IMMOBILIZATION OF OXIDOREDUCTASES ON GOLD AND SILVER NANOPARTICLES
DOI: http://dx.doi.org/10.30970/sbi.0803.365
Abstract
Nanoparticles of silver and gold were synthesized by chemical methods. Nanoparticles of silver were obtained by reducing of silvernitrate with glucose and nanoparticles of gold – by reducing of tetrachloroauric acid trihydrate with sodium citrate or borohydride. New methods of functionalization of nanoparticles surface were proposed. To activate nanoparticles, two approaches were applied: nanoparticles of silver and gold synthesized by citrate method were treated with 16-mercaptohexadecanoic acid, and nanoparticles of gold obtained with the help of sodium borohydride method, were functionalized with cysteamine. Bionanomaterials were obtained by immobilizing of horse radish peroxidase and glucose oxidase of Penicillium adametzii on the activated nanoparticles. Scanning electron microscopy and spectrophotometric methods proved nanosizes of resulting nanoparticles conjugated with enzymes. The study of catalytic properties of enzymes immobilized on the nanoparticles showed that nanoparticles of gold are the most effective for peroxidase, whereas the nanoparticles of silver are optimal carriers for conjugation with glucose oxidase.
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1. Alaa A.A. Aljabali, David J. Evans. Polyelectrolyte-Modified Cowpea Mosaic Virus for the Synthesis of Gold Nanoparticles. Virus Hybrids as Nanomaterials, 2014; 1108: 97-103. | |
| |
2. Ansar S.M., Ameer F.S., Hu W. et al. Removal of molecular adsorbates on gold nanoparticles using sodium borohydride in water. Nano Lett, 2013; 13(3) 1226-1229. | |
| |
3. Arya S.K., Saha S., Ramirez-Vick J.E. et al. Recent advances in ZnO nanostructures and thin films for biosensors applications. Review. Anal. Chim. Acta, 2012; 737: 1-21. | |
| |
4. Berezin I.V., Antonov V.K., Martinek K. Immobilized enzymes. Moscow: MSU, 1976: 1-2. (In Russian) | |
| |
5. Cade N.I., Ritman-Meer T., Kwakwa K.A. et al. The plasmonic engineering of metal nanoparticles for enhanced fluorescence and Raman scattering. Nanotechnol, 2009; 20: 285201-285206. | |
| |
6. Chen Y., Aveyard J., Wilson R. Gold and silver nanoparticles functionalized with known numbers of oligonucleotides per particle for DNA detection. Chem. Commun, 2004; 24: 2804-2805. | |
| |
7. Dhillon G.S., Brar S.K., Kaur S., Verma M. Green approach for nanoparticle biosynthesis by fungi: current trends and applications. Crit. Rev. Biotechnol, 2012; 32(1): 49-73. | |
| |
8. Dongxiang L., Qiang H., Yue C. Immobilization of glucose oxidase onto gold nanoparticles with enhanced thermostability. BBRC, 2007; 355: 488-493. | |
| |
9. Doria G., Conde J., Veigas B. et al. Noble metal nanoparticles for biosensing applications. Sensors, 2012; 12(2): 1657-1687. | |
| |
10. Iosin M., Baldeck P., Astilean S. Study of tryptophan assisted synthesis of gold nanoparticles by combining UV-Vis, fluorescence, and SERS spectroscopy. Journal of Nanoparticle Research, 2010; 12(8): 2843-2849. | |
| |
11. Kamiar A., Ghotalou R., Vali Z.H. Preparation, physicochemical characterization and performance evaluation of gold nanoparticles in radiotherapy. Adv. Pharm. Bull, 2013;3(2): 425-428. | |
| |
12. Kharissova O.V., Dias H.V., Kharisov B.I. et al. The greener synthesis of nanoparticles. Trends Biotechnol, 2013; 31(4): 240-248. | |
| |
13. Kumar S., Gandhi K.S., Kumar R. Modeling of formation of gold nanoparticles by Citrate Method. Ind. Eng. Chem. Res, 2007; 46: 3136-3138. | |
| |
14. Maribel G., Guzmon M.G., Dille J. et al. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. World Acad. Sci. Eng. Tech, 2008; 43: 357-364. | |
| |
15. Narasimha G., Alzohairy M., Khadri H., Mallikarjuna K. Extracellular synthesis, characterization and antibacterial activity of Silver nanoparticles by Actinomycetes isolative. Int. J. Nano Dimens, 2013; 4(1): 77-83. | |
| |
16. Narayanan K.B., Sakthivel N. Biological synthesis of metal nanoparticles by microbes. Advances in Colloid and Interface Science, 2010; 156(1-2): 1-13. | |
| |
17. Park H.H., Zhang X., Choi Y.J. et al. Synthesis of Ag nanostructures by Photochemical reduction using citrate-capped Pt seeds. J. Nanomaterials, 2011; 1: 1-7. | |
| |
18. Patra H.K., Banerjee S., Chaudhuri U. et al. Cell selective response to gold nanoparticles. NanoMed, 2007; 3(2): 111-119. | |
| |
19. Park Y., Hong Y.N., Weyers A. et al. Polysaccharides and phytochemicals: a natural reservoir for the green synthesis of gold and silver nanoparticles. IET Nanobiotechnol, 2011; 5(3): 69-78. | |
| |
20. Salvadori M.R., Ando R.A., Oller do Nascimento C.A, Correa B. Intracellular Biosynthesis and Removal of Copper Nanoparticles by Dead Biomass of Yeast Isolated from the Wastewater of a Mine in the Brazilian Amazonia. BioMedLib, 2014; 9(1): 1-9. | |
| |
21. Shang Y., Min C., Hu J. et al. Synthesis of gold nanoparticles by reduction of HAuCl4 under UV irradiation. Solid State Sciences, 2013, 15: 17-23. | |
| |
22. Stasyuk N., Serkiz R., Mudry S. et al. Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties. Nanotech. Develop, 2011; 1(3): 11-15. | |
| |
23. Stasyuk N., Smutok O., Gayda G. et al. A new bi-enzyme potentiometric sensor for arginine analysis based on recombinant human arginase I and commercial urease. J. Mater. Sci. Eng. A, 2011; 1: 819-827. | |
| |
24. Xu J., Han X., Liu H. et al. Synthesis of monodisperse gold nanoparticles stabilized by gemini surfactant in reverse micelles. J. Disper. Sci. Technol, 2005; 26: 473-476. | |
| |
25. Yeh I.C., Creran B., Rotello V.M. Gold nanoparticles: preparation, properties, and applications in bionanotechnology. Nanoscale, 2012; 4: 1871-1880. | |
| |
26. Zhang X., Xing J.Z., Chen J. et al. Enhanced radiation sensitivity in prostate cancer by gold-nanoparticles. Clin. Invest. Med, 2008; 31(3): 160-167. |
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