STRUCTURAL AND FUNCTIONAL CHANGES IN THE CELLS OF MICROORGANISMS UNDER THE INFLUENCE OF HYDROGEN SULFIDE

A. A. Halushka, S. P. Gudz


DOI: http://dx.doi.org/10.30970/sbi.0302.039

Abstract


Influence of hydrogen sulfide on the survival of Pichia guilliermondii yeasts and Bacillus subtilis і Desulfovibrio desulfuricans bacteria cells was investigated. The most sensitive to the influence of hydrogen sulfide were Ddesulfuricans, and the most resistant – Pguilliermondii. Hydrogen sulfide caused changes in cell ultrastructure. In Bsubtilis and Pguilliermondi, the plasmolysis, membrane structures and cell wall destruction and accumulation of metals sulfides in the cytoplasm were observed.


Keywords


hydrogen sulfide, toxicity, survival, ultrastructure

References


1. Бреслер Е.Г., Переумов Д.А., Глазунов Е.А. Исследование оперона биосинтеза рибофлавина у Bacillus subtilis. Определение содержания АТФ: рибофлавин-5′-фосфотрансферазы в клетках с розличным фенотипом. Генетика, 1997; 13: 880-887.

2. Галушка А.А., Перетятко Т.Б., Гудзь С.П. Вплив сірководню на Saccharomyces cerevisiae. Мікробіологія і біотехнологія, 2008; 3: 49-57.
https://doi.org/10.18524/2307-4663.2008.3(4).105035

3. Крешков А.И. Основы аналитической химии. Теоретические основы. Качественный анализ. Книга первая. Изд. 4-е, перераб. М.: Химия, 1976. 472 с.

4. Лакин Г.Ф. Биометрия. М.: Высш. шк., 1990. 352 с.

5. Перетятко Т., Гнатуш С., Гудзь С. Сульфатвідновлювальні бактерії Яворівського сіркового родовища. Мікроб. журнал, 2006; 68 (5): 84-91.

6. Перетятко Т.Б., Гнатуш С.О., Гудзь С.П. Утворення сульфіду Desulfovibrio desulfuricans Ya-11 за різних умов культивування. Вісник Львів. ун-ту. Серія біол, 2007; 43: 180-184.

7. Burkcholder P. Influence of some environmental factors upon the production of riboflavin by yeasts. Arch. Biochem, 1943; 1 (1): 121-130.

8. Cohen Y., Jorgensen B.B., Revsbech N.P., Poplawski R. Adaptation to hydrogen sulfide of oxygenic and anoxygenic photosynthesis among cyanobacteria. Appl. Env. Microbiol,1986; 51 (2): 398-407.

9. Dean G.A. A simple colorymetric finish for the Johnson-Nishita micro-distillation of sulfur. Analist, 1966; 91 (1085): 530-532.
https://doi.org/10.1039/an9669100530

10. Espie G.S., Miller A.G., Canvin D.T. Selective and reversible inhibition of active CO2 transport by hydrogen sulfide in a cyanobacterium. Plant Physiol, 1989; 91: 387-394.
https://doi.org/10.1104/pp.91.1.387
PMid:16667030 PMCid:PMC1062004

11. Hydrogen sulfide: human health aspects [Electronic resource]. World health organization: Cicads 53. Geneva, 2003. Access mode:
http://www.inchem.org/documents/cicads/cicads/cicad53.htm

12. Lloyd K.G., Edgcomb V.P., Molyneaux S.J. et al. Effects of dissolved sulfide, pH, and temperature on growth and survival of marine hyperthermophilic Archaea. Appl. Env. Microbiol, 2005; 71(10): 6383-6387.
https://doi.org/10.1128/AEM.71.10.6383-6387.2005
PMid:16204562 PMCid:PMC1265996

13. Miller S.R., Bebout B.M. Variation in sulfide tolerance of photosystem II in phylogenetically diverse cyanobacteria from sulfidic habitats. Appl. Env. Microbiol, 2004; 70 (2): 736-744.
https://doi.org/10.1128/AEM.70.2.736-744.2004
PMid:14766549 PMCid:PMC348820

14. Postgate J. R. The sulfate-reducing bacteria, 2nd ed. Cambridge: Cambridge University Press, 1984. 208 p.

15. Reynolds E.S. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell. Biol, 1963; 17: 208-212.
https://doi.org/10.1083/jcb.17.1.208
PMid:13986422 PMCid:PMC2106263

16. Пат. 6340596 США, МКИ G 01 N 33/00. Reagent composition for measuring hydrogen sulfide and method for measuring hydrogen. Masami Sugiyama (Японія); Fujirebio Inc. N248316; Заявл. 02.11.1999; Опубл. 22.01.2002; НКИ 436/121. 9 с.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2009 Studia biologica

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.