THE CONTENT OF FREE AMINO ACIDS IN THE RED CLOVER PLANTS UNDER THE INFLUENCE OF OIL POLLUTED SOIL
DOI: http://dx.doi.org/10.30970/sbi.1002.473
Abstract
The influence of conditions of oil polluted soil on the general content of free amino acids and proline in the red clover plants (Trifolium pratense L., sort Peredkarpatska 6) at early stages of ontogenesis was studied. It was found that the content of free amino acids increased in organs of red clover plants under the influence of oil pollution of soil. The content of free amino acids under the influence of oil polluted soil increased more substantialy in the plants’ leaves. The amount of proline in the red clover leaves increased 7 times under induction by soil pollution with oil. The maximum increase in free amine acids content under the effect of oil pollution of soil was at the primary stage of red clover plants growth – the stage of cotyledon leaves. At the upcoming of following phenological phase – the first real and first trifoliate leaf – the induction by oil pollution of soil increase in the amount of both proline and general content of free amino acids in organs of red clover plants was less expressed. Obtained results suggest that free amino acids and proline play a significant protective role at primary phases of red clover plants’ growth in the soil polluted with oil.
Keywords
Full Text:
PDF (Українська)References
1. Atanasova E. Effect of nitrogen sources on the nitrogenous forms and accumulation of amino acid in head cabbage. Plant, Soil and Environment, 2008; 54: 66-71. | |
| |
2. Diaz P., Betti M., Sanchez D.H. et al. Deficiency in plastidic glutamine synthetase alters proline metabolism and transcriptomic response in Lotus japonicus under drought stress, New Phytol, 2010; 188: 1001-1013. | |
| |
3. Dovgajuk-Semenuk M.V., Velychko O.I., Terek O.I. Тhe content of ammonium and nitrate nitrogen in the red clover plants under the influence of oil polluted soil and fertalization with phosphorus-potassium fertilizers. Scientific Notes ТNPU, 2015; 1(62): 94-99. (In Ukrainian) | |
| |
4. Hayat S., Hayat Q., Alyemeni M. et al. Role of proline under changing environments. Plant Signal Behav, 2012; 7(11): 1456-1466. | |
| |
5. Ivanchenko О. E. Influence of heavy metals on nitrogen metabolism of plants. Problems of Bioindications and Ecology, 2009; sites.znu.edu.ua. (In Ukrainian) | |
| |
6. Kamenova-Yuchimenko St., Georgieva V., Markovska J. Changes in the quantity of aminо acids and soluble proteins as regardes pea plants in an answer to Cd toxicity in the rpesenhce of some physiologically - active substance. Bulg. J. Plant Physiol, Spec. Issul, 2003: 397. | |
| |
7. Kavi Kishor P. B., Sreenivasulu N. Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue? Plant Cell Environ, 2014; 37: 300-311. | |
| |
8. Kobyletska M.S., Terek O.I. Effect of salicylic acid on free amino acids content in wheat Triticum aestivum L. and sunflower Helianthus annuus L. under the actions of cadmium ions. Studia Biologica, 2012; 6(1): 87-92. (In Ukrainian) | |
| |
9. Kolupaev Yu.E., Vayner A.A., Yastreb T.O. Рroline: physiological functions and regulation of its content in plants under stress conditions. The Bulletin of Kharkiv National Agrarian University, 2014; 2 (32): 6-22. (In Ukrainian) | |
| |
10. Kolupaev Yu.Ye., Karpets Yu.V. Рarticipation of soluble carbohydrates and low-molecular nitrogen compounds in adaptive reactions of plants. The Bulletin of Kharkiv National Agrarian University, 2010; 2 (20): 36-53. (In Ukrainian) | |
| |
11. Kosakivska I.V., Golovyanko I.V. Аdaptation of plants: biosinthesis and functions of stress proteins. Ukrainian Phytosociological Collection, 2006; 24: 3-17. (In Ukrainian) | |
| |
12. Li Z., Peng Y., Zhang X. et al. Exogenous spermidine improves water stress tolerance of white clover (Trifolium repens L.) involved in antioxidant defence, gene expression and proline metabolism. Plant. Omics. Journal, 2014; 7(6): 517-526. | |
| |
13. Liang X., Zhang L., Natarajan S.K. et al. Proline mechanisms of stress survival. Antioxidants & Redox Signaling, 2013; 19(9): 998-1011. | |
| |
14. Malenka U., Kobyletska М., Terek О. Іnfluence of salicylic acid on the amount of free amino acids and proline in plants of wheat and corn under drought conditions. Studia Biologica, 2014; 8(2): 123-132. (In Ukrainian) | |
| |
15. Neuberg M., Pavlíková D., Pavíнk M. et al. The effect of different nitrogen nutrition on proline and asparagine content in plant. Plant Soil Environ, 2010; 56(7): 305-311. | |
| |
16. Pochinok H.N. Methods of Analysis Biochemically Plants. Kiev: Science Thought, 1976. P. 72-95. (In Russian) | |
| |
17. Sadasivam S., Manickam А. Biochemical methods. New Age International, New Delhi, 1996; 56-140. | |
| |
18. Terek O., Lapshyna O., Velychko O. et al. Crude oil contamination and plants. Journal of Central European Green Innovation, 2015; 3(2): 175-184. | |
| |
19. Terek O.I., Patsula O.І. Plant growth and development. Lviv: Ivan Franko LNU, 2011: P. 259-262. (In Ukrainian) | |
| |
20. Vayner A.O., Kolupaev Yu.E., Yastreb T.O. et al. Еxogenous proline suppresses the increase in the activities of antioxidant enzymes of wheat seedlings caused by heat hardening. The Bulletin of Kharkiv National Agrarian University, 2014; 1(31): 66-71. (In Ukrainian) | |
| |
21. Velychko O.І. The Influence of the oil polluted soil conditions on the Red clover protein spectral composition. Scientific Journal NLTUU, 2014; 24(9): 115-117. (In Ukrainian) |
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Studia biologica
This work is licensed under a Creative Commons Attribution 4.0 International License.