Biol. Stud. 2015: 9(1); 97–108 • DOI: https://doi.org/10.30970/sbi.0901.393

MICROWAVE RADIATION INFLUECE ON FREE-RADICAL PROCESSES AND Nа+, K+-ATPase ACTIVITY IN LOACH EMDRYONS

M. M. Yaremchuk, M. V. Dyka, D. I. Sanagursky


DOI: http://dx.doi.org/10.30970/sbi.0901.393

Abstract


This article is devoted to the investigation of experimental results of radiofrequency electromagnetic radiation effect on lipid peroxidation processes, Na+, K+-АТPase acti­vity and antioxidant protective system enzyme activity in loach embryos. It was established that microwave radiation leads to intensification of lipid peroxidation processes and decrease of Na+, K+-pump activity during early embryogenesis. Under the effect of electromagnetic radiation, we have admitted an increase in superoxide dismutase activity; whereas the glutathione peroxidase activity decreased at each developmental stage. Catalase activity decreased at the I, IV, VI, VIII stages of blastomere division, however, it had opposite effect on the Х stage of division. Using two-factor analysis of variance, the estimation of microwave radiation effect (1; 5; 10; 20 min exposure) and developmental time (І, IV, VI, VIII, Х stages of blastomere division) on investigated rates during early embryogenesis has been provided. It has been shown that microwave radiation plays a significant role on free-radical process variability, Na+, K+-АТPase activity, the activity of superoxide dismutase and glutathione peroxidase and is one of the strongest factor (р≥0.999).

Keywords: microwave radiation, loach embryos, lipid peroxidation, Na+, K+-ATPase, enzymes of the antioxidant defense system.

References


1. Aitken R.J., Bennetts L.E., Sawyer D. et al. Impact of radiofrequency electromagnetic radiation on DNA integrity in the male germline. International Journal of Andrology, 2005; 28(3): 171-179.
https://doi.org/10.1111/j.1365-2605.2005.00531.x
PMid:15910543

2. Andreoli Sh., McTeer J., Seifert S., Kempson S. Oxidant-induced alterations in glucose and phosphate transport in LLC-PK1 cells: Mechanism of injury. American Journal of Physiology, 1993; 265: 337-384.
https://doi.org/10.1152/ajprenal.1993.265.3.F377
PMid:8214096

3. Bodera P., Stankiewicz W., Zawada K. Changes in antioxidant capacity of blood due to mutual action of electromagnetic field (1800 MHz) and opioid drug (tramadol) in animal model of persistent inflammatory state. Pharmacological Reports, 2013; 65: 421-428.
https://doi.org/10.1016/S1734-1140(13)71017-X

4. Boldyrev A.A., Rouge E.K., Smirnova I.N., Tabak M. Na, K-ATPase the role of lipids and Mg-ions in the activity regulation. FEBS Letters, 1977; 80(2): 303-307.
https://doi.org/10.1016/0014-5793(77)80463-8

5. Burlaka A., Tsybulin O., Sidorik E., Lukin S., Polishuk V., Tsehmistrenko S., Yakymenko I. Overproduction of free radical species in embryonal cells exposed to low intensity radiofrequency radiation. Exp Oncol, 2013; 35(3): 219-225.

6. Diem E., Schwarz C., Adlkofer F. et al. Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. Mutation Research, 2005; 583(2): 178-183.
https://doi.org/10.1016/j.mrgentox.2005.03.006
PMid:15869902

7. Fiske C.H., Subbarow Y. The Сolorimetric Determination of Phosphorus. Journal of Biological Chemistry, 1925; 66: 375-400.

8. Grigor'ev Iu.G. Biological effects of mobile phone electromagnetic field on chick embryo. Radiats. Biol. Radioecol, 2003; 43(5): 541-543. (In Russian)

9. Güler G., Tomruk A., Ozgur E. et al. The effect of radiofrequency radiation on DNA and lipid damage in female and male infant rabbits. International Journal of Radiation Biology, 2012; 88(4): 367-373.
https://doi.org/10.3109/09553002.2012.646349
PMid:22145622

10. Hardell L., Carlberg M., Söderqvist F., Mild K.H. Long-term use of cellular phones and brain tumours: increased risk associated with use for > or =10 years. Occupational and Environmental Medicine, 2007; 64 (9): 626-632.
https://doi.org/10.1136/oem.2006.029751
PMid:17409179 PMCid:PMC2092574

11. Korolyuk M.A., Ivanova L.I., Mayorova I.G. Method for determination of catalase activity. Laboratornoe Delo, 1988; 1: 16-19. (In Russian)

12. Kostiuk V.A., Potapovich A.I., Kovaleva Zh.V. A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation. Vopr. Med. Khim, 1990; 36(2): 88-91. (In Russian)

13. Kovacic P., Somanathan R. Electromagnetic fields: mechanism, cell signaling, other bioprocesses, toxicity, radicals, antioxidants and beneficial effects. Journal of Receptors and Signal Transduction, 2010; 30(4): 214-226.
https://doi.org/10.3109/10799893.2010.488650
PMid:20509751

14. Kukreja R.C., Weaver A.B., Hess M.L. Sarcolemmal Na, K-ATPase: Inactivation by neutrophil-derived free radicals and oxidants. American Journal of Physiology, 1990; 259: 1330-1336.
https://doi.org/10.1152/ajpheart.1990.259.5.H1330
PMid:2173423

15. Lai H., Singh N.P. Magnetic-field-induced DNA strand breaks in brain cells of the rat. Environmental Health Perspectives, 2004; 112(6): 687-694.
https://doi.org/10.1289/ehp.6355
PMid:15121512 PMCid:PMC1241963

16. Lowry O.H., Rosenbrough N.J., Farr A.L., Randall R. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 1951; 193(1): 265-275.

17. Menshchikova E.B. Oxidative stress. Prooxidants and antioxidants. Moscow: Word, 2006. 556 p. (In Russian)

18. Moin V.M. A simple and specific method for determining glutathione peroxidase activity in erythrocytes. Laboratornoe Delo, 1986; 12: 724-727. (In Russian)

19. Moussa S.A. Oxidative stress in rats exposed to microwave radiation. Romanian Journal of Biophysics, 2009; 19(2): 149-158.

20. Nazıroglu M., Yuksel M., Kose S.A., Ozkaya M.O. Recent reports of Wi-Fi and mobile phone-induced radiation on oxidative stress and reproductive signaling pathways in females and males. Journal of Membrane Biology, 2013; 246: 869-875.
https://doi.org/10.1007/s00232-013-9597-9
PMid:24105626

21. Neifach A.А. Molecular biology of developmental processes. Moscow: Nauka, 1977. 311 р. (In Russian)

22. Nylund R., Leszczynski D. Mobile phone radiation causes changes in gene and protein expression in human endothelial cell lines and the response seems to be genome- and proteome-dependent. Proteomics, 2006; 6(17): 4769-4780.
https://doi.org/10.1002/pmic.200600076
PMid:16878295

23. Oktem F., Ozguner F., Mollaoglu H. et al. Oxidative damage in the kidney induced by 900-MHz- emitted mobile phone: protection by melatonin. Archives of Medical Research, 2005, 36: 350-355.
https://doi.org/10.1016/j.arcmed.2005.03.021
PMid:15950073

24.3 Oleksiuk N.P., Yanovych V.G. The activity of pro-and antoxidant systems in the liver of freshwater fishes in different seasons. Ukrainian Biochemical Journal, 2010; 82(3): 41-48. (In Ukrainian)

25. Rao V.S., Titushkin I.A., Moros E.G. et al. Nonthermal effects of radiofrequency-field exposure on calcium dynamics in stem cell-derived neuronal cells: elucidation of calcium pathways. Journal of Radiation Research, 2008; 169(3): 319-329.
https://doi.org/10.1667/RR1118.1
PMid:18302487

26. Sanagursky D.I. Objects of Biophysics: Monograph. Lviv: Publishing Center of Ivan Franko National University of Lviv, 2008. 522 p. (In Ukrainian)

27. Shahin S., Singh V.P., Shukla R.K. et al. 2.45 GHz microwave irradiation-induced oxidative stress affects implantation or pregnancy in mice, Mus musculus. Applied Biochemistry and Biotechnology, 2013; 169(5): 1727-1751.
https://doi.org/10.1007/s12010-012-0079-9
PMid:23334843

28. Timirbulatov R.A., Seleznev E.I. Method for increasing the intensity of free radical oxidation of lipid-containing components of the blood and its diagnostic significance. Laboratornoe Delo, 1981, 4: 209-211. (In Russian)

29. Yakymenko I.L., Henshel D., Sidorik E.P. et al. Effect of mobile phone electromagnetic radiation on somitogenesis of birds. Reports of the National Academy of Sciences of Ukraine, 2011; 1: 146-152. (In Russian)

30. Yaremchuk M.M., Dyka M.V., Sanagursky D.I. The activity of prooxidant-antioxidant system in loach embryos under the action of microwave radiation. Ukrainian Biochemical Journal, 2014; 86(5): 142-150. (In Ukrainian)
https://doi.org/10.15407/ubj86.05.142

31. Yaremchuk M.M., Mandzynets S.M., Dyka M.V. et al. Microwave radiation influence on the change of Na+, K+-ATPase activity of loach embryos. Biophysical Bulletin, 2013; 30(2): 95-101. (In Ukrainian)


Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Studia biologica