THE INFLUENCE OF THE WATER-SALT EXTRACT OF THE MEDICINAL LEECH HIRUDO VERBANA CARENA, 1820 ON THE GENERAL COURSE OF EMBRYOGENESIS IN RATS AFTER INTRAPERITONEAL ADMINISTRATION

Ruslan Aminov


DOI: http://dx.doi.org/10.30970/sbi.1702.713

Abstract


Background. The increase in infectious and non-infectious diseases occurrence during pregnancy prompts scientists to search for substances that can fight these ailments. This especially applies to natural substances that have virtually no side effects. These include substances obtained from medicinal leeches. These substances have a wide range of therapeutic effects; as to their practical use, all preclinical studies must be completed. This particularly refers to the presence of an embryotoxic effect. The aim of this work was to investigate the effect of the water-salt extract of the medicinal leech Hirudo verbana Carena, 1820 on the general course of embryogenesis in rats when administered intraperitoneally.
Materials and Methods. The work was performed on female non-linear white rats. Two experimental groups of animals were injected intraperitoneally with a water-salt extract of the medicinal leech at a concentration of 5 μg/g of the animal, in a volume of 0.5 mL, three times before mating and three times during pregnancy every 3 days. Two control groups were injected intraperitoneally with physiological saline according to the same scheme as the experimental groups. Subsequently, one of the control and experimental groups underwent euthanasia, dissection of the abdominal cavity and uterine horns on the 20th day of pregnancy. We analyzed the total number of corpora lutea of pregnancy, the number of live and dead fetuses. Fetuses were weighed, examined for the presence of skin hematomas, mutilations, and external abnormalities in the develop­ment of internal organs. The other two groups of control and experimental animals were left until the offspring were born. Then the females of both groups were weighed, and their general condition was assessed. Newborn rats were examined, weight and body length were determined, mortality was recorded from the beginning of birth to 15 days, the presence of skin hematomas, mutilations, and external anomalies of the development of internal organs were checked.
Results and discussion. The study established that the intraperitoneal injection of a water-salt extract of a medicinal leech before and during pregnancy in female rats does not cause any toxic effect during embryogenesis and at the early stages of post-embryonic ontogenesis. During the external examination of fetuses and offspring in both groups, no developmental anomalies were noted, all organs in both groups were of normal topography and size. The number of subcutaneous hematomas in the experimental group of fetuses compared to the control group decreased by an average of 50%, postnatal mortality decreased by an average of more than 3 times, as well as an increase in body weight in females, an increase in body weight and body length in the fetus and offspring in the early post-embryonic period ontogenesis (p <0.05).
Conclusion. Intraperitoneal administration of a therapeutic dose of a water-salt extract of the medicinal leech to female rats before and during pregnancy, after every three days, does not have any toxic effect on the course of embryogenesis and later in early post-embryonic ontogenesis.


Keywords


medicinal leeches, embryotoxicity, teratogenicity, rats

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References


Abdisa, T. (2018). Therapeutic importance of leech and impact of leech in domestic animals. MOJ Drug Design Development & Therapy, 2(6), 235-242.
Google Scholar

Afroz, A. T., Najar, F. A., Faisal, M., & Fatima, M. (2020). Effect of Irsale Alaq (leech therapy) in thrombosed pile mass: a case study. International Journal of Scientific Reports, 6(6), 220-222. doi:10.18203/issn.2454-2156.intjscirep20202098
CrossrefGoogle Scholar

Akalın, Ç., & Ekmen, N. (2020). Non-occlusive mesenteric ischemia due to hirudotherapy: a case report. Cureus, 12(7), e9467. doi:10.7759/cureus.9467
CrossrefPubMedPMCGoogle Scholar

Amani, L., Motamed, N., Mirabzadeh Ardakani, M., Dehghan Shasaltaneh, M., Malek, M., Shamsa, F., Fatemi, E., & Amin, M. (2021). Semi-solid product of medicinal leech enhances wound healing in rats. Jundishapur Journal of Natural Pharmaceutical Products, 16(4), e113910. doi:10.5812/jjnpp.113910
CrossrefGoogle Scholar

Aminov, R. F. (2022). Pryrodnyy imunomodulyator iz til medychnykh p'yavok: otrymannya ta zastosuvannya [Natural immunomodulator from the bodies of medical leeches: production and application]. Zaporizhzhia: Zaporizhia National University. Retrieved from https://files.znu.edu.ua/files/Bibliobooks/Inshi67/0049507.pdf (In Ukrainian)

Aminov, R., Aminova, A., & Makyeyeva, L. (2022). Morphological parameters of spleen and thymus of the male rats on the basis of the hirudological influence of Hirudo verbena. Annals of Parasitology, 68(1), 55-60. doi:10.17420/ap6801.408
PubMedGoogle Scholar

Aminov, R. & Aminova, A. (2021). Indirect effect of substances of the hemophagous parasite Hirudo verbana on the immune system of the host rats. Annals of Parasitology, 67(4), 603-610. doi:10.17420/ap6704.376
PubMedGoogle Scholar

Aminov, R. F., Frolov, O. K., Fedotov, E. R., & Lytvynenko, R. O. (2018). Sposib otrymannya imunomodulyatora [Method of obtaining an immunomodulator]. Ukrainian patent (No UA 125736). Ministry for Development of Economy and Trade. Retrieved from https://base.uipv.org/searchINV/search.php?action=viewdetails&IdClaim=247561&chapter=description (In Ukrainian)

Canpolat, İ., & Sağlam, N. (2004). Treatment of aural hematomas in dogs with the medicinal leech, Hirudo medicinalis. Doğu Anadolu Bölgesi Araştırmaları, 2(2) 67-69.
Google Scholar

Curcio, J., & Lloyd, C. M. (2020). Leech me alone! Atraumatic hemarthrosis after hirudotherapy. Cureus, 12(2), e6915. doi:10.7759/cureus.6915
CrossrefPubMedPMCGoogle Scholar

Davoodi, F., Taheri, S., Raisi, A., Rajabzadeh, A., Zakian, A., Hablolvarid, M. H., & Ahmadvand, H. (2021). Leech therapy (Hirudo medicinalis) attenuates testicular damages induced by testicular ischemia/reperfusion in an animal model. BMC Veterinary Research, 17(1), 1-15. doi:10.1186/s12917-021-02951-5
CrossrefPubMedPMCGoogle Scholar

Fattakhov, N. K., Tilyakhodzhaeva, G. B., & Abdulkhakimov A. R. (2021). Efficiency of application of hirudotherapy to have been having coronaviral infection. Journal of Applied Research, 7(4), 2908-2911. doi:10.47191/rajar/v7i4.03
CrossrefGoogle Scholar

Frolov, A. Kopeyka, V., Fedotov, E., Kapustin, S., & Lytvynenko, R. (2010). Vliyaniye girudoterapii na fiziologicheskiye pokazateli u koz [The effect of hirudotherapy on physiological parameters in goats]. Tvarynnytstvo Ukrainy, 7, 7-10. (In Russian)
Google Scholar

Grytsaj, I. R., Mandzynets S. M., & Bura, M. V. (2022). Influence of the sodium fluoride on the development and survival of the loach embryos. Biophysical Bulletin, 47: 13-26. doi:10.26565/2075-3810-2022-47-02 (In Ukrainian)
Google Scholar

Huang, H., Lei, R., Li, Y., Huang, Q., Gao, N., & Zou, W. (2021). Hirudo (Leech) for proliferative vitreous retinopathy: a protocol for systemic review and meta-analysis. Medicine, 100(3), e24412. doi:10.1097/md.0000000000024412
CrossrefPubMedPMCGoogle Scholar

Nefodova, O. O. & Halperin, O. I. (2018). Comparison of embryotropic effect of cadium salts on embryogenesis of rats in experiment. Bulletin of Problems Biology and Medicine, 3(145), 305-309. doi:10.29254/2077-4214-2018-3-145-305-309 (In Ukrainian)
CrossrefGoogle Scholar

Nowicki, A., Jaworska, J., & Baranski, W. (2021). Leech therapy in the treatment of a penile haematoma in a stallion. Veterinární Medicína, 66(6), 266-271. doi:10.17221/163/2020-vetmed
CrossrefScholar

Ojo, P. O., Babayi, H., Olayemi, I. K., Peter, O. O., Fadipe, L. A., Baba, E., & Izebe, K. (2018). Anti-tubercular activities and molecular characterization of salivary extract of leech (Hirudo medicinalis) against Mycobacterium tuberculosis. Journal of Tuberculosis Research, 6(1), 1-9. doi:10.4236/jtr.2018.61001
CrossrefGoogle Scholar

Rehman, S. (2020). Management of diabetic foot ulcer by Hirudo medicinalis, the "healing leech". Diabetic Foot Ulcer, 315-330. doi:10.1007/978-981-15-7639-3_19
CrossrefGoogle Scholar

Shamelashvili, K. L., & Shatorna, V. F. (2021). Embryotoxic effect of cadmium chloride on rat body. Bulletin of Problems Biology and Medicine, 1(159), 147-150. doi:10.29254/2077-4214-2021-1-159-147-150 (In Ukrainian)
CrossrefGoogle Scholar

Shatorna, V. F., & Krasnov, O. O. (2022). Chronic influence of cadmium chloride on rat embryogenesis in isolated and combined with zinc and copper sucсinates intragastric administration in the experiment. Ukrainian Journal of Medicine, Biology and Sport, 7(2), 254-260. doi:10.26693/jmbs07.02.254 (In Ukrainian)
CrossrefGoogle Scholar

Shatorna, V. F., & Rudenko, K. M. (2020). Determination of the degree of embryotoxicity of cadmium chloride by enteral administration throughout pregnancy in rats. Bulletin of Problems Biology and Medicine, 3(157), 66-70. doi:10.29254/2077-4214-2020-3-157-66-70 (In Ukrainian)
CrossrefGoogle Scholar

Sobczak, N., & Kantyka, M. (2014). Hirudotherapy in veterinary medicine. Annals of Parasitology, 60(2), 89-92.
PubMedGoogle Scholar

Sudhadevi, M. (2020). Leech therapy: a holistic treatment. International Journal of Advance Research in Nursing, 3(1), 130-132.
Google Scholar

Trenholme, H. N., Masseau, I., & Reinero, C. R. (2021). Hirudotherapy (medicinal leeches) for treatment of upper airway obstruction in a dog. Journal of Veterinary Emergency and Critical Care, 31(5), 661-667. doi:10.1111/vec.13094
CrossrefPubMedGoogle Scholar

Yang, F., Li, Y., Guo, S., Pan, Y., Yan, C., & Chen, Z. (2021). Hirudo lyophilized powder ameliorates renal injury in diabetic rats by suppressing oxidative stress and inflammation. Evidence-Based Complementary and Alternative Medicine, 2021, 1-12. doi:10.1155/2021/6657673
CrossrefPubMedPMCGoogle Scholar


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