact of the neuroprotective drug Сerebrolysin on these neurodegenerative processes in Drosophila Sod1 and swiss cheese (sws) mutants.
Materials and Methods. The experiments were conducted using a D. melanogaster Sod1- and sws-dependent neurodegeneration model. Сerebrolysin (3 μL/mL) was added for larvae feeding. In order to evaluate Сerebrolysin influence, several tests were performed: locomotor activity assay, lifespan, size of brain tissue degeneration zones and sensitivity to prooxidant exposion.
Results. Dietary supplementation with Сerebrolysin extended the lifespan of all flies under normal circumstances. The drug treatment also reduced the sensitivity of mutant flies to pro-oxidant effects. Moreover, treatment with Сerebrolysin partially diminished the size of degeneration zones in the brain tissue of sws1 mutant flies, without any notable effects on locomotor ability.
Conclusions. The data obtained confirm the moderate neuroprotective and/or antioxidant action of Сerebrolysinagainst neurodegenerative processes under different genetic backgrounds.
Keywords
neurodegeneration, Сerebrolysin, lifespan, behavior, Cu-Zn superoxide dismutase Sod1, swiss cheese genes
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