FATTY ACID COMPOSITION OF DESULFUROMONAS ACETOXIDANS IMB-7384 CELL UNDER THE INFLUENCE OF FERRIC CITRATE

O. Maslovska, S. Hnatush, A. Halushka


DOI: http://dx.doi.org/10.30970/sbi.0803.371

Abstract


Desulfuromonas acetoxidans ІМV В-7384 bacteria are perspective in the development of biotechnologies for remediation of wastewater from transitional metal ions. Investigation of defense mechanisms of D. acetoxidans IMV B-7384 cells from stress conditions, in particular from increasing concentrations of heavy metals is important for understanding pathways of regulation mechanisms of bacterial metabolism in the process of remediation of wastewater by bacteria. Changes in fatty acid composition of lipids are considered as one of possible ways of protection of bacterial cells under stress conditions. Addition of ferric citrate to the growth medium of D. acetoxidans ІМВ В-7384 bacteria caused changes in fatty acids composition compared to the control. Under the addition of ferric citrate in different concentrations the decrease of content of saturated fatty acids with odd carbon number was observed. The increase of content of saturated fatty acids with even carbon number, fatty acids with cyclopropane ring and branched fatty acids was observed under the influence of different concentrations of metal salt. Index of unsaturation fatty acids decreased under these conditions. The rate of cis-trans conversation of unsaturated fatty acids of D. acetoxidans ІМВ В-7384 cells did not increase under the influence of the ferric citrate.


Keywords


Desulfuromonas acetoxidans ІМV В-7384, ferric citrate, fatty acids

References


1. Bligh E., Dyer W. A rapid method for total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 1959; 37: 911-917.
https://doi.org/10.1139/y59-099

2. Golovchak N., Tarnovs'ka A., Kocjumbas G., Sanagurs'kij D. Lipid peroxidation in living organisms Lviv: Ivan Franko National University of Lviv, 2012. 250 p. (In Ukrainian)

3. Gudz S., Hnatush S., Moroz O. et al. Certificate of deposit strain of bacteria Desulfuromonas acetoxidans Ya-2006 in the Depository of Institute of Microbiology and Virology. Zabolotny NAS of Ukraine on granting registration number IMV B-7384 of 10 April 2013.

4. Guerzoni M., Lanciotti R., Cocconcelli P. Alteration in cellular fatty acid composition as a response to salt, acid, oxidative and thermal stresses in Lactobacillus helveticus. Microbiology, 2001; 147: 2255-2264.
https://doi.org/10.1099/00221287-147-8-2255
PMid:11496002

5. Heipieper H.,, Diefenbach R., Kewelon H. Conversion of cis unsaturated fatty acids to trans, a possible mechanism for the protection of phenol-degrading Pseudomonas putida P8 from substrate toxicity. Applied and Environmental Microbiology, 1992; 58 (6): 1847-1852.

6. Heipieper H., Meinhardt F., Segura A. The cis-trans isomerase of unsaturated fatty acids in Pseudomonas and Vibrio: biochemistry, molecular biology and physiological function of a unique stress adaptive mechanism. FEMS Microbiology Letters, 2003; 229: 1-7.
https://doi.org/10.1016/S0378-1097(03)00792-4

7. Heipieper H., Meulenbeld G., Oirschot Q. et al. Effect of environmental factors on the trans/cis ratio of unsaturated fatty acids in Pseudomonas putida S12. Applied and Environmental Microbiology, 1996; 62 (8): 2773-2777.

8. Kaneda T. Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiologycal Reviews, 1991; 55 (2): 288-302.

9. Lakin G. Biometrics. Moskva: Vysshaja shkola, 1990. 352 p. (In Russian)

10. Law J. Biosynthesis of cyclopropane rings. Accounts of Chemical Research, 1971; 4(6): 199-203.
https://doi.org/10.1021/ar50042a002

11. Lovely D., Giovannoni S., White D. Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals. Archives of Microbiology, 1993; 159: 336-344.
https://doi.org/10.1007/BF00290916

12. Lovely D., Holmes D., Nevin K. Dissimilatory Fe (III) and Mn (IV) reduction. Advances in Microbial Physiology, 2011; 59: 1-100.

13. Lovley D., Ueki T., Zhang T. Geobacter: the microbe electric's physiology, ecology, and practical applications. Advances in Microbial Physiology, 2004; 49: 219-286.
https://doi.org/10.1016/S0065-2911(04)49005-5

14. Lushchak V., Bagnyukova T., Lushchak O. Indices of oxidative stress. 1. TBA-reactive substrates and carbonylproteins. Ukrainian Biochemical Journal, 2004; 76 (3): 136-141. (In Ukrainian)

15. Magnuson K., Jackowski S., Rock C. et al. Regulation of fatty acid biosynthesis in Escherichia coli. Microbiologycal Reviews, 1993; 57 (3): 522-542.

16. Mannisto M., Puhakka J. Temperature- and growth-phase-regulated changes in lipid fatty acid structures of psychrotolerant groundwater Proteobacteria. Archives of Microbiology, 2001; 177: 41-46.
https://doi.org/10.1007/s00203-001-0356-4
PMid:11797043

17. Markowicz A., Plociniczak T., Piotrowska-Seget Z. Response of bacteria to heavy metals measured as changes in FAME profiles. Polish Journal of Environmental Studies, 2010; 19 (5): 957-965.

18. Maslovska O., Hnatush S. The intensity of lipid peroxidation and parameters of antioxidative defence system of Desulfuromonas acetoxidans IMV B-7384 under the influence of ferric (III) citrate. Visnyk of the Lviv University. Series Biology, 2014; 64: 270-278. (In Ukrainian)

19. Postgate J. The sulfate-reducing bacteria. Cambridge: Cambridge University Press, 1984. 208 p.

20. Tang X., Feng H., Chen W. Metabolic engineering for enhanced fatty acids synthesis in Saccharomyces cerevisiae. Metabolic Engineering, 2013; 16: 95-102.
https://doi.org/10.1016/j.ymben.2013.01.003
PMid:23353549

21. Vasyliv O., Bilyy O., Ferensovich Ja., Hnatush S. Electric current generation by sulfur-reducing bacteria in microbial-anode fuel cell. Proceedings of the SPIE, 2012; 8472: 84720Z-1-7.
https://doi.org/10.1117/12.925211

22. Vasyliv O. The influence of 3d-type transition metals on physiological and biochemical properties of Desulfuromonas acetoxidans bacteria. Dissertation Abstract of the Candidate of Biological Sciences. Kyiv, 2013: 20 р. (In Ukrainian)

23. Zhang E., Xu W., Diao G. et al. Electricity generation from acetate and glucose by sedimentary bacterium attached to elecrode in microbial-anode fuel cells. Journal of Power Sourses, 2006; 161: 820-825.
https://doi.org/10.1016/j.jpowsour.2006.05.004


Refbacks

  • There are currently no refbacks.


Copyright (c) 2014 Studia biologica

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.