BIOCHEMICAL AND HAEMATOLOGICAL CHANGES IN PERIPHERAL BLOOD OF RATS EXPOSED TO CHLORPYRINOS: PROTECTIVE EFFECT OF VITAMINS A AND E COMBINATION
DOI: http://dx.doi.org/10.30970/sbi.0903.448
Abstract
Chlorpyrifos is a highly toxic organophosphate compound. It is still among the most widely used insecticide, and the main mechanism of its toxicity is associated with inhibition of cholinesterases. A long with the anticholinesterase action, CPF may affect other biochemical mechanisms, particularly through disrupting pro- and antioxidant balance and inducing free-radical oxidative stress. We studied the action of A and E vitamins on the basic haematological and biochemical parameters of rat peripheral blood after 12 hours of a single chlorpyrifos intoxication. Exposure to 70 mg/kg chlorpyrifos caused a decrease in the total number of red blood cells (RBCs), platelets, and total haemoglobin content. We also observed a decrease in the acid haemolysis resistance of RBCs in peripheral blood of CPF-poisoned rats. Combined exposure to chlorpyrifos and vitamins A and E caused changes in haemolysis resistance of RBCs, approaching the control values. In addition, it was found that chlorpyrifos intoxication disrupt prooxidant-antioxidant balance as evidenced by the increase of lipid peroxidation products: lipid hydroperoxides and thiobarbituric acid reactive substances. However, administration of vitamins A and E during intoxication provided levelling effect on the formation of lipid peroxidation products. CPF intoxication caused an increase of the catalase activity, while superoxide dismutase, glutathione peroxidase and glutathione reductase activities and the content of reduced glutathione decreased. It was revealed that combination of vitamins A and E cause corrective effect at the platelets quantity, and lipid hydroperoxides thiobarbituric acid reactive substances amount of rat peripheral blood.
Keywords
Full Text:
PDFReferences
1. Amara I. Dimethoate-induced oxidative damage in erythrocytes of female adult rats: possible protective effect of vitamin E and selenium supplemented to diet. Toxicology and Industrial Health, 2011; 28(3): 222-237. | |
| |
2. Ambali S.F., Ayo J.O., Ojo S.A. et. al. Vitamin E protects Wistar rats from chlorpyrifos-induced increase in erythrocyte osmotic fragility. Food Chem.Toxicol, 2010; 48(12): 3477-80. | |
| |
3. Carlberg I. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J. Biol. Chem., 1975; 250: 5475-5480. | |
| |
4. Chelikani P., Fita I., Loewen P. Diversity of structures and properties among catalases. Cell. Mol. Life Sci, 2004; 61(2): 192-208. | |
| |
5. Dubinina E.E. The biological role of superoxide anion radical, and superoxide dismutase in body tissues. The Success. of Mod. Biol, 1989; 108(1): 3-18. (In Russian) | |
| |
6. Dudok K., Starykovych L., Rechytskyi A. et. al. Role of pyrrolopyrimidinedions derivatives in the regulation of haemoglobin physical and chemical characteristics and human blood antioxidant enzymes activity in vitro. Visnyk of Lviv University. Series Biology. 2012; 60: 126-136. (In Ukrainian). | |
| |
7. Gitel'zon I. I., Terskov I. A. The erythrogram as a method of clinical investigation of the blood. Krasnoyarsk: Scientific academy of USSR, Siberia department; 1959: 246. (In Russian) | |
| |
8. Grabovska S., Rosalovsky V., Salyha Y. Neurotoxic effects and other actual risks of chlorpyrifos. In: A. Sibirny, et al. (Ed.) Living organisms and bioanalytical aproaches for detoxification and monitoring of toxic compounds. Univ. of Rzeszow, 2015; 195-204. | |
| |
9. Grabovska S.V., Salyha Y.T. The effect of chronic intoxication with low doses of chlorpyrifos on the behavioral parameters of female rats. Fiziolohichnyi Zhurnal, 2014; 61(2): 94-101. (In Ukrainian) | |
| |
10. Hassani S., Sepand M.R., Jafari A. et al. Protective effects of curcumin and vitamin E against chlorpyrifos-induced lung oxidative damage. Hum Exp Toxicol, 2015; 34(6): 668-76. | |
| |
11. Hissin P.J., Hilf R.A. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Analyt. Biochem, 1976; 74: 214-226. | |
| |
12. Karpyshtshenko A. Medical laboratory technologies and diagnostics: Handbook. St. Petersburg: Intermedica, 1999. 656 p. (In Russian) | |
| |
13. Kharchenko O.A. Balan G.M., Bubalo N.N. Acute organophosphate poisoning: the main clinical syndromes and mechanisms of their formation (literature review and own research data).Modern Problems of Toxicology, 2013; 1(2): 17-31. (In Russian) | |
| |
14. Korobeynikova E.A. modification of lipid peroxidation products assessment in the reaction with thiobarbituric acid. Lab. Delo, 1989; 7: 8-9. (In Russian) | |
| |
15. Koroliuk M.A., Ivanova L.I., Mayorov I.G. et al. The method of determining the activity of catalase. Lab. Delo, 1988; 1: 16-18. (In Russian) | |
| |
16. Lowry O.H. Rosebrough N.J., Farr A.L. et al. Protein measurement with the Folin phenol reagent. J. Biol. Chem, 1951; 193(1): 265-75. | |
| |
17. Mashali A. Role of oxidative stress and apoptosis in acute organophosphorus intoxicated patients. JMRI, 2005; 26(3): 255-263. | |
| |
18. Moin V.A. simple and specific method for glutathione peroxidase activity assessment in erythrocytes. Lab. Delo, 1986; 12: 724-727. (In Russian) | |
| |
19. Rosalovsky V.P. Haematological indices of rats during the first hour after chlorpyrifos exposure. Biological Bulletin of Bohdan Chmelnitsky Melitopol State Pedagogical University, 2015; 1: 123-132. | |
| |
20. Salyha Y. Biological effects assessment of chlorpyrifos and some aspects of its neurotoxicity. Visnyk of Lviv University. Biology Series, 2010; 54: 3-14. | |
| |
21. Salyha Y.T. Antioxidant system indices in brain of rats intoxicated by chlorpyrifos. Studia Biologica, 2013; 7(3): 85-96. (In Ukrainian) | |
| |
22. Salyha, Yu.T. Chlorpyrifos leads to oxidative stress-induced death of hippocampal cells in vitro. Neurophysiology, 2013; 45 (3): 193-199. | |
| |
23. Sidhu I.P., Bhatti J.S., Bhatti G.K. Modulatory action of melatonin against chlorpyrifos induced hepatotoxicity in Wistar rats. Asian Journal of Multidisciplinary Studies, 2014; 2(9): 123-131. | |
| |
24. Spodniewska A. Barski D. Gizejewska A. Effect of enrofloxacin and chlorpyrifos on the levels of vitamins A and E in Wistar rats. Environ. Toxicol. Pharmacol, 2015; 40(2): 587-91. | |
| |
25. Vasilyeva E. M. Biochemical features of red blood cell. Effect of pathology. Biomedical Chemistry, 2005; 51(2) 118-126. (In Russian) | |
| |
26. Venkatesh S., Ramachandran A., Zachariah A. et al. Mitochondrial ATP synthase inhibition and nitric oxide are involved in muscle weakness that occurs in acute exposure of rats to monocrotophos. Toxicol. Mech. Methods, 2009; (3): 239-45. | |
| |
27. Vlizlo V.V., Fedoruk R.S., Ratych I.B. Laboratory methods of research in biology, animal husbandry and veterinary medicine. Lviv: Spolom, 2012. 764 c. (In Ukrainian) | |
| |
28. Vlizlo V.V., Salyha.Y.T. Problems of biological safety of pesticides in Ukraine. Bulletin of Agrarian Science, 2011; 1: 24-27. (In Ukrainian) | |
Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Studia biologica
This work is licensed under a Creative Commons Attribution 4.0 International License.