SPECTRA DETALIZATION OF BLOOD PLASMA FREE OLIGOSACCHARIDES OF HEALTHY VOLUNTEERS

Abstract

Free oligosaccharides of blood plasma of healthy volunteers were separated into fractions of uncharged (neutral) and charged (acidic) glycans with ion-exchange chromatography on QAE-Sephadex. Glycans of the fractions were analyzed by normal-phase high performance liquid chromatography (HPLC). It has been shown that most of the free oligosaccharides are uncharged glycans concentrated in three main peaks of the HPLC-profiles and consisted of 5–7 monosaccharide residues. The spectrum of charged oligosaccharides has been characterized by one main peak of glycans with 8–9 monosaccharide residues and by the structures included from 5 to 12 monosaccharide residues, more diverse in composition, but with a low concentration.