THE EFFECT OF PHOTOBIOMODULATION THERAPY ON SOME INDICES OF RATS’ BLOOD CELLS FUNCTIONAL STATE UNDER EXPERIMENTAL DIABETES MELLITUS

O. I. Karmash, M. Ya. Liuta, N. V. Yefimenko, N. O. Sybirna


DOI: http://dx.doi.org/10.30970/sbi.1503.659

Abstract


Background. Diabetes mellitus is a chronic endocrine-metabolic disease caused by an absolute or relative insulin deficiency. During diabetes, there are perfect conditions for the development of oxidative stress: the content of substrates for oxidation increases, the content of natural antioxidants decreases and the activity of antioxidant systems is suppressed. It is known that photobiomodulation therapy produce antioxidant and antihyperglycemic effects. Here we investigated its influence on blood system functioning.
Materials and Methods. The study was performed on male Wistar rats. Experimental diabetes mellitus was induced by the intraperitoneal injection of streptozotocin. Leukocyte formula was calculated using blood smears stained by Romanowsky–Giemsa. Catalase activity was determined spectrophotometrically. Affinity of hemoglobin to oxygen was evaluated by spectrophotometric method in Ivanov’s modification by drawing oxygenation curves. The protoporphyrin content in whole blood was measured by analyzing its fluorescence spectra. The content of NO2-, total and inducible NO synthase activity was determined spectrophotometrically.
Results. Under the action of photobiomodulation therapy on healthy animals, there was a shift of oxygenation curves to the left and a decrease of P50, whereas under irradiation of rats with diabetes, there was a shift of oxygenation curves to the right and increase in P50 compared to indices in nonirradiated animals. During diabetes, there was a decrease in protoporphyrin content compared to control, but there was a tendency to increase under photobiomodulation. Photobiomodulation therapy of rats with diabetes increased catalase activity in erythrocyte hemolysates. We revealed significant changes in leukocyte formula under photobiomodulation. The total NO synthase activity in leukocytes of rats with diabetes was higher compared to healthy animals, but decreased under the action of photobiomodulation. We found an increase in inducible NO synthase activity in leukocytes of rats with diabetes and in leukocytes of irradiated healthy animals. An increase in NO2- content in leukocytes of rats with diabetes was detected. Under photobiomodulation, NO2- content was significantly lower in rats with diabetes.
Conclusion. Photobiomodulation therapy produces a corrective action on blood system during diabetes, in particular, it improves oxygen release from hemoglobin and prevents hypoxia. Simultaneously with the increase in tissue oxygen saturation, a decrease in NO synthase activity and nitrite content along with an increase in catalase activity prevents the development of oxidative stress.


Keywords


diabetes mellitus, photobiomodulation therapy, hemoglobin, NO synthase

Full Text:

PDF

References


1. Benesch, R., Macduff, G., & Benesch, R. E. (1965). Determination of oxygen equilibria with a versatile new tonometer. Analytical Biochemistry, 11(1), 81-87.
Crossref ● PubMed ● Google Scholar

2. Dawson, J., & Knowles, R.G. (1998). A microtiter-plate assay of human NOS isoforms. Methods in molecular biology (Clifton, N.J.), 100, 237-242.
Crossref ● PubMed ● Google Scholar

3. De Freitas, L.F., & Hamblin, M.R. (2016). Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy. IEEE Journal of Selected Topics in Quantum Electronics, 22(3), 348-364.
Crossref ● PubMed ● PMC ● Google Scholar

4. Drel, V.R. (2010). Main mechanisms of the initiation and development of diabetic complications: the role of nitrative stress. Studia Biologica, 4(2), 141-158. [In Ukrainian]
Crossref ● Google Scholar

5. Gancheva, O.V., Kolesnyk, Y.M., & Vorodeeva, Y.I. (2016). Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia. Zaporozhye Medical Journal, 1(94), 35-39. [In Ukrainian]
Crossref ● Google Scholar

6. Handschin, C., Lin, J., Rhee, J., Peyer, A.K., Chin, S., Wu, P.H., Meyer, U.A., & Spiegelman, B.M. (2005). Nutritional regulation of hepatic heme biosynthesis and porphyria through PGC-1α. Cell, 122(4), 505-515.
Crossref ● PubMed ● Google Scholar

7. Ivanov, Y.G. (1975). Modification of the spectrophotometric method for determining the oxygen-dissociation curves of hemoglobin. Bulletin of Experimental Biology and Medicine, 80, 1399-1400. [In Russian]
Crossref ● Google Scholar

8. Karmash, O.I., Liuta, M.Y., Korobov, A.M., & Sybirna, N.O. (2020). Effect of photomodulation therapy on development of oxidative stress in blood leukocytes of rats with streptozocin-induced diabetes mellitus. Cytology and Genetics, 54(5), 456-464.
Crossref ● Google Scholar

9. Karmash, O.I., Lіuta, M.Y., Yefimenko, N.V., Korobov, A.M., & Sybirna, N.O. (2018). The influence of low-level light radiation of red spectrum diapason on glycemic profile and physicochemical characteristics of rat's erythrocytes in diabetes mellitus. Fiziolohichnyĭ Zhurnal, 64(6), 68–76. [In Ukrainian]
Crossref ● Google Scholar

10. Koroliuk, M.A., Ivanova, L.I., Maĭorova, I.G., & Tokarev, V.E. (1988). Metod opredeleniia aktivnosti katalazy [A method of determi­ning catalase activity]. Laboratornoe Delo, (1), 16-19. [In Russian]
PubMed ● Google Scholar

11. Kovalenko, O.V., & Kostenko, V.O. (2011). NO-dependent changes in lipid peroxidation processes and antioxidant protection in tissues of undermandibular salivary glands durinf recreation of traumatic sialoadenitis. Vìsnik problem bìologìї ì medicini, 4(90): 106-110. [In Ukrainian]
Google Scholar

12. Lim, C.K., Razzaque, M.A., Luo, J., & Farmer, P.B. (2000). Isolation and characterization of protoporphyrin glycoconjugates from rat Harderian gland by HPLC, capillary electrophoresis and HPLC/electrospray ionization MS. Biochemical Journal, 347(3), 757-761.
Crossref ● PubMed ● PMC ● Google Scholar

13. Lytvynets, Ye.A., & Hotsulyak, Ya.V. (2012). Peroxidation condition of lipids and antioxidant protection in the pathology of urine system and the expediency of using bioflavonoids in the complex treatment. Zdorov’e mužčiny, (1), 135-137. [In Ukrainian]
Google Scholar

14. Millana Fañanás, E., Todesca, S., Sicorello, A., Masino, L., Pompach, P., Magnani, F., Pastore, A., & Mattevi, A. (2020). On the mechanism of calcium-dependent activation of NADPH oxidase 5 (NOX5). The FEBS journal, 287(12), 2486-2503.
Crossref ● PubMed ● PMC ● Google Scholar

15. Miranda, K.M., Espey, M.G., & Wink, D.A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5(1), 62-71.
Crossref ● PubMed ● Google Scholar

16. Mokryi, V.Y., Ziablitsev, S.V., & Borys, R.M. (2015). Violations of the system of lipid peroxidation in type 2 diabetes mellitus (Literature Review). International Journal of Endocrinology, 7(71), 41-44. [In Ukrainian]
Crossref ● Google Scholar

17. Mungrue, I.N., Husain, M., & Stewart, D.J. (2002). The role of NOS in heart failure: lessons from murine genetic models. Heart failure reviews, 7(4), 407-422.
Crossref ● PubMed ● Google Scholar

18. Patel, S., Jose, A., & Mohiuddin, S.S. (2021). Physiology, Oxygen Transport And Carbon Dioxide Dissociation Curve. In StatPearls. StatPearls Publishing.
PubMed ● Google Scholar

19. Ribeiro, B.G., Alves, A.N., Dos Santos, L.A., Cantero, T.M., Fernandes, K.P., Dias, D., Bernardes, N., De Angelis, K., & Mesquita-Ferrari, R.A. (2016). Red and infrared low-level laser therapy prior to injury with or without administration after injury modulate oxidative stress during the muscle repair process. PloS One, 11(4), e0153618.
Crossref ● PubMed ● PMC ● Google Scholar

20. Sachar, M., Anderson, K.E., & Ma, X. (2015). Protoporphyrin IX: the Good, the Bad, and the Ugly. Journal of Pharmacology and Experimental Therapeutics, 356(2), 267-275.
Crossref ● PubMed ● PMC ● Google Scholar

21. Sassa, S., Granick, J.L., Granick, S., Kappas, A., & Levere, R.D. (1973). Studies in lead poiso­ning. I. Microanalysis of erythrocyte protoporphyrin levels by spectrophotometry in the detection of chronic lead intoxication in the subclinical range. Biochemical Medicine, 8(1), 135-148.
Crossref ● PubMed ● Google Scholar

22. Szablewski, L., & Sulima, A. (2017). The structural and functional changes of blood cells and molecular components in diabetes mellitus. Biological Chemistry, 398(4), 411-423.
Crossref ● PubMed ● Google Scholar

23. Voet, D., & Voet, J.G. (2011). Biochemistry (4th ed.). USA: John Wiley & Sons.

24. Xu, Y., Lin, Y., & Gao, S. (2015). Study on the selection of laser wavelengths in the intravascular low-level laser irradiation therapy. Lasers in Medical Science, 30(4), 1373-1376.
Crossref ● PubMed ● Google Scholar

25. Xu, Y., Lin, Y., Gao, S., & Shen, J. (2018). Study on mechanism of release oxygen by photo-excited hemoglobin in low-level laser therapy. Lasers in Medical Science, 33(1), 135-139.
Crossref ● PubMed ● Google Scholar


Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 O. I. Karmash, M. Ya. Liuta, N. V. Yefimenko, N. O. Sybirna

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.