MORPHOMETRIC CHARACTERISTICS OF RAT ADRENAL AND KIDNEY AT PRE-SLAUGHTER STRESS UNDER USING OF BIOLOGICALLY ACTIVE SUBSTANCES
DOI: http://dx.doi.org/10.30970/sbi.0802.354
Abstract
We have carried out studies of morphometric parameters of rat adrenal glands and kidney under stress conditions using biologically active substances of plant and animal origin: spleen, Echinacea and Chinese lemon extracts, sprouted grains. Animals which were not fed with immunomodulators and anti-stress compounds developed histological features of acute adrenal lesions characterized by thickening of gland capsule, reduction of glomerular zone, disorganized cellular structure, nuclear dystrophy, hyperchromy and pyknosis, resulting in increased their functional activity. Pathological changes in renal proximal tubules have been observed. It resulted in vacuolization of tubular epithelial cells and presence of desquamated epithelium in a tubular duct. Addition of spleen extract to food for five days before decapitation improved morphological state of adrenal gland and kidney suggesting anti-stress properties of polyamines present in spleen extract. These results obtained in model experiments on rats can be used in study of agricultural animals to prevent the effect of pre-slaughter animal stress on the quality of meat product.
Keywords
Full Text:
PDF (Українська)References
1. Bedanova I. P., Chloupek P., Vosmerova J. et al. Time Course Changes in selected biochemical stress indices in broilers exposed to short-term noise. Acta Vet, Brno, 2010; 79: 35-40. | |
| |
2. Chen Y. T., Sun C. K., Lin Y. C. et al. Adipose-derived mesenchymal stem cell protects kidneys against ischemia-reperfusion injury through suppressing oxidative stress and inflammatory reaction. J. Transl. Med, 2011; 9(1): 51-57. | |
| |
3. Glass L. Synchronization and rhythmic processes in physiology. Nature (Gr. Brit.), 2001; 410 (6825): 277-284. | |
| |
4. Grabovskyi S. S. Natural origin immunomodulators influence on cellular immunity indices and cortisol level in rats blood at pre-slaughter stress. Studia Biologica, 2014; 8(1): 93-102. (In Ukrainian) | |
| |
5. Grabovskyi S. S. Natural origin biologically active substances influence on laboratory animals under stress. Proceedings SWorld. Ivanovo: MARKOVA AD, 2013; 3(44): 13-15. (In Russian) | |
| |
6. Kachur І. V. Functional and morphological changes in adrenal glands and pituitary-thyroid system with traumatic stress. Abstract. dis ... cand. bіol. sciences. Kyiv, 2003. 20 р. (In Ukrainian) | |
| |
7. Lange C., Togel F., Ittrich H. et al. Westenfelder C: Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats. Kidney Int, 2005; 68:1613-1617. | |
| |
8. Morosanova M. A., Plotnikov E. Y., Pevzner I. B. et al. Kidney cell death in inflammation: The role of oxidative stress and mitochondria. Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 2014; 8.1: 103-110. | |
| |
9. Official Journal of the European Union L276/33. DIRECTIVE 2010/63/EU OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC. 20.10.2010. | |
| |
10. Pishak V. P., Huralyuk V. M. Effect of stress on the morphological organization of the adrenal glands at different times of day. Bukovinian Medical Bulletin, 2005; 9 (3): 135-137. (In Ukrainian) | |
| |
11. Rubio M., Rojas H., Rojas S., Ibarra V. Bienestar y productividad en parvadas de pollo de engorda tratadas con un modulador alostático durante la administración de vacunas. Memorias de la XXXVII Convención Nacional ANECA, del 02 al 06 mayo del 2012, Puerto Vallarta, Jalisco. México. 2012. | |
| |
12. Sergienko L. Y., Malova N. H., Heraschenko G. V. et al. Histostructural characteristics of functional status and reaction on stress of adrenal glands of stressed mother descendants. Probl. Endocrine. Pathological, 2004; 2: 69-74. (In Ukrainian) | |
| |
13. Servín R. J., Quintana L. J., Casaubon H. M. Assessment of Two Female Broiler Rearing Systems (Traditional and Modern) and Their Repercussion on Wellbeing, Corticosterone Concentration, Lesions in Adrenal Glands and Productive Parameters. International Journal of Poultry Science, 2013, 12.(6): 353-357. | |
| |
14. Togel F., Weiss K., Yang Y. et al. Westenfelder C: Vasculotropic,paracrine actions of infused mesenchymal stem cells are important tothe recovery from acute kidney injury. Am. J. Physiol. Renal Physiol, 2007; 292: 1626-1635. | |
| |
15. Vinogradov V. V. Stress: morph biology of adrenal cortex. Minsk: Belarussian Navuka, 1998. 319 p. (In Russian) | |
| |
16. Zikic D., Uscebrka G., Gledic D. et al. The influence of long term sound stress on histological structure of broiler's adrenal glands. Biotechnol. Anim. Husbandry, 2011; 27: 1613-1619. | |
Refbacks
- There are currently no refbacks.
Copyright (c) 2014 Studia biologica
This work is licensed under a Creative Commons Attribution 4.0 International License.