Y. T. Salyha



Chlorpyrifos is one of the most common pesticides around the world. This organophosphorus compound inhibits of acetylcholinesterase activity. A toxicity of chlorpyrifos is not limited to inhibition of cholinesterase activity, but may act through other mechanisms, including breaking antioxidant-prooxidant balance. The goal of the study was to conduct a comparative analysis of key indicators of the antioxidant defense system in different brain regions of rats intoxicated with chlorpyrifos. Wistar male rats were injected intraperitoneally once with chlorpyrifos in 15 and 30 mg/kg. After 24 hours, the animals were decapitated under ether anesthesia, after which the brain was removed immediately, and hippocampus, cerebellum and cerebral cortex were isolated. In selected areas of the brain activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione content were determined. It was found that intoxication of rats by chlorpyrifos caused a decreasing of key enzymatic parameters of antioxidant system in brain tissue. Different parts of brain characterised by different antiradical defense activity. The highest intensity of antioxidant processes was observed in the cerebral hemispheres of rat brain, while the lowest – in the cerebellum.


chlorpyrifos, brain, superoxide dismutase, catalase, glutathione pero­xi­dase, glutathione reductase, glutathione reduced


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