I. V. Kushkevych



The sulfate-reducing bacteria have been isolated from human intestine. These bacteria are identified by their morphological, physiological and biochemical characteristics as the Desulfovibrio sp. and Desulfomicrobium sp. according to Bergey’s Manual of Determinative Bacteriology. Growth of the isolated strains of bacteria has been studied under various conditions including types of media, effect of oxygen, pH, and tempe­rature. Bacterial growth in the modified Kravtsov-Sorokin’s medium, and process of dissimilatory sulfate reduction by the selected strains of bacteria have been investiga­ted. All obtained bacterial cultures used sulfate and lactate intensively as well as produced hydrogen sulfide and acetate in the medium. The Desulfovibrio sp. strain Vib-7 produced the highest concentration (up to 3.23 mM) of hydrogen sulfide, while the bacteria used about 99% of sulfate ions presented in the medium. This strain also produced the highest concentration of acetate ions (up to 15.87 mM) on the fifth day of cultivation. Among the studied Desulfomicrobium sp. strains, the highest concentration of hydrogen sulfide was produced by the Desulfomicrobium sp. strain Rod-9, and the highest concentration of acetate ions was produced by the Desulfomicrobium sp. strain Rod-4.


sulfate-reducing bacteria, Desulfovibrio, Desulfomicrobium, identification, sulfates, hydrogen sulfide

Full Text:



1. Bailey N.T.J. Statistical Methods in Biology. Third edition. Cambridge University Press, 1995. 255 p.

2. Barton L.L., Hamilton W.A. Sulphate-reducing Bacteria. Environmental and Engineered. Cambridge University Press, 2007. 553 p.

3. Beerens H., Romond C. Sulfate-reducing anaerobic bacteria in human feces. The American Journ. of Clin. Nutr, 1977; 30: 1770-1776.

4. Brenner D.J., Krieg N.R., Staley J.T., Garrity G.M. Bergey's manual of Systematic Bacteriology. Vol. Two: The Proteobacteria, Part C: The Alpha-, Beta-, Delta-, and Epsilonproteobacteria. Second Edition. Printed in the United States of America, 2005. 1388 p.

5. Campbell M.K., Farrell S.O. Biochemistry. Sixth Edition. Publisher: Cengage Learning, 2008. 800 p.

6. Cummings J.H., Macfarlane G.T., Macfarlane S. Intestinal Bacteria and Ulcerative Colitis. Curr. Issues Intest. Microbiol, 2003; 4: 9-20.

7. Furutani A., Schindler D.W. Effects of lake acidification on rates of organic matter decomposition. Limnol. Oceanogr, 1984; 29: 687-694.

8. Gibson G.R., Cummings J.H., Macfarlane G.T. Growth and activities of sulphate-reducing bacteria in gut contents of health subjects and patients with ulcerative colitis. FEMS Microbiol. Ecol, 1991; 86: 103-112.

9. Gibson G.R., Macfarlane G.T., Cummings J.H. Sulphate-reducing bacteria and hydrogen metabolism in the human large intestine. Gut, 1993; 34: 437-439.
PMid:8491386 PMCid:PMC1374298

10. Gibson G.R., Macfarlane S., Macfarlane G.T. Metabolic interactions involving sulfate-reducing and methanogenic bacteria in the human large intestine. FEMS Microbiol. Ecol, 1993; 12: 117-125.

11. Holt J.G., Krieg N.R., Sneath P.H. et al. Bergey's Manual of Determinative Bacteriology. Ninth Edition. Moscow: Mir, 1997; 1. 426 p.

12. Isakova O.P., Tarasevych Yu.Yu., Yuzjuk Yu.I. Processing and visualization of data with the physical experiments by usage Origin package. Moscow: Book House "LIBKOM", 2009; 136 p.

13. Karavayko G.I., Kuznetsov S.I., Golomzik A.I. A role of microorganisms in a leaching of metals from ores. Moscow: Science, 1972. 248 p.

14. Kolmert A., Wikstrom P., Hallberg K.B. A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures. Journ. of Microbiol. Methods, 2000; 41: 179-184.

15. Kushkevych I.V. Sulfate-reducing bacteria of the human intestine. I. Dissimilatory sulfate reduction. Sci Int Jour Biological Studies/Studia Biologica, 2012; 6(1): 149-180.

16. Kushkevych I.V. Sulfate-reducing bacteria of the human intestine. II. The role in the diseases development. Sci Int Jour Biological Studies/Studia Biologica, 2012; 6(2): 221-250.

17. Kushkevych I.V., Moroz O.M. Growth of various strains of sulfate-reducing bacteria of human large intestine. Sci Int Jour Biological Studies/Studia Biologica, 2012; 6(3): 115-124.

18. Langendijk P.S., Kulik E.M., Sandmeier H. et al. Isolation of Desulfomicrobium orale sp. nov. and Desulfovibrio strain NY682, oral sulfate-reducing bacteria involved in human periodontal disease. J. Syst. Evol. Microbiol, 2001; 51(3): 1035-1044.

19. Loubinoux J., Bronowicji J.P., Pereira I.A. et al. Sulphate-reducing bacteria in human feces and their association with inflammatory diseases. FEMS Microbiol. Ecol, 2002; 40: 107-112.

20. Loubinoux J., Mory F., Pereira I.A., Le Faou A.E. Bacteremia caused by a strain of Desulfovibrio related to the provisionally named Desulfovibrio fairfieldensis. J. Clin. Microbiol, 2000; 38: 931-934.

21. Macfarlane S., Dillon J.F. Microbial biofilms in the human gastrointestinal tract. J. Appl. Microbiol, 2007; 102: 1187-1196.

22. Pitcher M.C., Cummings J.H. Hydrogen sulphide: a bacterial toxin in ulcerative colitis? Gut, 1996; 39: 1-4.
PMid:8881797 PMCid:PMC1383219

23. Postgate J.R. The sulfate-reducing bacteria. Second edition. Cambridge Univ. Press, 1984. 199 p.

24. Rowan F.E., Docherty N.G., Coffey J.C., O'Connell P.R. Sulphate-reducing bacteria and hydrogen sulphide in the aetiology of ulcerative colitis. British Journal of Surgery, 2009; 96: 151-158.

25. Sugiyama M. Reagent composition for measuring hydrogen sulfide and method for measuring hydrogen / U.S. Pat. 6340596 B1 USA, Int. Cl. G 01 N 33/00, 2002.

26. Sutton S. Measurement of Microbial Cells by Optical Density. Journal of Validation Technology, 2011; 17(1): 46-49.

27. Vlizlo V.V., Fedoruk R.S., Makar I.A. et al. Physiological and biochemical methods of researches in biology, stockbreeding and veterinary medicine. Handbook: Institute of Animal Biology. Third Edition: revised and enlarged. Lviv, 2004. 402 p.

28. Zinkevich V.V., Beech I.B. Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa. FEMS Microbiol. Ecol, 2000; 34: 147-155.


  • There are currently no refbacks.

Copyright (c) 2013 Studia biologica

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.