PARAMETERS OF ANTIOXIDATIVE SYSTEM OF DESULFUROMONAS ACETOXIDANS UBDER THE INFLUENCE OF IRON (III) CITRATE AND SILVER NITRATE
DOI: http://dx.doi.org/10.30970/sbi.0701.253
Abstract
Desulfuromonas acetoxidans are obligative anaerobic sulfur bacteria of the aquatic sedimental environments that are able to Fe3+-dissimilative reduction. The influence of various concentrations of ferric (III) citrate and silver nitrate on specific catalase activity and reduced glutathione synthesis by D. аcetoxidans cells has been determined under their cultivation with fumarate addition and with absence of sulfur. Specific catalase activity increased with enhancing of ferric (III) citrate concentration and duration of bacterial cultivation under the addition of this salt. Under the influence of ferric (III) citrate, maximal catalase activity has been observed on the fourth day of cultivation with addition of 5 mM of investigated metal salt. Under the influence of ferric (III) citrate, increasing in reduced glutathione content has been observed on the second day of bacterial growth with addition of 1 mM of investigated metal salt. The highest specific catalase activity was determined on the second day of bacterial growth under the influence of all concentration range of investigated metal salt. The reduced glutathione content under silver nitrate exposure varied depending on the cultivation time and metal concentration. The maximum reduced glutathione content has been observed on the second day of bacterial growth under the effect of 6 µM of AgNO3.
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1. Василів О., Гнатуш С. Вплив сполук феруму та мангану на вміст глутатіону у клітинах сірковідновлювальних бактерій Desulfuromonas acetoxidans. Біологічні студії / Studia Biologica, 2011; 5(1): 5–10.
https://doi.org/10.30970/sbi.0501.133
2. Лакин Г.Ф. Биометрия. М.: Высшая школа, 1990. 352 с.
3. Меньшикова Е.Б., Ланкин В.З., Зенков Н.К. Окислительный стресс. Прооксиданты и антиоксиданты. М.: Слово, 2006. 556 с.
4. Чайка О., Перетятко Т. Сірковідновлювальні бактерії водойм Язівського сіркового родовища. Наук. вісн. Ужгород. ун-ту. Сер. Біол, 2010; 28: 52–55.
5. Barton L., Hamilton W. Sulfate-Reducing Bacteria. Environmental and Engineered Systems. New York, USA: Cambridge University Press, 2007. 558. | |
| |
6. Derek L. Dissimilatory Fe(III) and Mn(IV) Reduction. Adv. Мicrobial. Рhysiol, 2004; 49: 246-259. | |
| |
7. Derek L. Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals. Arh. Microbiol, 1993; 159: 336-344 | |
| |
8. Fareleira P., Santos B. Response of a strict anaerobe to oxygen: survival strategies in Desulfovibrio gigas. Microbiology, 2003; 149: 1513-1522. | |
| |
9. Papanikolaou G, Pantopoulos K. Iron metabolism and toxicity. Toxicology and Applied Pharmacology, 2005; 202: 199-211. | |
| |
10. Paulsen J., Kroger A., Thauer R. ATP-driven succinate oxidation in the catabolism of the Desulfuromonas acetoxidans. Arh. Microbiol, 1986; 144: 78-83. | |
| |
11. Vasyliv O., Hnatush S. Effect of transition metal compounds on catalase activity of sulfur-reducing bacterial Desulfuromonas acetoxidans cells. Visnyk of the Lviv University. Series Biology, 2011; 57: 207-215. | |
| |
12. Weisener C. Microbial dissolution of silver jаrosite: examining its tarce metal behaviour in reduced environments. J. Geomicrobiol, 2008; 25: 415-424. |
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