APOPTOTIC MODIFICATION OF GLYCOSPHINGOLIPIDS OF HUMAN GRANULOCYTES
DOI: http://dx.doi.org/10.30970/sbi.0602.220
Abstract
The phenomenon of programmed apoptotic desialylation of cell glycans was described previously. However, the possibility of this process application to the sialylated glycolipids of membranes stayes unknown. Granulocytes of human peripherical blood were utilized as a model for studying glycosphingolipid spectra changes. Ganglioside fractions were extracted from the granulocytes and purified by two-stage column chromatography – DEAE-Fractogel and Sephadex LH-20 sorbents, with subsequent analysis by thin-layer and high-performance liquid chromatography. An increased level of GM2, GM1 ganglioside and Gb3 globoside in apoptotic cells, as well as a decrease of GM3, GT3 and Gb4 amount were detected. These results draw to a conclusion that gangliosides, as well as N-glycans, might be a target of apoptotic desialylation. According to available recent data, changes in gangliosides can serve as important pathogenesis factors in the autoimmune disorders.
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