OBTAINING HUMAN HAIR KERATIN-BASED FILMS AND THEIR CHARACTERISTICS
DOI: http://dx.doi.org/10.30970/sbi.1501.643
Abstract
Background. Keratins are natural biopolymers with a wide range of applications in the field of biotechnology.
Materials and Methods. Extraction of keratins was performed by a modified Nakamura method using 250 mM DTT. The protein concentration in the supernatant was determined by Bradford method. The protein composition was studied by their electrophoretic separation in a polyacrylamide gel in the presence of sodium dodecyl sulfate. The films were made by casting. The surface characteristics of the films were determined using a scanning electron microscope REMMA-102. The elemental composition of the films was determined using an X-ray microanalyzer.
Results. The protein concentration in the supernatant was 3.75 mg/mL. After using dithiothreitol in the extraction mixture, we obtained proteins of intermediate filaments with a molecular weight of 40–60 kDa and a low Sulfur content. In the low molecular weight region, we obtained keratin-associated proteins with a molecular weight of 10–30 kDa and a high content of Sulfur. These proteins belong to fibrillar proteins, which can be used as a matrix for the creation of new keratin-containing biocomposites with a wide range of applications in reparative medicine and tissue engineering.
Based on the obtained keratin extract, polymer films with and without the addition of glycerol were made. Scanning electron microscopy revealed that glycerol provided the film structure with homogeneity and plasticity due to the accumulation of moisture after the fixation by water vapor.
The X-ray microanalysis of films revealed such elements as Sodium, Silicon, Sulfur, Potassium. Among the detected elements, Sulfur has the largest share that is due to the large number of disulfide bonds in the keratin molecule.
Conclusions. The polymer keratin films with the addition of glycerol demonstrated better mechanical properties and can be used in biomedicine.
Keywords
Full Text:
PDFReferences
| 1. Aigner T.B., DeSimone E., Scheibel T. Biomedical applications of recombinant silk-based materials. Advanced Materials, 2018; 30(19): 1704636. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 2. Alashwal B.Y., Saad Bala M., Gupta A., Sharma S., Mishra P. Improved properties of keratin-based bioplastic film blended with microcrystalline cellulose: A comparative analysis. Journal of King Saud University - Science, 2020; 32(1): 853-857. Crossref ● Google Scholar | ||||
| ||||
| 3. Bhardwaj N., Sow W.T., Devi D., Ng K.W., Mandal B.B., Cho N.J. Silk fibroin-keratin based 3D scaffolds as a dermal substitute for skin tissue engineering. Integrative Biology, 2015; 7(1): 53-63. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 4. Cochis A., Ferraris S., Sorrentino R., Azzimonti B., Novara C., Geobaldo F., Rimondini L. Silver-doped keratin nanofibers preserve a titanium surface from biofilm contamination and favor soft-tissue healing. Journal of Materials Chemistry B, 2017; 5(42): 8366-8377. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 5. Costa F., Silva R., Boccaccini A.R. Fibrous protein-based biomaterials (silk, keratin, elastin, and resilin proteins) for tissue regeneration and repair. Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair, 2018; 175-204. Crossref ● Google Scholar | ||||
| ||||
| 6. Feng Y., Borrelli M., Meyer-Ter-Vehn T., Reichl S., Schrader S., Geerling G. Epithelial wound healing on keratin film, amniotic membrane and polystyrene in vitro. Current Eye Research, 2014; 39(6): 561-570. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 7. Feroz S., Muhammad N., Ranayake J., Dias G. Keratin - Based materials for biomedical applications. Bioactive Materials, 2020; 5(3): 496-509. Crossref ● PubMed ● PMC ● Google Scholar | ||||
| ||||
| 8. Gupta P., Nayak K.K. Characteristics of protein-based biopolymer and its application. Polymer Engineering & Science, 2015; 55(3): 485-498. Crossref ● Google Scholar | ||||
| ||||
| 9. Kavitha A., Boopalan K., Radhakrishnan G., Sankaran S., Das B.N., Sastry T.P. Preparation of feather keratin hydrolyzate-gelatin composites and their graft copolymers. Journal of Macromolecular Science, Part A, 2005; 42(12): 1703-1713. Crossref ● Google Scholar | ||||
| ||||
| 10. Martelli S.M., Moore G., Paes S.S., Gandolfo C., Laurindo J.B. Influence of plasticizers on the water sorption isotherms and water vapor permeability of chicken feather keratin films. LWT - Food Science and Technology, 2006; 39(3): 292-301. Crossref ● Google Scholar | ||||
| ||||
| 11. McLellan J., Thornhill S.G., Shelton S., Kumar M. Keratin-Based Biofilms, Hydrogels, and Biofibers. In: Sharma S., Kumar A. (eds) Keratin as a Protein Biopolymer. Springer Series on Polymer and Composite Materials. Springer, Cham., 2019; 187-200. Crossref ● Google Scholar | ||||
| ||||
| 12. Moore G.R.P., Martelli S.M., Gandolfo C., Sobral P.J.A., Laurindo J.B. Influence of the glycerol concentration on some physical properties of feather keratin films. Food Hydrocolloids, 2006; 20(7): 975-982. Crossref ● Google Scholar | ||||
| ||||
| 13. Nakamura A., Arimoto M., Takeuchi K., Fujii T. A rapid extraction procedure of human hair proteins and identification of phosphorylated species. Biological and Pharmaceutical Bulletin, 2002; 25(5): 569-572. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 14. Posati T., Giuri D., Nocchetti M., Sagnella A., Gariboldi M., Ferroni C., Aluigi A. Keratin-hydrotalcites hybrid films for drug delivery applications. European Polymer Journal, 2018; 105: 177-185. Crossref ● Google Scholar | ||||
| ||||
| 15. Reichl S. Films based on human hair keratin as substrates for cell culture and tissue engineering. Biomaterials, 2009; 30(36): 6854-6866. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 16. Shavandi A., Silva T.H., Bekhit A.A., Bekhit A.E.-D.A. Keratin: dissolution, extraction and biomedical application. Biomaterials Science, 2017; 5(9): 1699-1735. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 17. Tanabe T., Okitsu N., Tachibana A., Yamauchi K. Preparation and characterization of keratin-chitosan composite film. Biomaterials, 2002; 23(3): 817-825. Crossref ● Google Scholar | ||||
| ||||
| 18. Thonpho A., Srihanam P. Preparation and сharacterization of keratin blended films using biopolymers for drug controlled release application. Oriental Journal of Chemistry, 2016; 32(4): 1739-1748. Crossref ● Google Scholar | ||||
| ||||
| 19. Tonin C., Aluigi A., Vineis C., Varesano A., Montarsolo A., Ferrero F. Thermal and structural characterization of poly(ethylene-oxide)/keratin blend films. Journal of Thermal Analysis and Calorimetry, 2007; 89: 601-608. Crossref ● Google Scholar | ||||
| ||||
| 20. Vaz J.M., Pezzoli D., Chevallier P., Campelo C.S., Candiani G., Mantovani D. Antibacterial coatings based on chitosan for pharmaceutical and biomedical applications. Current pharmaceutical design, 2018; 24(8): 866-885. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 21. Yamauchi K., Yamauchi A., Kusunoki T., Kohda A., Konishi Y. Preparation of stable aqueous solution of keratins, and physiochemical and biodegradational properties of films. Journal of Biomedical Materials Research, 1996; 31: 439-444. Crossref ● Google Scholar | ||||
| ||||
| 22. Yin X.-C., Li F.-Y., He Y.-F., Wang Y., Wang R.-M. Study on effective extraction of chicken feather keratins and their films for controlling drug release. Biomaterials Science, 2013; 1(5): 528. Crossref ● PubMed ● Google Scholar | ||||
| ||||
| 23. Zhuang Y., Wu X., Cao Z., Zhao X., Zhou M., Gao P. Preparation and characterization of sponge film made from feathers. Materials Science and Engineering: C, 2013; 33(8): 4732-4738. Crossref ● PubMed ● Google Scholar | ||||
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 V. V. Mykhaliuk, V. V. Havryliak
This work is licensed under a Creative Commons Attribution 4.0 International License.