PATTERNS OF EXPRESSION OF LIPID-TRANSFER PROTEIN GENE IN ORGANS OF SCOTS PINE (PINUS SYLVESTRIS L.)

N. I. Hrunyk, V. A. Kovaleva, R. T. Gout


DOI: http://dx.doi.org/10.30970/sbi.0602.210

Abstract


The patterns of lipid-transfer protein PsLTP1 (Pinus sylvestris lipid transfer protein 1) gene expression in the vegetative and generative organs of Scots pine have been studied by the reverse transcription polymerase chain reaction (RT-PCR). It was found that PsLTP1 gene expression is tissue-specific and it changes during ontogenetic development. Effect of phytohormones on PsLTP1 expression level in Scots pine seedlings has been investigated.


Keywords


Scots pine, lipid-transfer protein PsLTP1, expression

References


1. Груник Н.І., Ковальова В.А., Гут Р.Т. Органоспецифічна експресія ліпідтрансферного протеїну на різних етапах онтогенетичного розвитку сосни звичайної. В кн.: Регуляція росту і розвитку рослин: фізіолого-біохімічні та генетичні аспекти: матер. ІІ Міжнар. конф. (м. Харків, 11-13 жовтня 2011 р.). Харків, 2011. с. 21-22.

2. Груник Н.І., Шаловило Ю.І., Ковальова В.А., Гут Р.Т. Ліпідтрансферні протеїни рослин: структура, функції і біотехнологічні перспективи. Наук. вісник НЛТУ України: збірник наук.-техн. праць, 2011; 21(8): 32-35.

3. Гут Р.Т., Юсипович Ю.М., Ковальова В.А. Особливості експресії генів дефензинів у різних органах сосни звичайної (Pinus sylvestris L.). Наук. праці Лісівничої академії наук України: збірник наук. праць, 2011; 9: 86-89.

4. Boutrot F., Guirao A., Alary R. et al. Wheat nonspecific lipid transfer protein genes display a complex pattern of expression in developing seeds. Biochim. Biophys. Acta, 2005; 1730: 114-125.
https://doi.org/10.1016/j.bbaexp.2005.06.010
PMid:16061294

5. Castro M.S., Fontes W. Plant defense and antimicrobial peptides. Protein and Peptide Letters, 2005; 12(1): 11-16.
https://doi.org/10.2174/0929866053405832

6. Chang S., Puryear J., Cairney J. A simple and efficient method for isolating RNA from pine trees. Plant Mol. Biol. Rep, 1993; 11: 113-116.
https://doi.org/10.1007/BF02670468

7. Douliez J.P., Michon T., Elmorijani K. et al. Structure, biological and technological functions of lipid transfer proteins and indolines, the major lipid binding proteins from cereal kernels. J. Cereal Sci, 2000; 32: 1-20.
https://doi.org/10.1006/jcrs.2000.0315

8. Eklund D.M., Edqvist J. Localization of nonspecific lipid transfer proteins correlate with programmed cell death responses during endosperm degradation in Euphorbia lagascae seedlings. Plant Physiology, 2003; 132: 1249-1259.
https://doi.org/10.1104/pp.103.020875
PMid:12857807 PMCid:PMC167065

9. Fleming A.J., Mandel T., Hofmann S. et al. Expression pattern of a tobacco lipid transfer protein gene within the shoot apex. Plant J, 1992; 2: 855-862.
https://doi.org/10.1046/j.1365-313X.1992.t01-2-00999.x
PMid:1302637

10. Garcia-Olmedo F., Molina A., Segura A. et al. The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol, 1995; 3: 72-74.
https://doi.org/10.1016/S0966-842X(00)88879-4

11. Gastaminza G., Lombardero M., Bernaola G. et al. Allergenicity and cross-reactivity of pine pollen. Clin. Exp. Allergy, 2009; 39(9): 1438-46.
https://doi.org/10.1111/j.1365-2222.2009.03308.x
PMid:19573163

12. Jung H.W., Kim W., Hwang B.K. Three pathogen-inducible genes encoding lipid transfer protein from pepper are differentially activated by pathogens, abiotic, and environmental stresses. Plant, Cell and Environment, 2003; 26: 915-928.
https://doi.org/10.1046/j.1365-3040.2003.01024.x

13. Kader J.C. Lipid-transfer proteins in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol, 1996; 47: 627-654.
https://doi.org/10.1146/annurev.arplant.47.1.627
PMid:15012303

14. Kader J.C. Lipid-transfer proteins: a puzzling family of plant proteins. Trends Plant Sci,1997; 2: 66-70.
https://doi.org/10.1016/S1360-1385(97)82565-4

15. Kinlaw C.S., Gerttula S.M., Carter M.C. Lipid transfer protein genes of loblolly pine are members of a complex gene family. Plant Mol. Biol, 1994; 26: 1213-1216.
https://doi.org/10.1007/BF00040702
PMid:7811979

16. Kovaleva V., Kiyamova R., Cramer R. et al. Purification and molecular cloning of antimicrobial peptides from Scots pine seedlings. Peptides, 2009; 30(12): 2136-2143.
https://doi.org/10.1016/j.peptides.2009.08.007
PMid:19683554

17. Molina A., Segura A., Garcia-Olmedo F. Lipid transfer proteins (LTPs) from barley and maize leaves are potent inhibitirs of bacterial and fungal plant pathogens. FEBS Letter, 1993; 316. 627-654.
https://doi.org/10.1016/0014-5793(93)81198-9

18. Moor R., Clark W.D., Stern K.R. Botany. Wm. C. Brown Publishers, 1995. 824 p.

19. Ostergaard J., Hojrup P., Knudsen J. Amino acid sequences of three acyl-binding/lipid-transfer proteins from rape seedlings. Biochim. Biophys. Acta, 1995; 1254: 169-179.
https://doi.org/10.1016/0005-2760(94)00180-7

20. Park S.Y., Lauh G.Y., Mollet J.C. et al. A lipid transfer-like protein is necessary for lily pollen tube adhesion to an in vitro stylar matrix. Plant Cell, 2000; 12: 151-164.
https://doi.org/10.2307/3871036
PMid:10634914 PMCid:PMC140221

21. Sabala I., Elfstrand M., Farbos I. et al. Tissue-specific expression of Pa18, a putative lipid transfer protein gene, during embryo development in Norway spruce (Picea abies). Plant Mol. Biol, 2000; 42: 461-478.
https://doi.org/10.1023/A:1006303702086
PMid:10798616

22. Salzman R.A., Brady J.A., Finlayson S.A. et al. Transcriptional Profiling of Sorghum Induced by Methyl Jasmonate, Salicylic Acid, and Aminocyclopropane Carboxylic Acid Reveals Cooperative Regulation and Novel Gene Responses. Plant Physiology, 2005; 138: 352-368.
https://doi.org/10.1104/pp.104.058206
PMid:15863699 PMCid:PMC1104189

23. Sanchez-Monge R., Lombardero M., Garcia-Selles F.J. et al. Lipid-transfer proteins are relevant allergens in fruit allergy. J. Allergy Clin. Immunol, 1999; 103: 514-519.
https://doi.org/10.1016/S0091-6749(99)70479-3

24. Sawano Y., Hatano K., Miyakawa T. et al. Proteinase inhibitor from ginkgo seeds is a member of the plant nonspecific lipid transfer protein gene family. Plant Physiology, 2008; 146: 1909-1919.
https://doi.org/10.1104/pp.107.111500
PMid:18305212 PMCid:PMC2287358

25. Sohal A.K., Pallas J.A., Jenkins G.I. The promoter of a Brassica napus lipid transfer protein gene is active in a range of tissues and stimulated by light and viral infection in transgenic Arabidopsis. Plant Mol. Biol, 1999; 41: 75-87.
https://doi.org/10.1023/A:1006232700835
PMid:10561070

26. Sossountzov L., Ruiz-Avila L., Vignols F. et al. Spatial and temporal expression of a maize lipid transfer protein gene. The Plant Cell, 1991; 3: 923-933.
https://doi.org/10.1105/tpc.3.9.923
PMid:1822992 PMCid:PMC160060

27. Trevino M.B., O'Connell M.A. Three drought-responsive members of the nonspecific lipid-transfer protein gene family in Lycopersicon pennellii show different developmental patterns of expression. Plant Physiology, 1998; 116: 1461-1468.
https://doi.org/10.1104/pp.116.4.1461
PMid:9536064 PMCid:PMC35054

28. Vignols F., Wigger M., Garcia-Garrido J.M. et al. Rice lipid transfer protein (LTP) genes belong to a complex multigene family and are differentially regulated, Gene, 1997; 195: 177-186.
https://doi.org/10.1016/S0378-1119(97)00137-6

29. Warren J.M., Covert S.F. Differential expression of pine and Cronartium quercuum f. sp. fusiforme genes in fusiform rust galls. Appl. Environ. Microbiol, 2004; 70: 441-451.
https://doi.org/10.1128/AEM.70.1.441-451.2004
PMid:14711673 PMCid:PMC321230

30. Yubero-Serrano E., Moyano E., Medina-Escobar N. Identification of a strawberry gene encoding a non-specific lipid transfer protein that responds to ABA, wounding and cold stress. J. of Exp. Botany, 2003; 54(389): 1865-1877.
https://doi.org/10.1093/jxb/erg211
PMid:12869521


Refbacks

  • There are currently no refbacks.


Copyright (c) 2012 Studia biologica

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.