NANOSIZED TITANIUM DIOXIDE MATERIAL. MODULATION OF SPONTANEOUS MOTILITY AND GABA-DEPENDENT REGULATION OF FUNCTIONS OF STOMACH SMOOTH MUSCLES  IN VIVO

A. M. Naumenko; O. V. Tsymbalуuk; M. A. Skoryk; I. S. Voiteshenko; V. A. Skryshevsky; T. L. Davydovska

doi:10.30970/sbi.1101.520

NANOSIZED TITANIUM DIOXIDE MATERIAL. MODULATION OF SPONTANEOUS MOTILITY AND GABA-DEPENDENT REGULATION OF FUNCTIONS OF STOMACH SMOOTH MUSCLES IN VIVO

A. M. Naumenko, O. V. Tsymbalуuk, M. A. Skoryk, I. S. Voiteshenko, V. A. Skryshevsky, T. L. Davydovska

DOI: http://dx.doi.org/10.30970/sbi.1101.520

Abstract

Electron scanning microscopy was used to obtain the image and identify the size of TiO₂ nanoparticles. Using the tenzometric method in the isometric mode, it was established that chronic effect of TiO₂ on stomach smooth muscles led to redistribution of the amplitudes of spontaneous contractions in terms of their frequencies. An increase in frequency of their contractions, decrease in the duration of contraction–relaxation cycle, the disturbance of the asymmetry of the duration of contraction–relaxation development,
a reduction in Montevideo index of contractions and Alexandria index of contractions were demonstrated. It was also shown the existance of the divergence in numerical values of frequency-amplitude complexes, TiO₂-modified spontaneous contractive activity of smooth muscles of stomach and caecum. In the conditions of long-term chronic influence (100 days), TiO₂ removes the regulatory mechanisms of depressing a release of inhibition neuromediators from neurons of the intramural nervous interlacement, mediated by GABA_A- and GABA_C-receptors, in smooth muscles of stomach and caecum.

Keywords

stomach smooth muscle, titanium dioxide nanoparticles, mechanokinetics, GABA-dependent regulation, spontaneous and induced contractions

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References

1. Bayer S., Jellali A., Crenner F. et al. Functional evidence for a role of GABA receptors in modulating nerve activities of circular smooth muscle from rat colon in vitro. Life Sciences, 2003; 72(13): 1481-1493. https://doi.org/10.1016/S0024-3205(02)02413-X

2. Boeckxstaens G.E., Pelckmans P.A., Rampart M. et al. GABAA receptor-mediated stimulation of non-adrenergic non-cholinergic neurons in the dog ileocolonic junction. Br. J. Pharmacol, 1990; 101: 460-464. https://doi.org/10.1111/j.1476-5381.1990.tb12730.x PMid:2175237 PMCid:PMC1917714

3. Bolton T.V., Zholos A.V. Activation of M2 muscarinic receptors in guinea - pig ileum opens cationic chnnels modulated by M3 muscarinic receptors. Life Sciences, 1997; 60: 1121-1128. https://doi.org/10.1016/S0024-3205(97)00056-8

4. Burdyga Th.V., Kosterin S.A. Kinetic analysis of smooth muscle relaxation. Gen Physiol Biophys, 1991; 10(6): 589-598.

5. Eglen R.M. Muscarinic receptors and gastrointestinal tract smooth muscle function. Life Sciences, 2001; 68: 2573-2578. https://doi.org/10.1016/S0024-3205(01)01054-2

6. Furness J.B. The enteric nervous system and neurogastroenterology. Nat. Rev. Gastroenterol. Hepatol, 2012; 9(5): 286-294. https://doi.org/10.1038/nrgastro.2012.32 PMid:22392290

7. Khalili Fard J., Jafari S., Eghbal M.A. A review of molecular mechanisms involved in toxicity of nanoparticles. Advanced Pharmaceutical Bulletin, 2015; 5(4), 447-454. https://doi.org/10.15171/apb.2015.061 PMid:26819915 PMCid:PMC4729339

8. Kurjak M., Fichna J., Harbarth J. et al. Effect of GABA-ergic mechanisms on synaptosomal NO synthesis and the nitrergic component of NANC relaxation in rat ileum. Neurogastroenterol Motil, 2011; 23(5): e181-e190. https://doi.org/10.1111/j.1365-2982.2011.01688.x PMid:21414101

9. Lomax A.E., Furness J.B. Neurochemical classification of enteric neurons in the guinea-pig distal colon. Cell Tissue Res, 2000; 302(1): 59-72. https://doi.org/10.1007/s004410000260 PMid:11079716

10. Myronyuk I.F., Chelyadyn V.L. Methods of titanium dioxide obtaining. Physics and Chemistry of Solid State, 2010; 11(4): 815-831. (In Ukrainian)

11. Nasіbyan L.S., Fіlіppov I.B. Modulation of rat myometrium contractions via Staphylococcus aureus cell wall peptidoglycan. Physiol. Journal, 2014; 60: 62-72. https://doi.org/10.15407/fz60.05.062 PMid:25566672

12. Naumenko A.M., Nyporko A.Yu., Tsymbalyuk O.V. et al. Molecular docking of nanosized titanium dioxide material to the extracellular part of GABAB-receptor. Studia Biologica, 2016; 10(3-4): 5-16. https://doi.org/10.30970/sbi.1003.506

13. Nyporko A.Yu., Naumenko A.M., Tsymbalyuk O.V. et al. Three-dimensional reconstruction of a full-size GABAB receptor. Neurophysiology, 2015; 5: 44-53. https://doi.org/10.1007/s11062-016-9544-3

14. Powell J.J., Faria N., Thomas-McKay E., Pele L.C. Origin and fate of dietary nanoparticles and microparticles in the gastrointestinal tract. Journal of Autoimmunity, 2010; 34(3): J226-J233. https://doi.org/10.1016/j.jaut.2009.11.006 PMid:20096538

15. Sayre R.M., Dowdy J.C. Titanium dioxide and zinc oxide induce photooxidation of unsaturated lipids. Cosmetics and Toiletries, 2000; 115(10): 75-82.

16. Shi H., Magaye R., Castranova V., Zhao J. Titanium dioxide nanoparticles: a review of current toxicological data. Particle and Fibre Toxicology, 2013; 10: 15. https://doi.org/10.1186/1743-8977-10-15 PMid:23587290 PMCid:PMC3637140

17. Song B., Liu J., Feng X. et al. A review on potential neurotoxicity of titanium dioxide nanoparticles. Nanoscale Research Letters, 2015; 10(1): 342. https://doi.org/10.1186/s11671-015-1042-9 PMid:26306536 PMCid:PMC4549355

18. Tada-Oikawa S., Ichihara G., Fukatsu H. et al. Titanium Dioxide Particle Type and Concentration Influence the Inflammatory Response in Caco-2 Cells. International Journal of Molecular Sciences, 2016; 17(4): 576. https://doi.org/10.3390/ijms17040576 PMid:27092499 PMCid:PMC4849032

19. Tsymbalyuk O.V., Naumenko A.M., Nyporko O.Yu. et al. The excitation-inhibition of smooth muscles of stomach at the interaction with nanosized material of titanium dioxide. Reports of the National Academy of Sciences of Ukraine, 2015; 10: 85-92. (In Ukrainian) https://doi.org/10.15407/dopovidi2015.10.085

20. Tsymbalyuk O.V., Naumenko A.M., Rohovtsov O.S. et al. Titanium dioxide modulation of the contractibility of visceral smooth muscles in vivo. Nanoscale Research Letters, 2017; 12: 129. https://doi.org/10.1186/s11671-017-1865-7 PMid:28235365 PMCid:PMC5318306

21. Tsymbalyuk O.V., Naumenko A.M., Skoryk M.A. et al. Histamine- and nicotine-stimulated modulations of mechanic activity of smooth muscles in gastrointestinal tract at the impact of nanosized TiO2 material. Biopolymers & Cell, 2016; 32(2): 140-149. https://doi.org/10.7124/bc.000917

22. Wang Y., Chen Z., Ba T. et al. Susceptibility of young and adult rats to the oral toxicity of titanium dioxide nanoparticles. Small, 2013; 9(9-10): 1742-1752. https://doi.org/10.1002/smll.201201185 PMid:22945798

23. Ward S.M., McLaren G.J., Sanders K.M. Interstitial cells of Cajal in the deep muscular plexus mediate enteric motor neurotransmission in the mouse small intestine. J. Physiol, 2006; 573(Pt 1): 147-159. https://doi.org/10.1113/jphysiol.2006.105189 PMid:16513671 PMCid:PMC1779710

24. Weir A., Westerhoff P., Fabricius L. et al. Titanium dioxide nanoparticles in food and personal care products. Environmental Science Technology, 2012; 46(4): 2242-2250. https://doi.org/10.1021/es204168d PMid:22260395 PMCid:PMC3288463

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