CHANGES IN THE EXPOSURE OF CD11B ON THE SURFACE OF GRANULOCYTES, AFTER ACTIVATION OF TOLL-LIKE RECEPTORS IN CHILDREN WITH RECURRENT INFECTIONS
DOI: http://dx.doi.org/10.30970/sbi.1001.468
Abstract
The effect of activation of Toll-like receptors (TLR) on presentation of CD11b adhesion molecule at the granulocytes surface in children of different age with recurrent infections was investigated. Stimulation of TLR2, TLR4, TLR7/TLR8 by agonists led to a significant increase of the content of integrin CD11b in the granulocytes cytoplasmic membrane. The amount of CD11b after stimulation of TLR7/8, was lower in groups of children with recurrent infections, compared with matched-age control groups. The amount of integrin CD11b on granulocytes surface, following activation TLR, did not differ statistically between the studied and control groups of different ages. In addition, we analyzed the ligand-dependent content of CD11b in granulocytes surface membrane of each group. Thus, in the control groups, amount of CD11b did not differ statistically after incubation of blood cells with agonists of TLRs (LPS, R848 and PGN). Conversely, in groups of children with recurrent infections, levels of induced CD11b production were statistically distinctive depends on ligands and the lowest content of CD11b was obtained after incubation of blood cells with R848. Thus, exploring the influence of TLR activation on the amount of integrin CD11b on the granulocyte’s surface showed that the growth rate of CD11b after activation, independent of age. Groups of children with recurrent infections showed significantly lower amount of CD11b on granulocytes after activation of TLR7/8, compared with age-matched control groups. Such a decreased presentation of adhesion molecules at granulocyte’s surface in response to TLR7/8 stimulation by R848 can lead to preventing migration of granulocytes to the infection site and promote an increased sensitivity to viral infections and bacterial complications.
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