SULFIDOGENIC ACTIVITY OF DESULFUROMUSA SP. SV30 UNDER THE INFLUENCE OF CHROMIUM, COPPER AND IRON COMPOUNDS
DOI: http://dx.doi.org/10.30970/sbi.1102.530
Abstract
Coal pit waste heap is an example of the multifactorial stress influence on organisms. The effect of heavy metals on the cell is the most toxic. Factor analysis of data for 29 variables, defined for gangues from coal pit waste heaps, was conducted. In the case of the analysis of red gangue four latent factors are revealed, it explains over 97 % total dispersion data. In the case of turfed by the mosses gangue as well as gangue without mosses analysis six latent factors were revealed, it explain about 87 and 86 % total dispersion data respectively. The ability of sulfur reducing bacteria Desulfuromusa sp. SV30, isolated from coal pits waste heaps of Chervonograd mining region, to utilize Cr (VI), Cu (II) and Fe (III) compounds as electron acceptors was studied. The influence of these compounds on growth and sulfidogenic activity of these bacteria was established. Desulfuromusa sp. СВ30 reduces 0.5–2 мМ of K2Cr2O7 and CuCl2, 1–10 мМ C6H5O7Fe as electron acceptors with varying intensity. 1 mМ of CuCl2 and C6H5O7Fe are completely reduced by bacteria. Desulfuromusa sp. SV30 incubation with heavy metals compounds (0.5 mM of K2Cr2O7, 1−2 mМ of CuCl or CuCl2, 1 mМ Fe2+ and 1−30 mМ Fe3+) causes bacteria metabolism intensification. Oxidized metals forms applying into the medium with sulfur at concentrations of 0.5 mМ (CuCl2) and 1 mМ leads to the higher biomass growth but sulfur reducing is strictly inhibited. At the presence of 0.5–1 мМ CuCl2 in medium 95.0−100.0 % of Cu2+ are bounded with HS- produced by the bacteria. After 1 mМ of FeCl2×4H2O applying into the medium the level of Fe2+ bounding with HS- equals 88.0 %. Indicated concentrations exceeding inhibited growth and sulfidogenic activity of Desulfuromusa sp. SV30. Highly effective application of Desulfuromusa sp. SV30 in technologies for purifying the environment from toxic sulfur- and metal-containing compounds is expected.
Keywords
Full Text:
PDF (Українська)References
1. Аbdulina D.R., Purish L.М., Аsaulenko L.G. et. al. Sulfidogenic microbial communities from technogenically transformed soils. Microbiology and Biotechnology, 2016; 2: 16-29. (In Russian) | |
| |
2. Antypchuk A.F., Pilyashenko-Novohatnyy, Yevdokymenko T.M. Practical Microbiology: manual for students. Kyiv: University "Ukraina", 2011. 155 p. (In Ukrainian) | |
| |
3. Arias Y.M., Tebo B.M. Cr(VI) reduction by sulfidogenic and nonsulfidogenic microbial consortia. Applied and Environmental Microbiology, 2003; 69(3): 1847-1853. | |
| |
4. Arinushkina E.V. Guidelines for the chemical analysis of soils. 2nd ed. Moscow: MSU, 1970. 488 p. (In Russian) | |
| |
5. Brown N.L., Lee B.T.O., Silver S. Bacterial transport and resistance to copper. Metal Ions in Biological Systems [ed. by Sigel H., Sigel A.]. New York: Marcel Dekker, 1994; 30: 405-435. | |
| |
6. Childers S.E., Ciufo S., Lovley D.R. Geobacter metallireducens accesses insoluble Fe(III) oxide by chemotaxis. Nature, 2002; 416: 767-769. | |
| |
7. Copper resistance B. Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ). UniProt [internet-resource]. - Access regime: http://www.uniprot.org/uniprot/B3E7K7. | |
| |
8. Diakiv S.V., Hnatush S.O., Moroz O.M. et. al. Sulfur reducing bacteria from coal pits waste heaps of Chervonograd mining region. Studia Biologica, 2016; 10(2): 63-76. | |
| |
9. DSTU 4726:2007. Soil quality. Determination of total nitrogen in modification of NSC ISSA named after O. N. Sokolovsky. Kyiv: Derzhspozhyvstandart of Ukraine, 2007. 19 p. (In Ukrainian) | |
| |
10. DSTU ISO11465-2001. Soil quality. Determination of dry matter and moisture content by mass. Gravimetric method. Kyiv: Derzhspozhyvstandart of Ukraine, 2002. 68 p. (In Ukrainian) | |
| |
11. GOST 26426-85. Soils. Method of sulfate ions in an aqueous extract determination. Moscow: Publishing House of Standards, 1985. P. 43-46. (In Russian) | |
| |
12. Gudz S.P., Нnatush S.O., Javorska G.V. et al. Practical Microbiology: manual for students. Lviv: LNU named after I. Franko, 2014. 436 p. (In Ukrainian) | |
| |
13. Halafiyan A. A. Statistica 6. Statistical data analysis. 3rd ed. Manual. Moscow: LLC "Binom-Press", 2007. 512 p. (in Russian) | |
| |
14. Han R., Li F., Liu T. et al. Effects of incubation conditions on Cr(VI) reduction by c-type cytochromes in Intact Shewanella oneidensis MR-1 cells. Frontiers in Microbiology, 2016; 7: 12 p. | |
| |
15. Harris D.С. Quantitative Chemical Analysis. 6th ed., 2003. 928 p. | |
| |
16. Karpinets L., Lobachevska O., Baranov V. et al. Total content of nitrogen and heavy metals in the mosses gametophyte and in upper layer of technogenic substrates of the mine dumps. Studia Biologica, 2017; 11(1): 101-108. (In Ukrainian) | |
| |
17. Lakyn G.F. Biometrics. Moscow: Vyshha Shkola, 1990. 352 p. (In Russian) | |
| |
18. Liesack W., Finster K. Phylogenetic analysis of five strains of gram-negative, obligately anaerobic, sulfur-reducing bacteria and description of Desulfuromusa gen. nov., including Desulfuromusa kysingii sp. nov., Desulfuromusa bakii sp. nov., and Desulfuromusa succinoxidans sp. nov. International Journal of Systematic Bacteriology, 1994; 44(4): 753-758. | |
| |
19. Lovley D.R. Dissimilatory metal reduction. Annual Review of Microbiology, 1993; 47: 263-290. | |
| |
20. Lovley, D.R., Ueki T., Zhang T. et al. Geobacter: The Microbe Electric's Physiology, Ecology, and Practical Applications. Advances in Microbial Physiology, 2011; 59: 1-100. | |
| |
21. Mahadevan R., Bond D.R., Butler J.E et. al. Characterization of metabolism in the Fe(III)-reducing organism Geobacter sulfurreducens by constraint-based modeling. Applied and Environmental Microbiology, 2006; 72(2): 1558-1568. | |
| |
22. Maslovska O., Hnatush S. The influence of ferric (III) citrate on ATP-hydrolases of Desulfuromonas acetoxidans ІМV В-7384. Visnyk of Dnipropetrovsk University. Biology, ecology, 2013; 21(1): 3-8. (In Ukrainian) | |
| |
23. Methodical recommendations for conducting field and laboratory studies of soils and plants by the control of environmental pollution by metals. Institute of Experimental Meteorology, Moscow State University named after M.V. Lomonosov [ed. by N.G. Zyrin, S.G. Malahov]. Moscow: Gidrometeoizdat, 1981. Р. 9-33. (In Russian) | |
| |
24. Moroz O. M. Hydrogen sulfide production by sulfur reducing bacteria under influence of hard metals. Visnyk of Lviv University. Biological series, 2013; 61:154-165. (In Ukrainian) | |
| |
25. Pastorella G. Investigation of the electrochemical activity of chromium tolerant mutants of Geobacter metallireducens: PhD thesis. Dublin City University, 2014 [internet-resource]. Access regime: http://doras.dcu.ie/19743/. | |
| |
26. Richter K., Schicklberger M., Gescher J. Dissimilatory reduction of extracellular electron acceptors in anaerobic respiration. Applied and Environmental Microbiology, 2012; 78(4): 913-921. | |
| |
27. Sugiyama M. Reagent composition for measuring hydrogen sulfide and method for measuring hydrogen sulfide. United States Patent N 6340596, 2002. | |
| |
28. Tashyrev O.B., Galinker E.V., Andreyuk K.I. Thermodynamic calculations of redox interaction of microorganisms with oxidative metals (Hg2+, CrO2−4 and Cu2+). Reports of the National Academy of Sciences of Ukraine, 2008; 4: 166-172. (In Ukrainian) | |
| |
29. Tashyrev O. Integral mechanisms of microbial groups and natural ecosystems interaction with toxic metals in the light of V. І. Vernadsky teaching. Svitogliad, 2013; 1: 66-73. (In Ukrainian) | |
| |
30. Vandieken V., Mussmann M. Niemann H. et al. Desulfuromonas svalbardensis sp. nov. and Desulfuromusa ferrireducens sp. nov., psychrophilic, Fe(III)-reducing bacteria isolated from Arctic sediments, Svalbard. International Journal of Systematic and Evolutionary Microbiology, 2006; 56: 1133-1139. | |
| |
31. Viti C., Marchi E., Decorosi F. et al. Molecular mechanisms of Cr (VI) resistance in bacteria and fungi. FEMS Microbiology Reviews, 2014; 38(4): 633-659. | |
| |
32. White C., Sayer J.A., Gadd G.M. Microbial solubilization and immobilization of toxic metals: key biogeochemical processes for treatment of contamination. FEMS Microbiology Reviews, 2000; 33: 197-208. |
Refbacks
- There are currently no refbacks.
Copyright (c) 2017 Studia biologica
This work is licensed under a Creative Commons Attribution 4.0 International License.