O. Dolaychuk, R. Fedoruk



Studied of macro- and microstructure of kidney, liver and spleen of female and male rats did not show significant deviations from such structures of the control group. It has been found that structure of spleen follicles of female and male control groups of rat is better expressed comparing to those whose diet included soybean. The animals whose diet included both conventional and transgenic soybean were found to have a slight increase of Bowman’s capsule in kidney tissues enabled by glomerulus reduction that is apparently due to the effect of both soybean varieties of biologically active components on water-salt metabolism in animals. Considering that the internal structure was preserved and conformed with the norms for animals of given species and age in both control and experimental female and male rat groups, a conclusion on the absence of significant negative or positive effect of GM soybean components on the histological structure of liver, spleen and kidneys can be proposed.


genetically modified soybean, histology of internals, liver, spleen, kidney

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1. Flachowsky G., Chesson A., Aulrich K. Animal nutrition with feeds from genetically modified plants. Arch. Anim. Nutr, 2005; 59(1): 1-40.

2. European Food Safety Authority (EFSA) GMO Panel Working Group on Animal Feeding Trials. Safety and nutritional assessment of GM plants and derived food and feed: The role of animal feeding trials. Food Chemistry and Toxicology, 2008; 46: 2-70.

3. Патрушев М. В. Возняк М. В. Генетически модифицированные источники: характеристика некоторых ГМ-линий, их детекция. Партнеры и конкуренты, 2004; 10: 19-26.

4. Taylor N.B., Kishore G.M. Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Sci, 1995; 35: 1451-1461.

5. Astwood J.D., Leach J.N., Fuchs R.L. Stability of food allergens to digestion in vitro. Nature Biotechn, 1996; 14: 1269-1273.

6. Nelson K.A. Renner K.A. Weed management in wide- and narrow-row glyphosate-resistant soybean. J. Prod. Agric, 1999; 12: 460-465.

7. Aumaitrea A., Aulrichb K., Chesson A. et al. New feeds fromgenetically modified plants: Substantial equivalence, nutritional equivalence, digestibility and safety for animals and the food chain. Lives.t Prod. Sci, 2002; 74: 223-228.

8. Aumaitre A., Aulrich K., Chesson A. et al. Soybean meal from roundup ready or conventional soybeans in diets for growing-finishing swine. J. Anim. Sci, 2002; 80: 708-715.

9. Cromwell G. L., Lindemman M. D., Randolph, J.H. et al. Soybean meal from roundup ready or conventional soybeans in diets for growing-finishing swine. J. Anim. Sci, 2002; 80(3):708-15.

10. Faust M.A. New feeds from genetically modified plants: The US approach to safety for animals and the food chain. Livest. Prod. Sci, 2002; 74: 239-254.

11. Schubbert R., Renz D., Schmitz B. et al. Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA. Proc. Natl. Acad. Sci. USA, 1997; 94: 961-966.
PMid:9023365 PMCid:PMC19622

12. Tony M. A., Butschke A., Broll H. et al. Safety assessment of BT 176maize in broiler nutrition: Degradation of maize-DNA and its metabolic fate. Arch. Anim. Nutr, 2003; 57: 235-252.

13. Spiroux J., Roullier F., Cellier D. et al. A comparison of the effects of three GM corn varieties on mammalian health. Int. J. Biol. Sci, 2009; 5:706-726.
PMid:20011136 PMCid:PMC2793308

14. Séralini G. E., Cellier D., Spiroux J. New analysis of a rat feeding study with a genetically modified maize reveals signs of hepatorenal toxicity. Arch. Environ. Contam. Toxicol, 2007; 52: 596-602.

15. Kilic A., Akay M. T. A three generation study with genetically modified Bt corn in rats: biochemical and histopathological investigation. Food Chem. Toxicol, 2008; 46: 1164-1170.

16. Параняк Р.П., Вудмаска І.В., Параняк М.Р. та ін. Оцінка компоцизійної еквівалентності генетично модифікованої (GTS 40-3-2) та немодифікованої сої за амінокислотним та жирнокислотним складом. Здобутки клін. і експеримент. медицини, 2009, 1(10): 117-120.

17. European convention for the protection of vertebrate animals used for experiments and other scientific purposes. Strasbourg: Coun. of Europe, 1986. 53 p.

18. Волкова О.В. Елецкий Ю.К. Основы гистологии с гистологической техникой. М.: Медицина, 1982. 303 с.

19. Malatesta M., Caporaloni C., Gavaudan S. et al. Ultrastructural morphometrical and immunocytochemical analyses of hepatocyte nuclei from mice fed on genetically modified soybean. Cell Structure and Function, 2002; 27:173-180.

20. Malatesta M., Tiberi C., Baldelli B. et al. Reversibility of hepatocyte nuclear modifications in mice fed on genetically modified soybean. European Journal of Histochemistry, 2005; 49: 237-242.

21. Sakamoto Y., Tada Y., Fukumori N. et al. A 52-week feeding study of genetically modified soybeans in F344 rats. J. Food Hyg. Soc. Japan, 2007; 48: 41-50.

22. Sakamoto Y., Tada Y., Fukumori N. et al. A 104-week feeding study of genetically modified soybeans in F344 rats. J. Food Hyg. Soc. Japan, 2008; 49: 272-282.

23. Malatesta M., Boraldi F., Annovi G. et al. A long-term study on female mice fed on a genetically modified soybean: effects on liver ageing. Histochem. Cell Biol, 2008; 130: 967-977.

24. Appenzeller L.M., Munley S.M., Hoban D. et al. Subchronic feeding study of herbicide-tolerant soybean DP-356Ø43-5 in Sprague Dawley rats. Food Chem. Toxicol, 2008; 46: 2201-2213.

25. Delaney В., Appenzeller L.M., Munley S.M. et al. Subchronic feeding studv of high oleic acid soybeans (event DP-305423-1) in Sprague-Dawley rats. Food Chem. Toxicol, 2008; 46: 3808-3817.

26. Teshima R., Akiyama H., Okunuki H. et al. Effect of GM and non-GM soybeans on the immune svstem of BN rats and B10A mice. J. Food Hyg. Soc. Japan, 2000; 41: 188-193.

27. Trabalza-Marinucci M., Brandi G., Rondini C. et al. A three-year longitudinal study on the effects of a diet containing genetically modified Bt176 maize on the health status and performance of sheep. Livestock Sci, 2008; 113: 178-190.

28. Baranowski A., Rosochacki S., Parada R. et al. The effect of diet containing genetically modified triticale on growth and transgenic DNA fate in selected tissues of mice. Anim. Sci. Pap. Rep, 2006; 24: 129-142.

29. Tudisco R., Mastellone V., Cutrignelli M.I. et al. Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings. Animal, 2010; 1: 1-10.

30. Cirnatu D., Jompan A., Sin A.I. et al. Multiple organ histopathological changes in broiler chickens fed on genetically modified organism. Rom. J. Morphol. Embryol, 2011; 52(1): 475-480.

31. Zhu Y., Li D., Wang F. et al. Nutritional assessment and fate of DNA of soybean meal from Roundup Ready or conventional soybeans using rats. Arch. Anim. Nutr, 2004; 58: 295-310.


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