APPLICATION OF MULTI-MARKER APPROACH FOR ASSESSMENT OF STRESS SYNDROME IN TRANSPLANTED MUSSELS  DREISSENA POLYMORPHA

H. I. Falfushynska; L. L. Gnatyshyna; O. B. Stoliar; O. Dedourge-Geffard; A. Geffard

doi:10.30970/sbi.0401.066

APPLICATION OF MULTI-MARKER APPROACH FOR ASSESSMENT OF STRESS SYNDROME IN TRANSPLANTED MUSSELS DREISSENA POLYMORPHA

H. I. Falfushynska, L. L. Gnatyshyna, O. B. Stoliar, O. Dedourge-Geffard, A. Geffard

DOI: http://dx.doi.org/10.30970/sbi.0401.066

Abstract

Zebra mussels Dreissena polymorpha were transferred for 14, 28, 60 and 120 days from their reference site (C) to sites with agricultural pollution (A), industrial pollution from the alcohol industry (I1), or manufactured wastes (I2). The aim for that was to determine their ability to reflect various types of stressful conditions. General temporal patterns of mussels were confirmed by Centroid grouping and Discriminant Function analysis of the battery of their biochemical markers. After 14 days of transplantation, mussels demonstrated most prominent inter-site differences. In site A, a decreasing of the activity of oxidative defense enzymes took place, demonstrating weak stress response. In both sites I, increased levels of lipid peroxidation, metallothioneins and ethoxyresorufin-O-deethylase indicated oxidative injury, pollution by heavy metals, and persistent organic compounds, respectively. Low cholinesterase activity was detected in both C and B sites, showing the pollution by agricultural discharges. Prolonged exposure to polluted environment provoked the endocrine disruption (high levels of vitellogenin-like proteins), oxidative stress and decrease of glutathione and metallothionein concentrations, especially in the industrial sites.

Keywords

Dreissena polymorpha, oxidative stress, biomarkers of exposure, pollution, field transplantation

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References

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