IDENTIFICATION AND PROPERTIES OF THE PURPLE PHOTOSYNTHETIC SULFUR BACTERIA OF GENUS THIORHODOCOCCUS ISOLATED FROM SAKI LAKE (AR CRIMEA)
DOI: http://dx.doi.org/10.30970/sbi.0602.231
Abstract
The phototrophic bacteria were isolated from water and silt of Saki lake (AR Crimea). The were brown-red coloured. This bacteria used hydrogen sulfide in anaerobic photosyntetical process and stored elemental sulfur inside the cells. According to physiological, biochemical, morphological properties, this bacteria was ascribed to Chromatiaceaе family. The 16S DNA gene sequence analysis based on using Blastn program revealed that investigated consecution has 94% similarity to Thiohalocapsa halophila, Thiorhodococcus bheemlicus, Marinimicrobium koreense, Marichromatium bheemlicum, and 93% similarity to Thiorhodococcus minor, Thiorhodovibrio winogradsky of Gammaproteobacteria domein. Based on the study of morphological, physiological characteristics and 16S rRNA nucleotide gene sequences, phototrophic purple bacteria of Sа-2010/А strain from water of Saki lake (AR Crimea) belong to Thiorhodococcus genus.
Keywords
Full Text:
PDF (Українська)References
1. Кондратьева Е.Н. Фотосинтезирующие бактерии. Москва: Изд-во Москов. ун-та, 1989. 346 с. | |
| |
2. Ксензенко В.И. Технология брома и иода. Москва: Наука, 1960. 304 с. | |
| |
3. Кушкевич І.В., Гнатуш С.О., Гудзь С.П. Зміна деяких фізіолого-біохімічних властивостей Thiocapsa roseopersicina за впливу різних концентрацій кадмій сульфату. Вісник Одеськ. нац. ун-ту, 2008; 13(4): 177-189. | |
| |
4. Мусієнко М.М., Паршикова Т.В., Славний П.С. Спектрофотометричні методи в практиці фізіології, біохімії та екології рослин. Київ: Фітосоціоцентр, 2001. 200 с. | |
| |
5. Павлова Ю.О., Гудзь С.П. Бактерії родини Chromatiaceae, виділені з озера «Яворівське» Язівського сіркового родовища. Вісник Харк. нац. ун-ту. Сер. біол, 2008; 7(14): 148-154. | |
| |
6. Родина А. Г. Методы водной микробиологии. Практ. руководство. М.;Л.: Наука, 1965. 363 с. | |
| |
7. Хоулт Д., Криг Н., Снит П. и др. Определитель бактерий Берджи. Москва: Мир, 1997. 540 с. | |
| |
8. Anil Kumar P. Sasi Jyothsna, Srinivas T. et al. Two novel species of marine phototrophic Gammaproteobacteria: Thiorhodococcus bheemlicus sp. nov. and Thiorhodococcus kakinadensis sp. nov. JSEM, 2007, 57(11): 2458-2461. | |
| |
9. Barns S.M, Pelletier D.A, Lane D.J. Weisburg W.G. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol, 1991, 173(2): 697-703. | |
| |
10. Britton G. General carotenoid methods. Meth. Enzymol, 1985; 3(В): 113-145. | |
| |
11. Bryantseva I.A., Gorlenko V.M., Kompantseva E.I. Thiorhodospira sibirica gen.nov., sp. nov., a new alkaliphilic purple sulfur bacterium from a Siberian soda lake. J. Syst. Bacteriol, 1999; 49: 69 -703. | |
| |
12. Gemerden H. Growth measurements of Chromatium cultures. Arhiv. Mikrobiol, 1968; 64: 103-110. | |
| |
13. Gorlenko V.M., Bryantseva I.A, Rabold S. et al. Ectothiorhodospira variabilis sp. nov., an alkaliphilic and halophilic purple sulfur bacterium from soda lakes. JSEM, 2009, 59(4): 658-64. | |
| |
14. Imhoff J. Caumette P. Recommended standards for description of new species of anoxygenic phototrophic bacteria. J. Syst. Bacteriol, 2004; 54: 1415-1421. | |
| |
15. Imhoff J., Suling J., Petri R. Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. JSEM, 1998, 48 (4): 1129-1143. | |
| |
16. Koizumi Y., Kojima K., Oguri K. Vertical and temporal shifts of microbial communities in the water column and sediment of saline meromictic Lake Kaiike (Japan), as determinated by a 16S rDNA-based analysis, and related to physicochemical gradients. Appl. Environ. Microbiol, 2004; 6: 622-637. | |
| |
17. Peduzzi S., Welsh A., Demarta A. et al. Thiocystis chemoclinalis sp. nov. and Thiocystis cadagnonensis sp. nov., motile purple sulfur bacteria isolated from the chemocline of a meromictic lake. JSEM, 2011, 61(7):1682-1687. | |
| |
18. Puchkova N. N., Imhoff J. F., Gorlenko V. M. Thiocapsa litoralis sp. nov., a new purple sulfur bacterium from microbial mats from the White Sea. JSEM, 2000, 50 (4): 1441-1447. | |
| |
19. Rabold S., Gorlenko V., Imhoff J. Thiorhodococcus mannitoliphagus sp. nov., a purple sulfur bacterium from the White Sea. JSEM, 2006, 56(8): 1945-1951. | |
| |
20. Reynolds E.S. The use of lead citrate at pH as an electronopaque stain in electron microscopy. J. Cell Biol, 1963; 17: 208-212. | |
| |
21. Sorensen K., Canfield D., Oren A. Salinity responses of benthic microbial communities in a solar saltern (Eilat, Israel). Appl. Environ. Microbiol, 2004; 70(3): 1608-1616. | |
| |
22. Zaar A., Fuchs G., Golecki JR., Overmann J. A new purple sulfur bacterium isolated from a littoral microbial mat, Thiorhodococcus drewsii sp. nov. Arch Microbiol, 2003, 179(3): 174-183. | |
| |
23. ГОСТ 26426-85. Почвы. Метод определения ионов сульфата в водной вытяжке. Москва: Изд-во стандартов, 1985. | |
| |
24. Пат. 6340596 США, МКИ G 01 N33/00. Reagent composition for measuring hydrogen sulfide and method for measuring hydrogen / Masami Sugiyama (Японія); Fujirebio Inc. № 248316; Заявл. 02.01. 1999; Опубл. 22.01.2002; НКИ 436.121. 9 с. |
Refbacks
- There are currently no refbacks.
Copyright (c) 2012 Studia biologica
This work is licensed under a Creative Commons Attribution 4.0 International License.