THE ANTIOXIDANT EFFECT OF NATURAL POLYPHENOLIC COMPLEXES OF GRAPE WINE IN THE RAT RETINA UNDER STREPTOZOTOCIN-INDUCED DIABETES MELLITUS

A. R. Gnatush, V. R. Drel, A. Ya. Yalaneckyy, V. I. Mizin, V. A. Zagoruyko, V. G. Gerzhykova, N. O. Sybirna


DOI: http://dx.doi.org/10.30970/sbi.0502.156

Abstract


The protective effects of natural polyphenolic complexes of the grape wine on antioxidant system in retinae of rats under streptozotocin-induced diabetes mellitus are established. It was shown that proposed level of polyphenol speciment of the grape wine that was consumed did not change blood glucose concentrations in control and diabetic groups of animals. During the experiment the body weight of control group and diabetic groups of rats with consumption of studied speciment was significantly increased. The polyphenols completely or partially normalized the work of enzymatic antioxidant system (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase). It was shown that level of products of lipid peroxidation in the groups of rats with and without consumption of polyphenol speciment differed significantly. The presented results allow to suggest an important role of speciment of grape wine in preventing and treatment of diabetes retinopathy.


Keywords


diabetes mellitus, polyphenolic complexes, diabetes retinopathy, oxidative stress, antioxidant protection

References


1. Crawford T.N., Alfaro D.V. 3rd, Kerrison J.B., Jablon E.P. Diabetic retinopathy and angiogenesis. Curr. Diabetes Rev, 2009; 5(1): 8-13.
https://doi.org/10.2174/157339909787314149

2. Daglia M., Papetti A., Grisoli P. et al. Antibacterial activity of red and white wine against oral streptococcin. J. Agric. Food Chem, 2007; 55 (13): 5038-5042.
https://doi.org/10.1021/jf070352q
PMid:17547418

3. Frank R.N. Diabetic retinopathy. N. Engl. J. Med, 2004; 350: 48-58.
https://doi.org/10.1056/NEJMra021678
PMid:14702427

4. Goldberg D.M., Spooner R.J., Bergmeyer H.U. Methods of Enzymatic Analysis, 3rd еd. Weinheim: Verlag Chemie. 1983; 5(3): 258-265.

5. Gouni-Berthold I., Krone W. Diabetic ketoacidosis and hyperosmolar hyperglycemic State. Med. Klin. (Munich), 2006; 101 (Suppl 1): 100-105.
https://doi.org/10.1016/B978-1-4160-0273-4.50014-X

6. Kato N., Yashima S., Suzuki T. et al. Long-term treatment with fidarestat suppresses the development of diabetic retinopathy in STZ-induced diabetic rats. Diabetes Complications, 2003; 17: 374-379.
https://doi.org/10.1016/S1056-8727(02)00193-9

7. Kitabchi A.E., Umpierrez G.E., Fisher J.N. et al. Thirty years of personal experience in hyperglycemic crises: diabetic ketoacidosis and hyperglycemic hyperosmolar state. Journ. of Clinical Endocrinology and Metabolism, 2008; 93(5): 1541-1552.
https://doi.org/10.1210/jc.2007-2577
PMid:18270259 PMCid:PMC2386681

8. Lowri O.H., Rosenbraugh M.J., Pori A.L Protein measurement with the Folin phenol reagent. Biol. Chem, 1951; 193(1): 265-275.

9. Marfella R., Cacciapuoti F., Siniscalchi M. et al. Effect of moderate red wine intake on cardiac prognosis after recent acute myocardial infarction of subjects with Type 2 diabetes mellitus. Diabet Med, 2006; 23(9): 974-981.
https://doi.org/10.1111/j.1464-5491.2006.01886.x
PMid:16922703

10. Montilla P., Barcos M., Munoz M.C. et al. Red wine prevents brain oxidative stress and nephropathy in streptozotocin-induced diabetic rats. J. Biochem. Mol. Biol, 2005; 38(5): 539-544.
https://doi.org/10.5483/BMBRep.2005.38.5.539

11. Napoli C., Balestrieri M.L., Sica V. et al. Beneficial effects of low doses of red wine consumption on perturbed shear stress-induced atherogenesis. Heart Vessels, 2008; 23(2): 124-133.
https://doi.org/10.1007/s00380-007-1015-8
PMid:18389338

12. Tapp R.J., Shaw J.E., Harper C.A. et al. AusDiab Study Group. The prevalence of and factors associated with diabetic retinopathy in the Australian population. Diabetes Care, 2003; 26(6): 1731-1737.
https://doi.org/10.2337/diacare.26.6.1731
PMid:12766102

13. Viktor R. Drel, Nataliya Sybirna. Protective effects of polyphenolics in red wine on diabetes associated oxidative/nitrative stress in streptozotocindiabetic rats. Cell Biol. Int, 2010; 34: 1147-1153.
https://doi.org/10.1042/CBI20100201
PMid:20662768

14. Бездетко А. В., Горбачева К. С. Эпидемиология и частота сахарного диабета и диабетической ретинопатии. Междунар. эндокрин. журн, 2006; 4(6): 76-80.

15. Дрель В.Р., Гнатуш А.Р., Яланецький А.Я. та ін. Поліфеноли виноградних вин запобігають накопиченню нітротирозину та активації parp-1 у сітківці ока щурів із стрептозотоцин-індукованим цукровим діабетом. Мед. хімія, 2010; 1(42): 2-33.

16. Іванушко Я.Г., Гриневич Ю.П., Липська А.І. Вплив рентгенівського випромінення на ферменти антиокиснювального захисту печінки щурів. Ядерна фізика та енергетика, 2009; 10(1): 76-79.

17. Королюк М.А., Иванова И.Г., Майорова И.Г. Метод определения активности каталазы. Лаб. дело, 1988; 1: 16-18.

18. Меньшикова Е. Б., Ланкин В. З., Зенков Н. К. и др. Окислительный стресс. Прооксиданты и антиоксиданты. Москва, 2006: 411-413.

19. Моин В. М. Простой и специфический метод определения активности глутатионпероксидазы в эритроцитах. Лаб. дело, 1986; 12: 124-126.

20. Сибірна Н.О., Маєвська О.М., Барська М.Л. Дослідження окремих біохімічних показників за умов оксидативного стресу: Навч.-метод. посібник. Львів: Видавн. центр ЛНУ ім. І. Франка, 2006. 60 с.

21. Тимирбулатов Р.А., Селезнев Е.И. Метод повышения интенсивности свободнорадикального окисления липидсодержащих компонентов крови и его диагностическое значение. Лаб. дело, 1981; 4: 209-211.

22. Чевари С., Андял Т.Д., Штиренгер Д. Определение антиоксидантных параметров крови и их диагностическое значение в преклонном возрасте. Лаб. дело, 1991; 10: 9-13.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2011 Studia biologica

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.