DYNAMICS OF CHLOROPHYLL AND PARAMYLON ACCUMULATION IN EUGLENA GRACILIS CELLS AT MIXOTROPHIC CULTIVATION

V. M. Mokrosnop


DOI: http://dx.doi.org/10.30970/sbi.1002.483

Abstract


Microalgae E. gracilis is capable of using ethanol as a carbon and energy source for growth both in the light and in the dark. Ethanol is efficiently utilized under illumination of the culture. At mixotrophic cultivation, the dynamics of accumulation of chlorophyll and paramylon – the main reserve polysaccharide of E. gracilis, was significantly different from the autotrophic control. Chlorophyll content in the mixotrophic cells at moderate intensities of light (100–250 µmol∙m-2 ·s-1) at the beginning of the exponential growth phase decreased and then increased steadily to reach a stationary growth phase. On the contrary, the content of paramylon, was maximal at the lag phase, and decreased in an exponential growth phase. Thus, the synthesis of photosynthetic pigment-containing complexes was delayed at the beginning of cultivation, in the presence of ethanol, and cell growth was mainly due to the substrate uptake. Probably, after assimilation of exogenous ethanol, the observed intensive growth of the culture was provided by the photosynthetic conversion of light energy and usage of carbon deposited in paramylon.

Keywords: Euglena gracilis, mixotrophic cultivation, ethanol, chlorophyll, paramylon.

 


Full Text:

PDF

References


1. Ahmadinejad N., Dagan T., Martin W. Genome history in the symbiotic hybrid Euglena gracilis. Gene, 2007; 402: 35-39.
https://doi.org/10.1016/j.gene.2007.07.023
PMid:17716833

2. Afiukwa C.A., Ogbonna J.C. Effects of mixed substrates on growth and vitamin production by Euglena gracilis. African Journal of Biotechnology, 2007; 6: 2612-2615.
https://doi.org/10.5897/AJB2007.000-2417

3. Andersen R.A. Algal Culturing Techniques. London: Elsevier Academic Press, 2005. 579.

4. App A.A., Jagendorf A.T. Repression of chloroplast development in Euglena gracilis by substrates. Eukaryotic Microbiology, 1963; 10: 340-343.
https://doi.org/10.1111/j.1550-7408.1963.tb01686.x

5. Barsanti L., Vismara R., Passarelli V., Gualtieri P. Paramylon (β-1,3-glucan) content in wild type and WZSI mutant of Euglena gracilis. Effects of growth contitions. Journal of Applied Phycology, 2001; 13: 59-65.
https://doi.org/10.1023/A:1008105416065

6. Baumer D., Preisfeld A., Ruppel H.G. Isolation and characterization of paramylon synthase from Euglena gracilis (EUGLENOPHYCEAE). Journal of Phycology, 2001; 37: 38-46.
https://doi.org/10.1046/j.1529-8817.2001.037001038.x

7. Beneragama C. K., Goto K. Chlorophyll a:b ratio increases under low-light in "shade-tolerant" Euglena gracilis. Tropical Agricultural Research, 2010; 22: 12-25.
https://doi.org/10.4038/tar.v22i1.2666

8. Coleman L.W., Rosen B.H., Schwartzbach S.D. Environmental control of carbohydrate and lipid in Euglena. Plant Cell Physiology, 1988; 29: 423-432.

9. Cook J.R. Influence of light on acetate utilization in green Euglena. Plant Cell Physiology, 1965; 6: 301-307.
https://doi.org/10.1093/oxfordjournals.pcp.a079101

10. Dubertret G. Functional and structural organization of chlorophyll in the developing photosynthetic membranes of Euglena gracilis Z. Plant Physiology, 1981; 67: 47-53.
https://doi.org/10.1104/pp.67.1.47
PMid:16661631 PMCid:PMC425619

11. DuBois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 1956; 28: 350-356.
https://doi.org/10.1021/ac60111a017

12. Freimund S., Sauter M., Kappeli O., Dutler H. A new non-degrading isolation process for 1,3-beta-D-glucan of high purity from baker's yeast Saccharomyces cerevisiae. Carbohydrate Polymers, 2003; 54: 159-171.
https://doi.org/10.1016/S0144-8617(03)00162-0

13. Garlaschi F. M., Garlaschi A. M., Lombardi A., Forti G. Effect of ethanol on the metabolism of Euglena gracilis. Plant Science Letters, 1974; 2: 29-39.
https://doi.org/10.1016/0304-4211(74)90035-2

14. Kiss J.Z., Vasconcelos C.A., Triemer R.E. The intramember particle profile of the paramylon membrane during paramylon synthesis in Euglena (EUGLENOPHYCEAE). Journal of Phycology, 1988; 24: 152-157.
https://doi.org/10.1111/j.1529-8817.1988.tb04229.x

15. Lichtenthaler H.K., Welburn A.R. Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents. Biochemical Society Transactions, 1983; 603: 591-593.
https://doi.org/10.1042/bst0110591

16. Marechal L.R., Goldemberg S.H. Uridine diphosphate glucose-beta-1,3-glucan beta-3-glucosyltransferase from Euglena gracilis. The Journal of Biological Chemistry. 1964; 239: 3163-3167.

17. Marzullo G., Danforth W. F. Composition of ethanol-insoluble assimilatory products of oxidative assimilation of acetate by Euglena graciis. Journal of General Microbiology, 1964; 34: 21-29.
https://doi.org/10.1099/00221287-34-1-21
PMid:14121217

18. Mokrosnop V.M., Zolotareva E.K. Influence of fungicides on the growth of the microalgal culture Euglena gracilis Klebs (Euglenophyta). International Journal on Algae, 2013; 15: 180-187.
https://doi.org/10.1615/InterJAlgae.v15.i2.60

19. Mokrosnop V.M., Zolotareva E.K. Microalgae as tocopherol producers. Biotechnologia Acta, 2014; 7: 26-33. (In Russian)
https://doi.org/10.15407/biotech7.02.026

20. Mokrosnop V.M., Polishchuk A.V., Zolotareva E.K. The functional state of the photosynthetic apparatus of Euglena gracilis cells at the mixotrophic cultivation. Reports of the National Academy of Sciences of Ukraine, 2015; 10: 77-84. (In Ukrainian)
https://doi.org/10.15407/dopovidi2015.10.077

21. Mokrosnop V.M., Polishchuk A.V., Zolotareva E.K. Accumulation of α-tocopherol and β-carotene in Euglena gracilis cells under autotrophic and mixotrophic culture conditions. Applied Biochemistry and Microbiology, 2016; 52: 216-221.
https://doi.org/10.1134/S0003683816020101

22. Monfils A.K., Triemer R.E., Bellairs E.F. Characterization of paramylon morphological diversity in photosynthetic euglenoids (Euglenales, Euglenophyta). Phycologia, 2011; 50:156-169.
https://doi.org/10.2216/09-112.1

23. Mykhaylenko N.F., Syvash O.O., Tupik N.D., Zolotareva O.K. Exogenous hexoses cause quantitative changes of pigment and glycerolipid composition in filamentous cyanobacteria. Photosynthetica, 2004; 42: 105-110.
https://doi.org/10.1023/B:PHOT.0000040577.30424.d1

24. Rikin A., Schwartzbach S.D. Regulation by light and ethanol of the synthesis of the light-harvesting chlorophyll a/b-binding protein of photosystem II in Euglena. Planta, 1989; 178: 76-83.
https://doi.org/10.1007/BF00392529
PMid:24212552

25. Rodriguez-Zavala J.S., Ortiz-Cruz M.A., Mendoza-Hernanderz G., Moreno-Sanchez R. Increased synthesis of α-tocopherol, paramylon and tyrosine by Euglena gracilis under conditions of high biomass production. Journal of Applied Microbiology, 2010; 109: 2160-2172.
https://doi.org/10.1111/j.1365-2672.2010.04848.x
PMid:20854454

26. Stepanov S.S., Zolotareva E.K. Photosynthesis, respiration and growth rate of Сhlamydomonas reinhardtii on exogenic ethanol application. Microbiology and Biotechnology, 2014; 3: 63-71. (In Ukrainian)
https://doi.org/10.18524/2307-4663.2013.4(24).48977

27. Yamane Y., Utsunomiya T., Watanabe M., Sasaki K. Biomass production in mixotrophic culture of Euglena gracilis under acidic condition and its growth energetic. Biotechnology Letters, 2001; 23: 1223-1228.
https://doi.org/10.1023/A:1010573218863

28. Zolotariova O.K., Shniukova E.I., Sivash O.O. et al. Prospects of microalgae using in bіo­technology. Kyiv: Alterpres, 2008. 234. (In Ukrainian)

29. Zolotaryova O., Shnyukova E. Where biofuel industry goes to? Visn. of NAS of Ukraine, 2010; 4: 10-20. (In Ukrainian)


Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Studia biologica