Biol. Stud. 2018: 12(3–4); 35–46 • DOI: https://doi.org/10.30970/sbi.1203.578

EFFECT OF CHROMIUM CITRATE ON LIPID COMPOSITION IN BLOOD PLASMA OF RATS WITH EXPERIMENTAL DIABETES

R. Iskra, O. Sushko, A. Pylypets, O. Slivinska

Abstract


The results of studying changes of lipid composition in blood plasma of rats with alloxan-induced diabetes under the condition of consumption of chromium citrate in the concentration of 0.1 and 0.2 µg/ml of water are presented. The experiment was conducted on 32 laboratory rats. The rats were divided in four groups: K1 – control group, K2 – control group with diabetes, R1 and R2 – experimental groups. Pure water (without any additives) was given to the rats of groups K1 and K2. Water with chromium citrate in concentration of 0.1 and 0.2 µg/ml was given to the animals from groups R1 and R2 for one month. Experimental diabetes mellitus was induced in the animals of groups K2, R1, and R2 by the intraperitoneal injection of 5% solution of alloxan monohydrate in concentration of 150 mg/kg of body weight. Total lipids content and their classes were determined in animals’ blood plasma.

It was found that an imbalance was present in the lipid profile and the phospholipid profile of animals with experimental diabetes mellitus. In particular, the content of total lipids and relative content of non-esterified cholesterol increased significantly, and there was a tendency of the content of triacylglycerols and unesterified fatty acids to be increased in the rats’ blood with experimental diabetes mellitus of K2 group. There was a shift in spectrum of different fractions when the content of phospholipids decreased in blood of rats with experimental diabetes mellitus.

The content of total lipids, esterified and non-esterified cholesterol significantly decreased and the content of triacylglycerols and unesterified fatty acids was significantly lower in animals of the experimental groups R1 and R2, but the content of phospholipids increased in the same groups. Changes in content of certain classes of lipids in blood of rats with alloxan diabetes affected by additives of citrate chromium are multidirectional and dose-dependent.

Keywords: rats, chromium citrate, blood, total lipids, phospholipids

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References


Cefalu W.T., Hu F.B. Role of chromium in human health and in diabetes. Diabetes Care, 2004; 27(11): 2741–2751.

[DOI: https://doi.org/10.2337/diacare.27.11.2741]

Czech M.P., Tencerova M., Pedersen D.J., Aouadi M. Insulin signalling mechanisms for triacylglycerol storage. Diabetologia, 2013; 56(5): 949–964.

[DOI: https://doi.org/10.1007/s00125-013-2869-1]

Espenshade P.J. SREBPs: sterol-regulated transcription factors. Journal of Cell Science, 2006; 119: 973–976.

[DOI: https://doi.org/10.1242/jcs02866]

Folch J., Lees M., Stanley G. A simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry, 1957; 226(1): 497–509.

Ganguly R., Sahu S., Ohanyan V., Haney R., Chavez R., Shah S., Yalamanchili S., Raman P. Oral chromium picolinate impedes hyperglycemia-induced atherosclerosis and inhibits pro­athe­rogenic protein TSP-1 expression in STZ-induced type 1 diabetic ApoE-/- mice. Scientific Reports, 2017;7: 45279.

[DOI: https://doi.org/10.1038/srep45279]

Ganguly R., Wen A.M., Myer A.B., Czech T., Sahu S., Steinmetz N.F., Raman P. Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro. Nanoscale, 2016; 8(12): 6542–6554.

[DOI: https://doi:org/10.1039/C6NR00398B]

Hua Y., Clark S., Ren J., Sreejayan N. Molecular mechanisms of chromium in alleviating insulin resistance. The Journal of Nutritional Biochemistry, 2012; 23(4): 313–319.

[DOI: https://doi.org/10.1016/j.jnutbio.2011.11.001]

Iskra R.Ja. The content of fatty acids in muscle and fatty tissues of pigs by the action of chromium chloride. Scientific Herald of Uzhgorod University. Biology, 2012; (32): 168–171. (In Ukrainian).

Iskra R.Ja. The peculiarities of operation of glutathione component of antioxidant protection and lipid metabolism in pregnant rat females under the action of chromium citrate. Studia Biologica, 2013; 7(1): 71–80.

[DOI: https://doi.org/10.30970/sbi.0701.276]

Iskra R.Ja., Vlizlo V.V., Fedoruk R.S., Antonyak H.L. Chromium in animal nutrition. K.: Agrarian science, 2014. 312 p. (In Ukrainian).

Jenkins T.C., McGuire M.A. Major advances in nutrition: impact on milk composition. Journal of Dairy Science, 2006; 89 (4): 1302–1310.

[DOI: https://doi.org/10.3168/jds.S0022-0302(06)72198-1]

Kates M. Techniques of lipidology. Amsterdam: Elsevier, 1986. 451 р.

Kegley E.B., Galloway D.L., Fakier T.M. Effect of dietary chromium-L-methionine on glucose metabolism of beef steers. Journal of Animal Science, 2000; 78(12): 3177–3183.

[DOI: https://doi.org/10.2527/2000.78123177x]

Kondrakhin I.P., Kurilov N.V., Malakhov A.G. Clinical laboratory diagnostics in veterinary scien­ce. M.: Agropromizdat, 1985. 287 p. (In Russian).

Kozachok M.M., Osodlo G.V., Kucz T.V. The role and place of essential phospholipids in the treatment of chronic diffuse liver diseases. Suchasna gastroenterologiya. 2006; 4(30): 95–101. (In Ukrainian).

Kuzyshyn O.V., Kovalishin N.V, Almashina H.V. Biochemistry of diabetes: 1. Theoretical part (review). Medical chemistry. 2010; 2: 74–115. (In Ukrainian).

Martin J., Wang Z.Q., Zhang X.H., Wachtel D., Volaufova J., Matthews D.E., Cefalu W.T. Chromium picolinate supplementation attenuates body weight gain and increases insulin sensitivity in subjects with type 2 diabetes. Diabetes Care, 2006; 29(8): 1826–1832.

[DOI: https://doi.org/10.2337/dc06-0254]

McNamara J.P., Valdez F. Adipose Tissue Metabolism and Production Responses to Calcium Propionate and Chromium Propionate. Journal of Dairy Science, 2005, 88: 2498–2507. [DOI:https://doi.org/10.3168/jds.S0022-0302(05)72927-1]

Refaie F.M., Esmat A.Y., Mohamed A.F., Aboul Nour W.H. Effect of chromium supplementation on the diabetes induced-oxidative stress in liver and brain of adult rats. Biometals, 2009; 22(6): 1075–1087.

[DOI: https://doi.org/10.1007/s10534-009-9258-8]

Steele N.C., Rosebrough R.W. Effect of trivalent chromium on hepatic lipogenesis by the turkey poult. Poultry Science, 1981; 60(3): 617–622.

[DOI: https://doi.org/10.3382/ps.0600617]

Sundaram B., Singhal K., Sandhir R. Ameliorating effect of chromium administration on hepatic glucose metabolism in streptozotocininduced experimental diabetes. Biofactors, 2012; 38(1): 59–68.

[DOI:https://doi.org/10.1002/biof.194]

Wilson B.E., Gondy A. Effects of chromium supplementation on fasting insulin levels and lipid parameters in healthy, non-obese young subjects. Diabetes Research and Clinical Practice, 1995; 28(3): 179–184.

[DOI: https://doi.org/10.1016/0168-8227(95)01097-W]




DOI: http://dx.doi.org/10.30970/sbi.1203.578

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