СЕЛЕКЦІЯ МУТАНТІВ ДРІЖДЖІВ SCHEFFERSOMYCES STIPITIS ЗІ ЗМІНЕНОЮ ЗДАТНІСТЮ ДО АЛКОГОЛЬНОЇ ФЕРМЕНТАЦІЇ ГЛЮКОЗИ ТА КСИЛОЗИ МЕТОДОМ ІНСЕРЦІЙНОГО МУТАГЕНЕЗУ

Анотація

In our study we propose a strategy for the direct selection of the yeast strains producing higher amounts of ethanol. It was established that the small molecule 3–bromopyruvate specifically inhibits key enzymes of glycolysis: hexokinase, pyruvate kinase and pyruvate decarboxylase. The yeast cells resistant to 3–bromopyruvate should intensify glycolysis. It will stimulate synthesis of ethanol during the fermentation. Insertional mutant S. stipitis #4.6 is characterized by elevated amount of ethanol produced from glucose or xylose. Complementation mutant S. stipitis Comp_TMI1 produces similar amount of ethanol to a wild type strain.