I. V. Panchuk, G. L. Antonyak



The mechanism of aflatoxin B1 influence on the cells of small intestine mucosa and the possibility of correction and prevention of metabolic disorders in animals using
“Vitakorm-BSR-Forte” phytopreparation was investigated. Intragastrial administration of aflatoxin B1 (15 µg/kg body weight daily, for 14 days) to albino rats resulted in chan­ges in the activity of mucosal NO-synthase, which mediate the process of synthesis of nitrogen oxide (NO) – an endogenous regulator of cellular functions. Changes in L-arginine concentration in the plasma of blood related to the activity of NO-synthases. At the same time, the aflatoxin B1 stimulating effect on the activities of antioxidant system enzymes and processes of lipid peroxidation in the cells of small intestine mucosa was observed. These results suggest that induction of formation of free radicals and initia­ting lipid peroxidation reactions, leading to a decline in antioxidant protection may represent one of the links in the pathogenic mechanisms of aflatoxin action. The administration of “Vitakorm-BSR-Forte” preparation in the conditions of aflatoxin action inhi­bited development of the oxidative stress and alleviated the NO-mediated disturbances in cellular metabolism, caused by the influence of aflatoxin. Consequently, “Vitakorm-BSR-Forte” preparation can exert positive effects in prevention and treatment of mycotoxicosis.


aflatoxin B1, NO-synthase, nitrites, lipid peroxidation, antioxidant system


1. Aleinikova T.L., Rubtsova G.V. Guide to practical training in biochemistry. M.: Higher School, 1988. 239 p. (Іn Russian)

2. Alpsoy L., Yalvac M.E. Key roles of vitamins A, C, and E in aflatoxin B1-induced oxidative stress. Vitam. Horm, 2011; 86: 287-305

3. Antonyak H.L., Kalynets-Mamchur Z.I., Dudka I.O. et al. The Ecology of Fungi. Lviv: Ivan Franko National University of Lviv, 2013. 600 р. (Іn Ukrainian)

4. Antonyak H.L., Fedyakov R.O., Koval N.K. Effect of aflatoxin B1 on the processes of lipid peroxidation and antioxidant system in red blood cells and hepatocytes of rats. Bulletin of ONU, 2011; 16 (6): 5-11. (Іn Ukrainian)

5. Antonyak H.L., Olijnyk Ch., Koval N. et al. Effects of aflatoxin b1 on lipid peroxidation and activities of antioxidant enzymes in rat organs and erythrocytes. Bulletin of Lviv University. Biology Series. Lviv, 2015; 69: 41-48.

6. Dede S., Deger Y., Kahraman T. et al. Effects of X-ray radiation of oxidation products of nitric oxide in rabbits treated with antioxidant compound. Turk. J. Biochem, 2009; 34 (1): 15-18.

7. Dubinina E.E., Salnikova L.A., Efimova L.F. Activity and isoenzyme spectrum of superoxide dismutase of human red blood cells and plasma Lab. Case, 1983; 10: 30-33. (In Russian)

8. El-Bahr S.M. Effect of curcumin on hepatic antioxidant enzymes activities and gene expressions in rats intoxicated with aflatoxin B1. Phytother Res, 2015. P. 134-40.

9. Fang C., Stavrou E., Schmairer A. et al. Angiotensin 1-7 and Mas decrease thrombosis in Bdkrb2−/− mice by increasing NO and prostacyclin to reduce platelet spreading and glycoprotein VI activation. Blood, 2013; 121(15): 3023-3032.
PMid:23386129 PMCid:PMC3624945

10. Grimm E.A. Immunology comes full circle in melanoma while specific immunity is unleashed to eliminate metastatic disease, inflammatory products of innate immunity promote resistance. Crit. Rev. Oncog, 2016; 21(1-2): 57-63.
PMid:27481002 PMCid:PMC5796770

11. Golovchak C.M., Antonyak H.L., Lysik I.A. Effect of aflatoxin B1 and drug of "Vitakorm-BSR-Forte" on the NO-synthase activity in rats' leukocytes. The Animal Biology, 2012; 14 (1-2): 596-600. (Іn Ukrainian)

12. Halliwell B., Gutteridge J.M.C. Free radicals in biology and medicine. Oxford: Uniwersity Press, 2007. 851 p.

13. Korobeinikova E.N. Modification of the LPO determination in the reaction with TBA. Lab. Case, 1989; 7: 8-10. (In Russian)

14. Koroljuk M.A., Ivanova I.G., Mayorova I.G. et al. The method for determining of the catalase activity. Lab. Сase, 1988; 1: 16-18. (Іn Russian)

15. Lapach S.I., Hubenko A.V., Babich P.P. Statistical methods in biomedical research using Excel. K.: Morion, 2000. 319 p. (In Russian)

16. Lowry O.H., Rosebrough N.J., Farr A.L. et al. Protein determination with the Folin phenol reagent. J. Biol. Chem, 1951; 193: 265-275.

17. Magierowski M., Magierowska K., Kwiecien S. et al. Gaseous mediators nitric oxide and hydrogen sulfide in the mechanism of gastrointestinal integrity, protection and ulcer healing. Molecules, 2015; 20: 9099-9123.
PMid:25996214 PMCid:PMC6272495

18. Monson M.S., Coulombe R.A., Reed K.M. et al. Aflatoxicosis: lessons from toxicity and responses to aflatoxin B1 in poultry. Agriculture, 2015; 5: 742-777.

19. Shamro N.R., Panasiuk N.B., Sklyarov O.J. Effect of vitamin C on the activities of NO-synthases and lipid peroxidation processes under conditions of blocking COX-2, iNOS or administration of L-arginine in experimental ulcerogenic colitis in rats. Medical Chemistry, 2011; 13 (1): 25-30. (In Ukrainian)

20. Sybirna N.O., Burda V.A., Chajka J.P. Methods for research of blood system: Textbook. Lviv: Publishing center of LNU after Ivan Franko, 2005. 100 p. (In Ukrainian)

21. Valko M., Leibfritz D., Moncol J. et al. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol, 2007; 39 (1): 44-84.

22. Wakita M., Kotani N., Yamaga T. et al. Nitrous oxide directly inhibits action potential-dependent neurotransmission from single presynaptic boutons adhering to rat hippocampal CA3 neurons. Brain Res. Bull, 2015; 118: 34-45.

23. Yunus A.W., Razzazi-Fazeli E., Bohm J. Aflatoxin B(1) in affecting broiler's performance, immunity, and gastrointestinal tract: a review of history and contemporary issues. Toxins (Basel), 2011; 566-590.
PMid:22069726 PMCid:PMC3202846

24. Zaccaria M., Ludovici M., Sanzani S.M. et al. Menadione-Induced oxidative stress re-shapes the oxylipin profile of Aspergillus flavus and its lifestyle. Toxins, 2015; 7: 4315-4329.
PMid:26512693 PMCid:PMC4626736


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