STRUCTURAL-FUNCTIONAL PECULIARITIES OF WATER FERN SALVINIA NATANS (L.) ALL.

L. M. Babenko, M. M. Shcherbatiuk, O. V. Polishchuk, I. V. Kosakivska


DOI: http://dx.doi.org/10.30970/sbi.1002.476

Abstract


The characteristics of photosynthetic pigments and efficient use of light energy are analyzed fon the example of chloroplast ultrastructure of the photosynthetic apparatus of floating and submerged fronds of annual heterosporous of water fern Salvinia natans (L.) All. in ontogenesis. It is established that at the stage of intensive growth chloroplasts floating fronds had a well-developed system of thylakoid membranes with many faces and contain a lot of starch grains. Submerged fronds shaped chloroplasts are smaller with little starch in the stroma. Destructive changes in chloroplast photosynthetic membranes of both frond types are observed at the stage of sporocarp formation. Particularly, decomposition of gran tylakoiod membranes, disturbances in inter-gran connections, reduction of starch grain number in stroma were revealed. In the chloroplast stroma of floating wai numerous plastoglobules were fouond. In chloroplasts submerged fronds fewer plastoglobules education were noted. In addition, a certain portion of popu­lation hloroplastoa in both types fronds retains intact structures.The photosynthetic pigment content of floating fronds was found to exceed that of submerged two times, and at some stages of ontogenesis – three times. At the stage of sporocarp formation, the chloroplast and carotinoid content in fronds of the both types decreased. Floating fronds are characterized by an effective action of the chloroplast photosynthetic electron transport chain, and are adapted to an intensive natural lighting on a water surface. Submerged fronds affected by lighting whose intensity fits with a maximum level of natural characteristics were found to be at stress. That was confirmed by a decrease in quantum efficiency ФСІІ (ΦPSII) and increase in dissipation of excessive light energy as heat in rise of the level of nonphotochemical quenching of chloroplast fluorescence (NPQ).


Keywords


Salvinia natans (L.) All., ultrastructure, pigments

References


1. Austin J.R., Frost E., Vidi P.-A., Kessler F., Staehelin L.A. Plastoglobules are lipoprotein subcompartments of the chloroplast that are permanently coupled to thylakoid membranes and contain biosynthetic enzymes. Plant Cell, 2006; 18: 1693-1703.
https://doi.org/10.1105/tpc.105.039859
PMid:16731586 PMCid:PMC1488921

2. Babenko L.M., Kosakivska I.V., Akimov Yu.A. et al. Еffect of temperature stresses on pigment content, lipoxygenase activity and cell ultrastructure of winter wheat seedlings. Genetics and Рlant Рhysiology, 2014; 4(1-2): 117-125.

3. Babenko L.M., Sheyko O.A., Kosakivska I.V. et al. Structural and functional characteristics of pteridophytes (Polypodiophyta) The Biulleten of Kharkiv National. Agrar. University (Ser. Biology), 2015; 1 (34): 80-103. (In Ukrainian)

4. Barthlott W., Wiersch S., Čolić Z., Koch K. Classification of trichome types within species of the water fern Salvinia, and ontogeny of the egg-beater trichomes. Botany, 2009; 87: 830-836.
https://doi.org/10.1139/B09-048

5. Bercu R. Anatomical features of the vegetative organs of Salvinia natans (L.) All. (Salviniaceae). USAMVBT Symposium. 5th section: Biology researchers with implications in agriculture, 2006: 321-324.

6. Bréhélin C., Kessler F., Van Wijk K. J. Plastoglobules: versatile lipoprote in particles in plastids. Trends Plant Sci, 2007; 12: 260-266.
https://doi.org/10.1016/j.tplants.2007.04.003
PMid:17499005

7. Brestic M., Zivcak M. PSII fluorescence techniques for measurement of drought and high temperature stress signal in crop plants: protocols and applications. In: Rout G.R., Das A.B. (Ed) Molecular Stress Physiology of Plants. Dordrecht: Springer, 2013, 87-131.
https://doi.org/10.1007/978-81-322-0807-5_4

8. Cholodny N.G. About metamorphosis of plastids in hairs of Salvinia natans submerged leaves. J. of Russian Bot. Soc, 1924; 7: 153-160. (In Russian)

9. Croxdale J.G. Salvinia leaves. I. Origin and early differentiation of floating and submerged leaves. Canadian Journal of Botany, 1978; 56: 1982-1991.
https://doi.org/10.1139/b78-237

10. Croxdale J.G. Salvinia leaves. III. Morphogenesis of the submerged leaf. Canadian Journal of Botany, 1981; 59: 2065-2072.
https://doi.org/10.1139/b81-268

11. Demming-Adams B. Characteristics and species-dependent employment of flexible versus sustained thermal dissipation and photoinhibition. In: Demmig-Adams B., Adams III W.W., Matoo A.K., (Ed.) Photoprotection, photoinhibition, gene regulation, and environment. Dordrecht: Springer, 2006: 39-48.
https://doi.org/10.1007/1-4020-3579-9_4

12. Dubyna D.В., Shelyag-Sosonko Yu.R., Zhmut O.I. et al. Danube biosphere reserve. Plants. Kyiv: Phytosociocentr, 2003. 210 р. (In Ukrainian)

13. Evert R.F. Esau's plant anatomy. 3rd edition. Hoboken, New Jersey: Wiley Interscience, 2007. 607 p.

14. Gałka A., Szmeja J. Phenology of the aquatic fern Salvinia natans (L.) All. in the Vistula Delta in the context of climate warming. Limnologica, 2013; 43: 100-105.
https://doi.org/10.1016/j.limno.2012.07.001

15. Genty B., Briantais J.M., Baker N.R., The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta, 1989; 990: 87-92.
https://doi.org/10.1016/S0304-4165(89)80016-9

16. Johnson, G. N., Young, A. J., Scholes, J. D., Horton, P. The dissipation of excess excitation energy in British plant species. Plant. Cell and Environment, 1993; 16: 673-679.
https://doi.org/10.1111/j.1365-3040.1993.tb00485.x

17. Klymenko O.M. Structural and functional aspects of Nuphar lutea heterophylly (L.) Smith. Kyiv: Thesis of PhD manuscript. 2014. 21 p (In Ukrainian)

18. Kochubey S.M., Bondarenko O.Yu., Shevchenko V.V. Photosynthesis.V. 1. The structure and functional peculiarities of light phase of photosynthesis. Kiev: Logos, 2014. 479 p. (In Russian)

19. Korneev D.Yu. Information capabilities of the method of chlorophyll fluorescence induction. Kiev: Alterpress, 2002. 188 p. (In Russian)

20. Lemon G.D., Posluszny U. Shoot morphology and organogenesis of the aquatic floating fern Salvinia molesta D. S. Mitchell, examined with the aid of laser scanning confocal microscopy. Internat. Jour. of Plant Sci, 1997; 158: 693-703.
https://doi.org/10.1086/297481

21. Maxwell K., Johnson G.N. Chlorophyll fluorescence - a practical guide. Jour. Exp Bot, 2000; 345(51): 659-68.
https://doi.org/10.1093/jexbot/51.345.659

22. Melis A. Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximize efficiency. Plant Science, 2009; 177: 272-280.
https://doi.org/10.1016/j.plantsci.2009.06.005

23. Nedukha O.M. Heterophylly in Plants. Kyiv: Alterpress, 2011. 192 p. (In Ukrainian)

24. Nedukha O.M. The ultrastructural characteristics of cells and pigments of Trapa natans L. floating and submerged leaves. Mod. Phytomorphol, 2011; 1: 81-84. (In Ukrainian)

25. Nedukha O.M. Anatomy of leaves and chloroplasts ultrastructure of some hydrophytes. Mod. Phytomorphol, 2015; 8: 162-168. (In Ukrainian)

26. Nekrasova G.F., Ronzhina D.A., Korobitsina E.B. The photosynthetic system formation during the growth of submerged, floating and overwater leaves of hydrophytes. Plant Physiol, 1998; 45: 539-548. (In Russian)

27. Nekrasova G.F., Ronzhina D.A., Maleva M.G., Pyankov V.I. The photosynthetic metabolism and activity of the carboxylation enzymes in overwater, floating and submerged leaves of hydrophytes. Plant Physiol, 2003; 50: 65-75. (In Russian)

28. Nielsen S.L. A comparison of aerial and submerged photosynthesis in some Danish amphibious plants. Aquat. Bot, 1993; 45: 27-40.
https://doi.org/10.1016/0304-3770(93)90050-7

29. Page C. Ecological strategies in fern evolution: a neopteridological overview. Rev. Palaeobot. Palynol, 2002; 119: 1-33.
https://doi.org/10.1016/S0034-6667(01)00127-0

30. Рringsheim N. Zur Morphologie der Salvinia natans. Jahrbuch für wissenschaftliche Botanik, 1863; 3: 484-541.

31. Rohacek K., Soukupova J., Bartak M. Chlorophyll fluorescence: a wonderful tool to study plant physiology and plant stress. In: Schoefs B. (Ed) Plant cell compartments - selected topics. Research Signpost: Trivandrum, 2008; 41-104.

32. Ross M. Mapping the world's pteridophyte diversity - sistematics and floras. In. Ross M. (Ed.) Pteridology in Perspective. - Kyiv: Rojal Botanic Gardens, 1996: 29-42.

33. Ryen F.J. Isolation and characterization of photosynthetically active cells from submerged and floating leaves of the aquatic macrophyte Potamogeton nodosus Poir. Plant Cell Phy­siol, 1985; 26: 309-315.

34. Sand-Jensen K., Frost-Christensen H. Plant growth and photosynthesis in the transition zone between land and stream. Aquat. Bot, 1999; 63: 23-35.
https://doi.org/10.1016/S0304-3770(98)00107-7

35. Shcherbatiuk M.M., Babenko L.M., Sheyko O.A., Kosakivska I.V. Microstructural features of water fern Salvinia natans (L.) All. organ surfaces. Modern Phytomorphology, 2015; 7: 129-133. (In Ukrainian)

36. Shcherbatiuk M.M., Brykov V.O., Martyn G.G. The preparation of plant tissues for electron microscopy (theoretical and practical aspects). Kyiv: Talkom, 2015. 62 p. (In Ukrainian)

37. Spicher L., Kessler F. Unexpected roles of plastoglobules (plastid lipid droplets) in vitamin K1 and E metabolism. Curr. Opin. Plant Biol, 2015; 25: 123-129.
https://doi.org/10.1016/j.pbi.2015.05.005
PMid:26037391

38. Wellburn A.The spectral determination of chlorophyll a and whlorophyll b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol, 1994; 144: 307-313.
https://doi.org/10.1016/S0176-1617(11)81192-2

39. Yang J.P., Dengler N.G., Horton R.F. Heterophylly in Ranunculus flabellaris: The effect of abscisic acid on leaf anatomy. Ann. Bot, 1987; 60: 117-125.
https://doi.org/10.1093/oxfordjournals.aob.a087427


Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Studia biologica

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.