A COMPARISON OF PACLITAXEL AND VINBLASTINE INDUCED GIANT CELL FORMATION IN MURINE NK/Ly LYMPHOMA
DOI: http://dx.doi.org/10.30970/sbi.0501.129
Abstract
We compared giant cell formation in murine NK/Ly lymphoma under treatment with anticancer drugs – vinblastine or paclitaxel. It was found that vinblastine caused an appearance of bigger cells than paclitaxel did, whereas paclitaxel induced more rapid formation of giant cells than vinblastine did. Differences were also revealed in morphology of lymphoma cells treated with these anticancer drugs. While vinblastine induced more intensive membrane blebbing comparing with paclitaxel action, paclitaxel induced more pronounced cytosol vacuolization. Evaluation of trans-membrane potential based on studying mitochondrial accumulation of fluorescent dye Rhodamine-123, showed that paclitaxel caused more significant decrease of this potential comparing with vinblastine action. Role of the revealed peculiarities in the action of vinblastine and paclitaxel towards tumor cells are considered.
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1. Gascoine K.E., Taylor S.S. How do anti-mitotic drugs kill cancer cells? Journal of Cell Science, 2009; 122(15): 2579-2585. | |
| |
2. Jordan M.A., Wilson L. Microtubules as a target for anticancer drugs. Nature Reviews. Cancer, 2004; 4(4): 253-265. | |
| |
3. Wang L.G., Liu X.M., Kreis W., Budman D.R. The effect of antimicrotubule agents on signal transduction pathways of apoptosis: a review. Cancer Chemotherapy and Pharmacology, 1999; 44(5): 355-361. | |
| |
4. Tolmach L.J., Marcus P.L. Development of X-ray induced giant HeLa cells. Experimental Cell Research, 1960; 20: 350-360. | |
| |
5. Brito D. A., Yang Z., and Rieder C. L. Microtubules do not promote mitotic slippage when the spindle assembly checkpoint cannot be satisfied. J. Cell Biol, 2008; 182: 623-629. | |
| |
6. McGrogana B.T., Gilmartina B., Carneyc D.N., McCann A. Taxanes, microtubules and chemoresistant breast cancer. Biochimica et Biophysica Acta, 2008; 1785(2): 96-132. | |
| |
7. Kavalaris M., Sasha Tait A., Walsh B.J. et al. Multiple microtubule alterations are associated with Vinca alkaloids resistance in human leukemia cells. Cancer Research, 2001; 61: 5803-5809. | |
| |
8. Mitchison T.J., Kirschner M. Dynamic instability of microtubule growth. Nature (Lond.), 1984; 312: 237-242. | |
| |
9. Jordan M.A., Wilson L. Microtubules and actin filaments: dynamic targets for cancer therapy. Curr. Opin. Cell Biol, 1998; 10: 123-130. | |
| |
10. Lootsik M.D., Boiko N.M., Lootsik M.M. Investigation of therapeutic effect of saponin from greater celandine on a model of murine lymphoma NK/Ly. 2nd Ukrainian congress for cell biology (Book of Аbstracts), 2007; 1: 103. (In Ukrainian) | |
| |
11. Horbay R.O., Manko B.O., Manko V.V. et al. Respiration characteristics of mitochondria in parental and giant transformed cells of murine Nemeth-Kellner lymphoma, Cell Biol. Int, 2011: a submitted article. | |
| |
12. Lutsyk M.M., Yashchenko A.M. Quantitive method of evaluation of tumor cells damage under the influence of antitumor drugs in vivo by cytochemical staining for protein. Reports of Morphology, 2009; 15(1): 188-192. | |
| |
13. Erenpreisa J.A., Cragg M.S. Mitotic death: a mechanism of survival? A review. Cancer Cell Int, 2001; 1:1. | |
| |
14 . Vakifahmetoglu H., Olsson M., Zhivotovsky B. Death through a tragedy: mitotic catastrophe, Cell Death and Differentiation, 2008; 15: 1153-1162. | |
| |
15. Kaminskyy V., Kulachkovskyy O., Stoika R. A decisive role of mitochondria in defining rate and intensity of apoptosis induction by different alkaloids. Toxicology Letters, 2008; 177: 168-181. | |
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