MICROBIOLOGICAL EXPERTISE OF INDOOR AIR AND BOOKS OF SCIENTIFIC LIBRARY OF IVAN FRANKO NATIONAL UNIVERSITY OF LVIV

G. V. Yavorska, I. S. Bilinska, S. O. Hnatush, G. S. Os’mak


DOI: http://dx.doi.org/10.30970/sbi.1001.447

Abstract


Microorganisms are able to damage wood, paper, paints and made from this materials chronicles, books etc. Indoor air microbiota of apartments of the Scientific Library of Ivan Franko National University of Lviv was analyzed. Air microbiota is a potential source of infecting of library founds. It was established that the amount of microorganisms in air was different dependently on season, temperature, humidity and isolation place (quantity at first layer level at middle at 2–7 folds high than at second layer level). 47 strains of fungi were isolated and identified. They make a threat for books in most probes of indoor air of library. These are fungi of Fusidium viride (4 %), Eidamella spinosa (2 %), Ulocladium sp. (2 %), Myxotrichum chartarum (2 %), Mortierella sp. (2 %), and representatives of genera Penicillium (62 %), Aspergillus (20 %), Mucor (2 %), Alternaria (4 %). In common, 89 % of isolated strains of micromycetes possess cellulase acti­vity, among them 12 % − possess high activity (completely decompose the parer in experiment). There are fungi of Eidamella spinosa (1 strain) and representatives of genera Penicillium (3 strains), Alternaria і Ulocladium (one each strain). Old prints with visible marks of microbe damages were investigated. They are kept at department of manuscript, old prints and rarity books named after F. P. Maksymenko of the library, because of presence of living microorganisms. On all books were discovered microorganisms, which were isolated in pure cultures and were identified as strains of fungi Verticillium album and representatives of genera Penicillium, Mucor, Aspergillus (species A. fumigatus and A. wentii), and also sporulating bacteria of Bacillus genus. To decreasing of contamination by microorganisms of infected books and to verification of action efficiency on micromycetes pure cultures was used disinfecting camera EK-21. Treatment of books and spores of fungi pure cultures in this camera (temperature 65±5 °C, humidity 60±5 %, formalin 100–150 ml) caused 100 % lethality of all present on it microorganisms. Thus, main important means of preventing of books biodamaging, which are maintenance in libraries, archives, museums, is conducting of systematic complex control of environment parameters and mycological monitoring of library apartments and objects of storage.


Keywords


air microbiota, book biodamaging, сellulase activity, micromycetes, disinfecting

References


1. Bilai V.I. Bases of general mycology. Kyiv: Vyshcha Shcola, 1989. 392 p. (In Russian)

2. Biodamaging of books and documents: practical work, M: Vysshaja Shkola, 1986. 80 p. (In Russian)

3. Bidzilia V.O., Mytkivska T.I. Ways of problem decision of fungi damages in the museums of Canada. Problems of maintenance, conservation and restoration of museum sights of history and culture, Kyiv: IPK PK, 1998. P. 126−142. (In Ukrainian)

4. Bondarenko I.O. Problem of maintenance of document founds: review of foreign sources.Bulletin of the Kharkiv State Academy of Culture, 2002; 10: 115-123. (In Ukrainian)

5. Vershynina E.G., Pokrovskaya Yu.V. Indoor micromycetes. Theory and practice of books storage in library. St. Petersburg, 1992: P. 113-119. (In Russian)

6. Voitovych V.A., Mokiva L.N. Biological corrosion. Moscow: Znanie, 1980. 50 p. (In Russian)

7. Volodina O.P., Zdanova N.M., Kondratiuk T.O. Documents defecting by mould fungi and measures on a labour protection during work with the damaged documents. Methodical recommendations. Kyiv: Institute of Microbiology and Virology of Ukraine Academy of Sciences, 2005. 48 p. (In Ukrainian)

8. Volodina O.P., Shevchenko L.O., Koval E.Z., Shurubura A.K. Microbiological state of documentary funds of Ukraine state archives. Proc. of 2nd Int. Scient. Pract. Conf. "Problems of maintenance, conservation and restoration of museum sights". Kyiv, 1999. P. 18-19. (In Ukrainian)

9. Gudz S.P., Hnatush S.O., Bilinska I.S. Microbiology: practical work, tests. Lviv: Lviv Ivan Franko National University Publishing Center, 2012. 228 p. (In Ukrainian)

10. Derkach M.P., Gumetskiy R.Y., Chaban M.Y. Course of variation statistics. Lviv: Lviv Ivan Franko National University Publishing Center, 1997. 207 p. (In Ukrainian)

11. Dovgaliuk V., Kondratiuk T., Rybchynska O., Riasna O. Temperature-humidity conditions and indoor air microbiota of archives and museums. Studies in Archived Business and Documentation Studying. Kyiv, 2000; 6: 103−106. (In Ukrainian)

12. Kozlova I.P., Radchenko O.S., Stepura L.G. et al. Geochemical activity of microorga­nisms and its applied aspects. Kyiv: Naukova Dumka, 2008. 528 p. (In Ukrainian)

13. Kondratiuk T.O., Nakonechna L.T., Artyshkova L.V. Ecological and systematical analysis of micromycetes from libraries and archives / Proc. of X Congress of Ukrainian Society of Microbiologists. Odesa: Astroprint, 2004. 216 p. (In Ukrainian)

14. Kondratiuk T.O. Prophylaxis of damages of museum collections by microscopical fungi / Problems of maintenance, conservation and restoration of museum sights of history and culture. Kyiv: IPK PK, 1998. P. 12−29. (In Ukrainian)

15. Litvinov M.A. Manual of the soil microscopic fungi. Leningrad: Nauka, 1967. 303 p. (In Russian)

16. Manturovskaya N.V., Sizova T.P., Saraeva V.M. Microbiological state of documents archives air. Proc. of 3rd All Union Conf. "Biodamages". Moscow, 1987; 1: 30-31. (In Russian)

17. Museum storage of artistic value: Practical manual. Moscow, 1995. 204 p. (In Russian)

18. Mokeeva V.L., Budarina E.E. Micromycetes of some archives of cinema and foto documents. Mycology and Phytopathology, 1991; 5: 404-410. (In Russian)

19. Novikova G. M. Documents maintenance in libraries and archives: Methodical recommendations, Kyiv, 2006. 56 p. (In Ukrainian)

20. Niuksha Ju. P. Biological damage of paper and books. SPb.: BAN, 1994. 232 p. (In Russian)

21. Onyshenko O.S. About the state and tasks of development the library-informative business in NAS of Ukraine. Library Bulletin, 2003; 5: 3-7. (In Ukrainian)

22. Os'mak G.S. Usage of propolis as antiseptic in restoration. Proc. of Int. Scient. Pract. Conf. "Restoration of museum sights in modern terms. Problems and ways of it decision". Kyiv, 1999. P. 18-19. (In Ukrainian)

23. Savchyn N., Kundys I., Bilinska I., Pavlyk Ju. The complex going to the question of library collections defense from biodamages / Proc. of 5th Int. Conf. "Problems of maintenance, conservation and restoration and expertise of museum sights". Kyiv, 2005. P. 270-271. (In Ukrainian)

24. Sergeeva L.E. A critical review of micromycetes species in libraries. Medical Mycology Advantages, 2007; 9: 72-73. (In Russian)

25. Subbota A.G., Novykova G.M. Mycological control of air as prophylaxis of biodamages of documents founds. Proc. of All Ukraine Conf. "Ukrainian Archives Studying: History, Modern State and Prospects". Kyiv, 1997; 2: 252-253. (In Ukrainian)

26. Parker T.A. Integrated Post Management for Libraries. Conference heldat the National Library of Austria "Preservation of Library Materials". Vienna, 1986. P. 103-123.

27. Bilai V.I., Kurbatskaya Z.A. Manual of the toxic forming micromycetes. Kyiv: Naukova Dumka, 1990. 236 р. (In Russian)

28. Milko A.A. Manual of the mucor fungi. Kyiv: Naukova Dumka, 1974. 302 р. (In Russian)

29. Zerov D.K. Manual of the fungi of Ukraine. Vol. III. Imperfect fungi. Kyiv: Naukova Dumka, 1971, 692 р. (In Ukrainian)

30. Bilaі V.I., Koval E. Z. Aspergilli. Kyiv: Naukova Dumka, 1988. 204 р. (In Russian)

31. Sharan L.O., Cirulnikova V.V., Pavliuchenko O.S. Hygiene and Sanitary: Course of Lections. Kyiv: NUFT, 2013. 29 p. (In Ukrainian)

32. http://undiasd.archives.gov.ua/doc/studiji/2000.pdf


Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Studia biologica

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.