CONTENT OF PRIMARY AND SECONDARY PRODUCTS OF LIPID PEROXIDATION IN LOACH EMBRYOS UNDER THE EFFECT OF FLURENIZYD
DOI: http://dx.doi.org/10.30970/sbi.1001.444
Abstract
The effect of flurenizide (antibiotic of antimicrobial, antituberculousis, antichlamydia, immunomodulator, antioxidant, hepatoprotective, antiinflammatory, antiviral action) on the intensity of lipid peroxidation processes of embryos Misgurnus fossilis L. at the stage of 2, 16, 64, 256 and 1024 blastomeres was investigated. Flurenizide in all studied concentrations (0.01; 0.05; 0.15; 1; 5; 15 mM) caused increase in content of lipid peroxidation primary products (hydroperoxides) during development of embryos but decrease on stage 256 blastomeres. The content of secondary products of lipid peroxidation (TBA-positive products) increased during the development of embryos under the effect of antibiotic in concentration of 0.01÷1 mM, and decreased at the concentration 15 mM. It was found that loach embryos were the most sensitive to flurenizide at the stage of 16 blastomeres when the content of hydroperoxides is the maximum. The less sensitive stage of development of germ cells to the exogenous factors was 256 blastomeres.
Keywords
Full Text:
PDF (Українська)References
1. Dubinina E.E. Role of reactive oxygen species as signaling molecules in the metabolism tissues in a state of oxidative stress. Problems of Medical Chemistry, 2001; 76(6): 136-141. (In Russian) | |
| |
2. Lowry O. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 1951; 193(1): 404-415. (In Russian) | |
| |
3. Menshchikova E. Oxidative stress. Prooxidants and antioxidants. Moscow: Word, 2006. 556 p. (In Russian) | |
| |
4. Mihalik О. Modern drugs for chemotherapy of viral infections: a guide. Lviv, 2013. 180 p. (In Ukrainian) | |
| |
5. Neyfah A., Timofeeva M. Problems in molecular biology regulation development. Moscow: Nauka, 1978. 336 p. (In Russian) | |
| |
6. Oleksjuk N., Janovych V. Activity of pro- and antioxidant systems in the liver of freshwater fishes in different seasons. The Ukrainian Biochemical Journal, 2010; 82(3): 41-48. (In Ukrainian) | |
| |
7. Petrukh L. Fluorene as tuberkulostatik. Flurenizyd: microbiological pharmacological and clinical aspects. Lviv, 2008; 464-469. (In Ukrainian) | |
| |
8. Petrukh L. Flurenizyd veterinary practice: Coll. materials of international scientific-practical conference "Modern problems of veterinary medicine, zooengineering animal products and technologies". Lviv, 1997. P. 216-217. (In Ukrainian) | |
| |
9. Petrukh L. Рharmaceutical education and language. The achievements of pharmaceutical research. Lviv, 2011. 152 p. (In Ukrainian) | |
| |
10. Petrukh L. The urgency of the creation and implementation of industrial production of new medicines. Collection descriptions of inventions. Lviv, 2003. 198 p. (In Ukrainian) | |
| |
11. Petrukh L. Urgency creation and implementation in the industrial production of new drugs. Collection of descriptions of inventions. Lviv, 2003; 196 p. (In Ukrainian) | |
| |
12. Sanagurskiy D.I. Biophysics objects. Lviv: Publishing House of Ivan Franko LNU, 2008. 522 p. (In Ukrainian) | |
| |
13. Timirbulatov R., Seleznev Е. Method of increase intensity of free-radical oxidization of lipid components of blood and his diagnostic value. Laboratory Business, 1981; 4: 209-211. (In Russian) | |
| |
14. Zyn A., Golovchak N., Tarnovska A., Galan M., Sanagurski D. Effect of sodium hypochlorite on prooxidant and antioxidant homeostasis of loach embryos during early embryogenesis. Studia Biologica, 2012; 6(1): 67-76. (In Ukrainian) | |
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Studia biologica
This work is licensed under a Creative Commons Attribution 4.0 International License.